A4QDI6 (A4QDI6_CORGB) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
1,4-alpha-glucan branching enzyme GlgB HAMAP MF_00685
EC=2.4.1.18 HAMAP MF_00685

Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase HAMAP MF_00685
Alpha-(1->4)-glucan branching enzyme HAMAP MF_00685
Glycogen branching enzyme HAMAP MF_00685

Gene names

Name:	glgB HAMAP MF_00685
Ordered Locus Names:	cgR_1302

Organism

Corynebacterium glutamicum (strain R) [Complete proteome] [HAMAP]

Taxonomic identifier

340322 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	731 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP MF_00685
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685 SAAS SAAS006407
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685 SAAS SAAS006407
Subunit structure	Monomer By similarity. HAMAP MF_00685
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. GlgB subfamily. HAMAP MF_00685

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen biosynthesis HAMAP MF_00685 SAAS SAAS006407 Glycogen metabolism HAMAP MF_00685
Molecular function	Glycosyltransferase HAMAP MF_00685 SAAS SAAS006407 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: HAMAP cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	408	1	Nucleophile By similarity HAMAP MF_00685
	Active site	461	1	Proton donor By similarity HAMAP MF_00685

Sequences

	Sequence		Length	Mass (Da)
	A4QDI6 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: B25F5EEF1D32B1DE	FASTA	731	82,690
	10 20 30 40 50 60 MTVDPASHIT IPEADLARLR HCNHHDPHGF YGWHETEAGS VIRTRQIGAT QVNLLIDDTS 70 80 90 100 110 120 HVMTPIGDDI FAIDLRHRER ADYRLEVTWP DQEPQVKADP YYFLPTVGEM DIYLFSEGRH 130 140 150 160 170 180 ERLWEVLGAN IKTYQTALGT VRGTAFTVWA PNAIGCAVVG GFNGWNASQH PMRSMGGSGL 190 200 210 220 230 240 WELFIPGIEE GEVYKFALQT KEGHRRDKAD PMARRAELAP ATGSIVASSE YQWQDSEWLR 250 260 270 280 290 300 ERSQTDLPSK PMSVYEVHLG SWRWGKNYED LATELVDYVA DLGYTHVEFL PVAEHPFGGS 310 320 330 340 350 360 WGYQVTGYYA PTSRWGTPDQ FRALVDAFHA RGIGVIMDWV PAHFPKDDWA LARFDGEALY 370 380 390 400 410 420 EHPDWRRGEQ KDWGTLVFDF GRNEVRNFLV ANALYWIEEF HIDGLRVDAV ASMLYLDYSR 430 440 450 460 470 480 EHGEWEPNIY GGRENLEAVQ FLQEMNATVL RLHPGALTIA EESTSWPGVT APTWDGGLGF 490 500 510 520 530 540 SLKWNMGWMN DTLEYFSKNP VHRAFHHSEL TFSLVYAFSE RFVLPISHDE VVHGKGSLWD 550 560 570 580 590 600 RMPGDTWNKA AGLRTFLAYM WSHPGKKLLF MGQEFGQREE WAEGQGLPWD IVDGWQGEYH 610 620 630 640 650 660 EAIRTLTRSL NGVYSDSPAL HTQDFTGEGF TWNKGDDATN NILAFTRFGS DGSQMLCVFN 670 680 690 700 710 720 LSGTSQPEYQ LGVAAGGEWK LVLNTDDAEF LGAENDIATS VQAAATPRDN FAYSLSLHVP 730 AMSAQFYSLQ K

References

[1]

"Comparative analysis of the Corynebacterium glutamicum group and complete genome sequence of strain R." Yukawa H., Omumasaba C.A., Nonaka H., Kos P., Okai N., Suzuki N., Suda M., Tsuge Y., Watanabe J., Ikeda Y., Vertes A.A., Inui M. Microbiology 153:1042-1058(2007) [PubMed: 17379713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: R.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP009044 Genomic DNA. Translation: BAF54283.1.
RefSeq	YP_001138185.1. NC_009342.1.
3D structure databases
ProteinModelPortal	A4QDI6.
ModBase	Search...
Protein-protein interaction databases
STRING	A4QDI6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4992176.
GenomeReviews	Gene locus cgR_1302 in contig AP009044_GR.
KEGG	cgt:cgR_1302.
PATRIC	21507154. VBICorGlu58097_1340.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0296.
HOGENOM	HBG287139.
OMA	DGTCLYE.
ProtClustDB	PRK05402.
Family and domain databases
HAMAP	MF_00685. GlgB. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz. IPR006048. A-amylase_b_C. IPR015902. Alpha_amylase. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 2 hits.
KO	K00700.
PANTHER	PTHR10357. Alpha_amylase. 1 hit. PTHR10357:SF13. PTHR10357:SF13. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
PIRSF	PIRSF000463. GlgB. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit. SSF81296. Ig_E-set. 2 hits.
TIGRFAMs	TIGR01515. Branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A4QDI6_CORGB

Accession

Primary (citable) accession number: A4QDI6

Entry history

Integrated into UniProtKB/TrEMBL:	May 15, 2007
Last sequence update:	May 15, 2007
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)