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Protein

L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase

Gene

mshC

Organism
Corynebacterium glutamicum (strain R)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent condensation of GlcN-Ins and L-cysteine to form L-Cys-GlcN-Ins.UniRule annotation

Catalytic activityi

1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP = 1-O-(2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + AMP + diphosphate.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi48ZincUniRule annotation1
Binding sitei63Cysteinyl adenylateUniRule annotation1
Binding sitei232Cysteinyl adenylateUniRule annotation1
Metal bindingi236ZincUniRule annotation1
Metal bindingi261ZincUniRule annotation1
Binding sitei288Cysteinyl adenylate; via amide nitrogen and carbonyl oxygenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCGLU340322:G1G2L-1674-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligaseUniRule annotation (EC:6.3.1.13UniRule annotation)
Short name:
L-Cys:GlcN-Ins ligaseUniRule annotation
Alternative name(s):
Mycothiol ligaseUniRule annotation
Short name:
MSH ligaseUniRule annotation
Gene namesi
Name:mshCUniRule annotation
Ordered Locus Names:cgR_1574
OrganismiCorynebacterium glutamicum (strain R)
Taxonomic identifieri340322 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
Proteomesi
  • UP000006698 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004004381 – 420L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligaseAdd BLAST420

Proteomic databases

PRIDEiA4QEA0

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA4QEA0
SMRiA4QEA0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni48 – 51Cysteinyl adenylate bindingUniRule annotation4
Regioni86 – 88Cysteinyl adenylate bindingUniRule annotation3
Regioni254 – 256Cysteinyl adenylate bindingUniRule annotation3

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi50 – 60"HIGH" regionUniRule annotationAdd BLAST11
Motifi192 – 197"ERGGDP" regionUniRule annotation6
Motifi294 – 298"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. MshC subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000245251
KOiK15526
OMAiIFPHHEM
OrthoDBiPOG091H004K

Family and domain databases

Gene3Di3.40.50.620, 1 hit
HAMAPiMF_01697 MshC, 1 hit
InterProiView protein in InterPro
IPR024909 Cys-tRNA/MSH_ligase
IPR017812 Mycothiol_ligase_MshC
IPR014729 Rossmann-like_a/b/a_fold
IPR032678 tRNA-synt_1_cat_dom
PANTHERiPTHR10890 PTHR10890, 1 hit
PTHR10890:SF13 PTHR10890:SF13, 1 hit
PfamiView protein in Pfam
PF01406 tRNA-synt_1e, 1 hit
PRINTSiPR00983 TRNASYNTHCYS
TIGRFAMsiTIGR03447 mycothiol_MshC, 1 hit

Sequencei

Sequence statusi: Complete.

A4QEA0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQSWPTPEVP ALAGTPVPLE LFDTADQEVR LVETPPAGSD TPVGMYVCGI
60 70 80 90 100
TPYDSTHLGH AATYLAFDLI YRILLDNDHD VHYVQNITDV DDPLFERAAR
110 120 130 140 150
DGVDWRDLGT SQINLFRSDM EALSIIPPKD YIGAIESIDE VIEMVKTLLD
160 170 180 190 200
EGAAYIVEDA EYPDVYASIN ATDKFGYESN YDAATMAEFF AERGGDPDRP
210 220 230 240 250
GKKNPMDALL WRAAREGEPS WESPFGAGRP GWHIECSAIA TNRLGHSFDI
260 270 280 290 300
QGGGSDLIFP HHEFSAAHAE AAHGVERMAK HYVHAGMISQ DGVKMSKSLG
310 320 330 340 350
NLEFVSRLTA AGHEPGAIRL GVFANHYRGN RDWNAESLAT AEQRLATWRE
360 370 380 390 400
AARAATNRED AIAVVEQLRA HLSADLDTPG ALAAVDNWAA GIDTTAGSKE
410 420
FTEVGNIVVA AIDALLGVQL
Length:420
Mass (Da):45,645
Last modified:May 15, 2007 - v1
Checksum:i3118E5AC1FD03934
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009044 Genomic DNA Translation: BAF54566.1
RefSeqiWP_011897265.1, NC_009342.1

Genome annotation databases

EnsemblBacteriaiBAF54566; BAF54566; cgR_1574
KEGGicgt:cgR_1574

Entry informationi

Entry nameiMSHC_CORGB
AccessioniPrimary (citable) accession number: A4QEA0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: May 15, 2007
Last modified: May 23, 2018
This is version 66 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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