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Protein

D-inositol 3-phosphate glycosyltransferase

Gene

mshA

Organism
Corynebacterium glutamicum (strain R)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a N-acetyl-glucosamine moiety to 1D-myo-inositol 3-phosphate to produce 1D-myo-inositol 2-acetamido-2-deoxy-glucopyranoside 3-phosphate in the mycothiol biosynthesis pathway.UniRule annotation

Catalytic activityi

UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate = UDP + 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei9 – 911D-inositol 3-phosphateUniRule annotation
Binding sitei23 – 231UDP-GlcNAc; via amide nitrogenUniRule annotation
Binding sitei78 – 7811D-inositol 3-phosphateUniRule annotation
Binding sitei110 – 11011D-inositol 3-phosphateUniRule annotation
Binding sitei134 – 13411D-inositol 3-phosphateUniRule annotation
Binding sitei154 – 15411D-inositol 3-phosphateUniRule annotation
Binding sitei231 – 2311UDP-GlcNAcUniRule annotation
Binding sitei236 – 2361UDP-GlcNAcUniRule annotation
Binding sitei294 – 2941UDP-GlcNAc; via amide nitrogen and carbonyl oxygenUniRule annotation
Metal bindingi303 – 3031Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi304 – 3041Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi306 – 3061Magnesium; via carbonyl oxygenUniRule annotation
Binding sitei316 – 3161UDP-GlcNAcUniRule annotation
Binding sitei324 – 3241UDP-GlcNAcUniRule annotation
Metal bindingi330 – 3301MagnesiumUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciCGLU340322:GJBE-493-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
D-inositol 3-phosphate glycosyltransferase (EC:2.4.1.250UniRule annotation)
Alternative name(s):
N-acetylglucosamine-inositol-phosphate N-acetylglucosaminyltransferaseUniRule annotation
Short name:
GlcNAc-Ins-P N-acetylglucosaminyltransferaseUniRule annotation
Gene namesi
Name:mshAUniRule annotation
Ordered Locus Names:cgR_0473
OrganismiCorynebacterium glutamicum (strain R)
Taxonomic identifieri340322 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
Proteomesi
  • UP000006698 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 418418D-inositol 3-phosphate glycosyltransferasePRO_0000400118Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA4QB40.
SMRiA4QB40. Positions 1-409.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni15 – 162UDP-GlcNAc bindingUniRule annotation
Regioni20 – 2561D-inositol 3-phosphate bindingUniRule annotation

Sequence similaritiesi

Belongs to the glycosyltransferase group 1 family. MshA subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000077288.
KOiK15521.
OMAiHTMAKVK.
OrthoDBiEOG647TVR.

Family and domain databases

HAMAPiMF_01695. MshA.
InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
IPR017814. Mycothiol_biosynthesis_MshA.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03449. mycothiol_MshA. 1 hit.

Sequencei

Sequence statusi: Complete.

A4QB40-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVAMISMHT SPLQQPGTGD SGGMNVYILS TATELAKQGI EVDIYTRATR
60 70 80 90 100
PSQGEIVRVA ENLRVINIAA GPYEGLSKEE LPTQLAAFTG GMLSFTRREK
110 120 130 140 150
VTYDLIHSHY WLSGQVGWLL RDLWRIPLIH TAHTLAAVKN SYRDDSDTPE
160 170 180 190 200
SEARRICEQQ LVDNADVLAV NTQEEMQDLM HHYDADPDRI SVVSPGADVE
210 220 230 240 250
LYSPGNDRAT ERSRRELGIP LHTKVVAFVG RLQPFKGPQV LIKAVAALFD
260 270 280 290 300
RDPDRNLRVI ICGGPSGPNA TPDTYRHMAE ELGVEKRIRF LDPRPPSELV
310 320 330 340 350
AVYRAADIVA VPSFNESFGL VAMEAQASGT PVIAARVGGL PIAVAEGETG
360 370 380 390 400
LLVDGHSPHA WADALATLLD DDETRIRMGE DAVEHARTFS WAATAAQLSS
410
LYNDAIANEN VDGETHHG
Length:418
Mass (Da):45,669
Last modified:May 15, 2007 - v1
Checksum:i3865B32C25DB6C07
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009044 Genomic DNA. Translation: BAF53437.1.
RefSeqiWP_011013628.1. NC_009342.1.

Genome annotation databases

EnsemblBacteriaiBAF53437; BAF53437; cgR_0473.
KEGGicgt:cgR_0473.
PATRICi21505433. VBICorGlu58097_0496.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009044 Genomic DNA. Translation: BAF53437.1.
RefSeqiWP_011013628.1. NC_009342.1.

3D structure databases

ProteinModelPortaliA4QB40.
SMRiA4QB40. Positions 1-409.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAF53437; BAF53437; cgR_0473.
KEGGicgt:cgR_0473.
PATRICi21505433. VBICorGlu58097_0496.

Phylogenomic databases

HOGENOMiHOG000077288.
KOiK15521.
OMAiHTMAKVK.
OrthoDBiEOG647TVR.

Enzyme and pathway databases

BioCyciCGLU340322:GJBE-493-MONOMER.

Family and domain databases

HAMAPiMF_01695. MshA.
InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
IPR017814. Mycothiol_biosynthesis_MshA.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03449. mycothiol_MshA. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Comparative analysis of the Corynebacterium glutamicum group and complete genome sequence of strain R."
    Yukawa H., Omumasaba C.A., Nonaka H., Kos P., Okai N., Suzuki N., Suda M., Tsuge Y., Watanabe J., Ikeda Y., Vertes A.A., Inui M.
    Microbiology 153:1042-1058(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: R.

Entry informationi

Entry nameiMSHA_CORGB
AccessioniPrimary (citable) accession number: A4QB40
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: May 15, 2007
Last modified: May 11, 2016
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.