A4QB65 (AROE_CORGB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Quinate/shikimate dehydrogenase
EC=1.1.1.-
EC=1.1.1.24

Alternative name(s):
NAD(+)-dependent quinate dehydrogenase
Short name=QDH

Gene names

Name:	aroE
Ordered Locus Names:	cgR_0495

Organism

Corynebacterium glutamicum (strain R) [Complete proteome] [HAMAP]

Taxonomic identifier

340322 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	283 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NAD+-dependent oxidation of both quinate and shikimate to 3-dehydroquinate and 3-dehydroshikimate, respectively. Seems to play a key role in the quinate degradation pathway By similarity. HAMAP MF_00222
Catalytic activity	Quinate + NAD+ = 3-dehydroquinate + NADH. HAMAP MF_00222 Shikimate + NAD+ = 3-dehydroshikimate + NADH. HAMAP MF_00222
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7. HAMAP MF_00222
Subunit structure	Homodimer By similarity. HAMAP MF_00222
Sequence similarities	Belongs to the shikimate dehydrogenase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	nucleotide binding Inferred from electronic annotation. Source: InterPro quinate 3-dehydrogenase (NAD+) activity Inferred from electronic annotation. Source: EC shikimate 3-dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 283	283	Quinate/shikimate dehydrogenase HAMAP MF_00222		PRO_0000351451
Regions
	Nucleotide binding	134 – 138	5	NAD By similarity
	Nucleotide binding	251 – 255	5	NAD By similarity
Sites
	Active site	73	1	Proton acceptor Potential
	Binding site	110	1	Substrate By similarity
	Binding site	158	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4QB65 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: 82490DA71877A4A2	FASTA	283	29,710
	10 20 30 40 50 60 MNDSILLGLI GQGLDLSRTP AMHEAEGLAQ GRATVYRRID TLGSRASGQD LKTLLDAALY 70 80 90 100 110 120 LGFNGLNITH PYKQAVLPLL DEVSEQATQL GAVNTVVIDA NGHTTGHNTD VSGFGRGMEE 130 140 150 160 170 180 GLPNAKLDSV VQVGAGGVGN AVAYALVTHG VQKLQVADLD TSRAQALADV INNAVGREAV 190 200 210 220 230 240 VGVDARGIED VIAAADGVVN ATPMGMPAHP GTAFDVSCLT KDHWVGDVVY MPIETELLKA 250 260 270 280 ARALGCETLD GTRMAIHQAV DAFRLFTGLE PDVSRMRETF LSL

References

[1]

"Comparative analysis of the Corynebacterium glutamicum group and complete genome sequence of strain R." Yukawa H., Omumasaba C.A., Nonaka H., Kos P., Okai N., Suzuki N., Suda M., Tsuge Y., Watanabe J., Ikeda Y., Vertes A.A., Inui M. Microbiology 153:1042-1058(2007) [PubMed: 17379713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: R.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP009044 Genomic DNA. Translation: BAF53462.1.
RefSeq	YP_001137364.1. NC_009342.1.
3D structure databases
ProteinModelPortal	A4QB65.
SMR	A4QB65. Positions 3-283.
ModBase	Search...
Protein-protein interaction databases
STRING	A4QB65.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4994338.
GenomeReviews	Gene locus cgR_0495 in contig AP009044_GR.
KEGG	cgt:cgR_0495.
PATRIC	21505477. VBICorGlu58097_0518.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0169.
HOGENOM	HBG553408.
OMA	HEHEGDA.
ProtClustDB	PRK14027.
Family and domain databases
HAMAP	MF_00222. Shikimate_DH_AroE. Divergent sequence. [Tree]
InterPro	IPR016040. NAD(P)-bd_dom. IPR013708. Shikimate_DH-bd_N. IPR022893. Shikimate_quinate_DH. IPR006151. Shikm_DH/Glu-tRNA_Rdtase. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00014.
Pfam	PF01488. Shikimate_DH. 1 hit. PF08501. Shikimate_dh_N. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROE_CORGB

Accession

Primary (citable) accession number: A4QB65

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 14, 2008
Last sequence update:	May 15, 2007
Last modified:	December 14, 2011
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program