A4QAW2 (A4QAW2_CORGB) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA topoisomerase RuleBase RU000518
EC=5.99.1.2 RuleBase RU000518

Gene names

Ordered Locus Names:

cgR_0395

Organism

Corynebacterium glutamicum (strain R) [Complete proteome] [HAMAP]

Taxonomic identifier

340322 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	1004 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. RuleBase RU000518 SAAS SAAS005733
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family. RuleBase RU004206 Contains 1 Toprim domain. SAAS SAAS023406

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS005733 DNA-binding SAAS SAAS005733 RuleBase RU000518 Nucleotide-binding
Molecular function	Isomerase Topoisomerase RuleBase RU000518 SAAS SAAS005733
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	A4QAW2 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: EAA156D30A7A5F11	FASTA	1,004	110,251
	10 20 30 40 50 60 MADTAGTTGT AGSKKKYLVI VESATKAKKI QPYLGNDYIV EASVGHIRDL PRGAADIPAK 70 80 90 100 110 120 YKKEPWARLG VDTDRGFAPL YVVSPDKKKK VADLKAKLKL VDELLLATDP DREGEAIAWH 130 140 150 160 170 180 LLEVLKPTVP VRRMVFNEIT KPAILAAAEN TRELDENLVD AQETRRILDR LYGYEVSPVL 190 200 210 220 230 240 WKKVMPRLSA GRVQSVATRV IVERERERMA FVSADYWDLS AEFNAGESGK ADSDNPSSFT 250 260 270 280 290 300 ARLSTIDGNR VAQGRDFNDR GELTSEAVVV DKQRAEALAE ALEGQEMAVV GVEEKPYTRR 310 320 330 340 350 360 PYAPFMTSTL QQESGRKLHY TSERTMRIAQ RLYENGHITY MRTDSTSLSE QGMKAARDQA 370 380 390 400 410 420 LELYGAEYVS PSPRTYDRKV KNSQEAHEAI RPAGETFATP GQLHGQLDAE EFKLYELIWQ 430 440 450 460 470 480 RTVASQMADA KGTSMKVTIG GTAKTGEKTE FNATGRTLTF PGFLRAYVET TRTADGRDVA 490 500 510 520 530 540 DNAEKRLPLL SEGDLLKVLG IEADGHSTNP PARYTEASLV KKMEDLGIGR PSTYASIIKT 550 560 570 580 590 600 IQDRGYVYSR GNALVPSWVA FAVVGLLEAN FTSLVDYDFT SSMEDELDNI AAGREGRTEW 610 620 630 640 650 660 LNGFYFGDAE ADQSMAESVA RQGGLKALVD ANLEHIDARS VNSLKLFDDA EGRAVNVRVG 670 680 690 700 710 720 RYGPYIERIV GTTAEGEPEF QRANLPEETT PDELTLEVAE KLFATPQGGR ELGINPANGR 730 740 750 760 770 780 MVVAKEGRFG PYVIEQVTDS ERAGAEAQAE EVVAAERKAE DEQRAADGMR PKNWETKTAA 790 800 810 820 830 840 NQKEKRINQL VEENLKPATA SLFSGMEPAT VTLEEALKLL SLPREVGVDP SDNEVITAQN 850 860 870 880 890 900 GRYGPYLKKG SDSRSLNSEE QIFTVTLDEA RRIYAEPKRR GRAAAQPPLK QLGDNDVSGK 910 920 930 940 950 960 PMTVKDGRFG PYVTDGTTNA SLRKGDVPES LTDARANELL SERRAKEAAD GGAPAKKTST 970 980 990 1000 KKTAAKKTTA KKTTAKKTTA KKTVRKAPPK TTKNVVKAGA RKKS

References

[1]

"Comparative analysis of the Corynebacterium glutamicum group and complete genome sequence of strain R." Yukawa H., Omumasaba C.A., Nonaka H., Kos P., Okai N., Suzuki N., Suda M., Tsuge Y., Watanabe J., Ikeda Y., Vertes A.A., Inui M. Microbiology 153:1042-1058(2007) [PubMed: 17379713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: R.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP009044 Genomic DNA. Translation: BAF53359.1.
RefSeq	YP_001137261.1. NC_009342.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A4QAW2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4993913.
GenomeReviews	Gene locus cgR_0395 in contig AP009044_GR.
KEGG	cgt:cgR_0395.
PATRIC	21505255. VBICorGlu58097_0408.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0550.
HOGENOM	HBG512935.
OMA	KKVMPRL.
ProtClustDB	PRK07561.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

A4QAW2_CORGB

Accession

Primary (citable) accession number: A4QAW2

Entry history

Integrated into UniProtKB/TrEMBL:	May 15, 2007
Last sequence update:	May 15, 2007
Last modified:	January 25, 2012
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)