A4QDM9 (ILVD_CORGB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase
Short name=DAD
EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	cgR_1344

Organism

Corynebacterium glutamicum (strain R) [Complete proteome] [HAMAP]

Taxonomic identifier

340322 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	613 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 613	613	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_1000000978
Sites
	Metal binding	122	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	197	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	A4QDM9 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: B42F6E43D1ADB250	FASTA	613	64,673
	10 20 30 40 50 60 MIPLRSKVTT VGRNAAGARA LWRATGTKEN EFGKPIVAIV NSYTQFVPGH VHLKNVGDIV 70 80 90 100 110 120 ADAVRKAGGV PKEFNTIAVD DGIAMGHGGM LYSLPSREII ADSVEYMVNA HTADAMVCIS 130 140 150 160 170 180 NCDKITPGML NAAMRLNIPV VFVSGGPMEA GKAVVVDGVA HAPTDLITAI SASASDAVDD 190 200 210 220 230 240 AGLAAVEASA CPTCGSCSGM FTANSMNCLT EALGLSLPGN GSTLATHAAR RALFEKAGET 250 260 270 280 290 300 VVELCRRYYG EEDESVLPRG IATKKAFENA MALDMAMGGS TNTILHILAA AQEGEVDFDL 310 320 330 340 350 360 ADIDELSKNV PCLSKVAPNS DYHMEDVHRA GGIPALLGEL NRGGLLNKDV HSVHSNDLEG 370 380 390 400 410 420 WLDDWDIRSG KTTEVATELF HAAPGGIRTT EAFSTENRWD ELDTDAAKGC IRDVEHAYTA 430 440 450 460 470 480 DGGLVVLRGN ISPDGAVIKS AGIEEELWNF TGPARVVESQ EEAVSVILTK TIQAGEVLVV 490 500 510 520 530 540 RYEGPSGGPG MQEMLHPTAF LKGSGLGKKC ALITDGRFSG GSSGLSIGHV SPEAAHGGVI 550 560 570 580 590 600 GLIENGDIVS IDVHNRKLEV QVSNEELQRR RDAMNASEKP WQPVNRNRVV TKALRAYAKM 610 ATSADKGAVR QVD

References

[1]

"Comparative analysis of the Corynebacterium glutamicum group and complete genome sequence of strain R." Yukawa H., Omumasaba C.A., Nonaka H., Kos P., Okai N., Suzuki N., Suda M., Tsuge Y., Watanabe J., Ikeda Y., Vertes A.A., Inui M. Microbiology 153:1042-1058(2007) [PubMed: 17379713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: R.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP009044 Genomic DNA. Translation: BAF54326.1.
RefSeq	YP_001138228.1. NC_009342.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A4QDM9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4991726.
GenomeReviews	Gene locus cgR_1344 in contig AP009044_GR.
KEGG	cgt:cgR_1344.
PATRIC	21507240. VBICorGlu58097_1383.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0129.
HOGENOM	HBG671001.
OMA	NSHNEII.
ProtClustDB	PRK12448.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_CORGB

Accession

Primary (citable) accession number: A4QDM9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 15, 2007
Last modified:	December 14, 2011
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program