Reviewed, UniProtKB/Swiss-Prot A4QDA4 (METE_CORGB)

Names and origin

Protein names

Recommended name:
    5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
    EC=2.1.1.14
Alternative name(s):
    Methionine synthase, vitamin-B12 independent isozyme
    Cobalamin-independent methionine synthase

Gene names

Name:	metE
Ordered Locus Names:	cgR_1223

Organism

Corynebacterium glutamicum (strain R) [Complete proteome] [HAMAP]

Taxonomic identifier

340322 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	745 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity.
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 745	745	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_1000017239
Sites
	Metal binding	628	1	Zinc By similarity
	Metal binding	630	1	Zinc By similarity
	Metal binding	713	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4QDA4-1 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: 6FC0C8FE9D380713	FASTA	745	81,254
	10 20 30 40 50 60 MTSNFSSTVA GLPRIGAKRE LKFALEGYWN GSIEGRELAQ TARQLVNTAS DSLSGLDSVP 70 80 90 100 110 120 FAGRSYYDAM LDTAAILGVL PERFDDIADH ENDGLPLWID RYFGAARGTE TLPAQAMTKW 130 140 150 160 170 180 FDTNYHYLVP ELSADTRFVL DASALIEDLR CQQVRGVNAR PVLVGPLTFL SLARTTDGSN 190 200 210 220 230 240 PLDHLPALFE VYERLIKSFD TEWVQIDEPA LVTDVAPEVL EQVRAGYTTL AKRGGVFVNT 250 260 270 280 290 300 YFGSGDQALN TLAGIGLGAI GVDLVTHGVT ELAAWKGEEL LVAGIVDGRN IWRTDLCAAL 310 320 330 340 350 360 ASLKRLAARG PIAVSTSCSL LHVPYTLEAE NIEPEVRDWL AFGSEKITEV KLLADALAGN 370 380 390 400 410 420 IDAAAFDAAS AAIASRRTSP RTAPITQELP GRSRGSFNTR VTLQEKSLEL PALPTTTIGS 430 440 450 460 470 480 FPQTPSIRSA RARLRKESIT LEQYEEAMRE EIDLVIAKQE ELGLDVLVHG EPERNDMVQY 490 500 510 520 530 540 FSELLDGFLS TANGWVQSYG SRCVRPPVLF GNVSRPAPMT VKWFQYAQSL TQKHVKGMLT 550 560 570 580 590 600 GPVTILAWSF VRDDQPLATT ADQVALALRD EINDLIEAGA KIIQVDEPAI RELLPLRDVD 610 620 630 640 650 660 KPAYLQWSVD SFRLATAGAP DDVQIHTHMC YSEFNEVISS VIALDADVTT IEAARSDMQV 670 680 690 700 710 720 LAALKSSGFE LGVGPGVWDI HSPRVPSAQE VDGLLEAALQ SVDPRQLWVN PDCGLKTRGW 730 740 PEVEASLKVL VESAKQAREK IGATI

References

[1]

"Comparative analysis of the Corynebacterium glutamicum group and complete genome sequence of strain R." Yukawa H., Omumasaba C.A., Nonaka H., Kos P., Okai N., Suzuki N., Suda M., Tsuge Y., Watanabe J., Ikeda Y., Vertes A.A., Inui M. Microbiology 153:1042-1058(2007) [PubMed: 17379713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP009044 Genomic DNA. Translation: BAF54201.1.
RefSeq	YP_001138103.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A4QDA4.
Genome annotation databases
GeneID	4993780.
GenomeReviews	Gene locus cgR_1223 in contig AP009044_GR.
KEGG	cgt:cgR_1223.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	CSLLHTP.
Family and domain databases
HAMAP	MF_00172. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR002629. Methionine_synth. IPR006276. MeTrfase_B12_ind. [Graphical view]
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
ProDom	PD004692. Methionine_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01371. met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_CORGB

Accession

Primary (citable) accession number: A4QDA4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 15, 2007
Last modified:	November 3, 2009
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)