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Protein

L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase

Gene

mshC

Organism
Corynebacterium glutamicum (strain R)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent condensation of GlcN-Ins and L-cysteine to form L-Cys-GlcN-Ins.UniRule annotation

Catalytic activityi

1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP = 1-O-(2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + AMP + diphosphate.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi48 – 481ZincUniRule annotation
Binding sitei63 – 631Cysteinyl adenylateUniRule annotation
Binding sitei232 – 2321Cysteinyl adenylateUniRule annotation
Metal bindingi236 – 2361ZincUniRule annotation
Metal bindingi261 – 2611ZincUniRule annotation
Binding sitei288 – 2881Cysteinyl adenylate; via amide nitrogen and carbonyl oxygenUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCGLU340322:GJBE-1646-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligaseUniRule annotation (EC:6.3.1.13UniRule annotation)
Short name:
L-Cys:GlcN-Ins ligaseUniRule annotation
Alternative name(s):
Mycothiol ligaseUniRule annotation
Short name:
MSH ligaseUniRule annotation
Gene namesi
Name:mshCUniRule annotation
Ordered Locus Names:cgR_1574
OrganismiCorynebacterium glutamicum (strain R)
Taxonomic identifieri340322 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
Proteomesi
  • UP000006698 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 420420L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligasePRO_0000400438Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA4QEA0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni48 – 514Cysteinyl adenylate bindingUniRule annotation
Regioni86 – 883Cysteinyl adenylate bindingUniRule annotation
Regioni254 – 2563Cysteinyl adenylate bindingUniRule annotation

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi50 – 6011"HIGH" regionUniRule annotationAdd
BLAST
Motifi192 – 1976"ERGGDP" regionUniRule annotation
Motifi294 – 2985"KMSKS" regionUniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. MshC subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000245251.
KOiK15526.
OMAiILAHHYR.
OrthoDBiPOG091H004K.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_01697. MshC. 1 hit.
InterProiIPR024909. Cys-tRNA/MSH_ligase.
IPR017812. Mycothiol_ligase_MshC.
IPR014729. Rossmann-like_a/b/a_fold.
IPR032678. tRNA-synt_1_cat_dom.
[Graphical view]
PANTHERiPTHR10890. PTHR10890. 1 hit.
PfamiPF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSiPR00983. TRNASYNTHCYS.
TIGRFAMsiTIGR03447. mycothiol_MshC. 1 hit.

Sequencei

Sequence statusi: Complete.

A4QEA0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQSWPTPEVP ALAGTPVPLE LFDTADQEVR LVETPPAGSD TPVGMYVCGI
60 70 80 90 100
TPYDSTHLGH AATYLAFDLI YRILLDNDHD VHYVQNITDV DDPLFERAAR
110 120 130 140 150
DGVDWRDLGT SQINLFRSDM EALSIIPPKD YIGAIESIDE VIEMVKTLLD
160 170 180 190 200
EGAAYIVEDA EYPDVYASIN ATDKFGYESN YDAATMAEFF AERGGDPDRP
210 220 230 240 250
GKKNPMDALL WRAAREGEPS WESPFGAGRP GWHIECSAIA TNRLGHSFDI
260 270 280 290 300
QGGGSDLIFP HHEFSAAHAE AAHGVERMAK HYVHAGMISQ DGVKMSKSLG
310 320 330 340 350
NLEFVSRLTA AGHEPGAIRL GVFANHYRGN RDWNAESLAT AEQRLATWRE
360 370 380 390 400
AARAATNRED AIAVVEQLRA HLSADLDTPG ALAAVDNWAA GIDTTAGSKE
410 420
FTEVGNIVVA AIDALLGVQL
Length:420
Mass (Da):45,645
Last modified:May 15, 2007 - v1
Checksum:i3118E5AC1FD03934
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009044 Genomic DNA. Translation: BAF54566.1.
RefSeqiWP_011897265.1. NC_009342.1.

Genome annotation databases

EnsemblBacteriaiBAF54566; BAF54566; cgR_1574.
KEGGicgt:cgR_1574.
PATRICi21507740. VBICorGlu58097_1623.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009044 Genomic DNA. Translation: BAF54566.1.
RefSeqiWP_011897265.1. NC_009342.1.

3D structure databases

ProteinModelPortaliA4QEA0.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAF54566; BAF54566; cgR_1574.
KEGGicgt:cgR_1574.
PATRICi21507740. VBICorGlu58097_1623.

Phylogenomic databases

HOGENOMiHOG000245251.
KOiK15526.
OMAiILAHHYR.
OrthoDBiPOG091H004K.

Enzyme and pathway databases

BioCyciCGLU340322:GJBE-1646-MONOMER.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_01697. MshC. 1 hit.
InterProiIPR024909. Cys-tRNA/MSH_ligase.
IPR017812. Mycothiol_ligase_MshC.
IPR014729. Rossmann-like_a/b/a_fold.
IPR032678. tRNA-synt_1_cat_dom.
[Graphical view]
PANTHERiPTHR10890. PTHR10890. 1 hit.
PfamiPF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSiPR00983. TRNASYNTHCYS.
TIGRFAMsiTIGR03447. mycothiol_MshC. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMSHC_CORGB
AccessioniPrimary (citable) accession number: A4QEA0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: May 15, 2007
Last modified: September 7, 2016
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.