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Protein

Protein polyglycylase TTLL10

Gene

Ttll10

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Polyglycylase which modifies both tubulin and non-tubulin proteins, generating side chains of glycine on the gamma-carboxyl groups of specific glutamate residues of target proteins. Polyglycylates alpha-tubulin and beta-tubulin, but is not able to initiate glycylation and only has activity toward monoglycylated tubulin. Has the ability to polyglycylate non-tubulin proteins such as NAP1; in this case it can initiate glycylation and does not require preliminary monoglycylation by another glycylase.3 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei363 – 3631ATPBy similarity
Binding sitei365 – 3651ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi350 – 3534ATP bindingBy similarity

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein-glycine ligase activity Source: UniProtKB
  • protein-glycine ligase activity, elongating Source: UniProtKB

GO - Biological processi

  • protein polyglycylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein polyglycylase TTLL10 (EC:6.3.2.-)
Alternative name(s):
Tubulin--tyrosine ligase-like protein 10
Gene namesi
Name:Ttll10
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1921855. Ttll10.

Subcellular locationi

GO - Cellular componenti

  • axoneme Source: UniProtKB
  • cilium Source: UniProtKB
  • cytoplasm Source: UniProtKB-KW
  • microtubule Source: UniProtKB-KW
  • microtubule cytoskeleton Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cilium, Cytoplasm, Cytoskeleton, Microtubule

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi499 – 4991E → V: Abolishes polyglycylase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 704704Protein polyglycylase TTLL10PRO_0000324522Add
BLAST

Proteomic databases

PaxDbiA4Q9F3.
PRIDEiA4Q9F3.

PTM databases

iPTMnetiA4Q9F3.
PhosphoSiteiA4Q9F3.

Expressioni

Gene expression databases

CleanExiMM_TTLL10.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000055671.

Structurei

3D structure databases

ProteinModelPortaliA4Q9F3.
SMRiA4Q9F3. Positions 272-505.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini169 – 540372TTLPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi142 – 1454Poly-Glu
Compositional biasi570 – 61243Pro-richAdd
BLAST

Sequence similaritiesi

Contains 1 TTL domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2157. Eukaryota.
ENOG410XQDM. LUCA.
HOGENOMiHOG000049156.
HOVERGENiHBG108614.
InParanoidiA4Q9F3.
PhylomeDBiA4Q9F3.

Family and domain databases

InterProiIPR004344. TTL/TTLL_fam.
IPR027752. TTLL10.
[Graphical view]
PANTHERiPTHR12241:SF110. PTHR12241:SF110. 1 hit.
PfamiPF03133. TTL. 1 hit.
[Graphical view]
PROSITEiPS51221. TTL. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: A4Q9F3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALHPQAGRP HRDGSEAQAE AAAQDLGRLP SPSKVGAAVC RIQGLGHRAA
60 70 80 90 100
RRPRRGIGTT SASRVPRPGA LMPATRNRPR FIHCRGQPPR TRVSSKRSKR
110 120 130 140 150
SRIHPCHTEV PGWTHEKQMG SSVKERLRPE LSQLDQDADD LEEEEAARLP
160 170 180 190 200
VTSPDGLLME GDKQPSPGQG PFFYIGGTNG ASIISNYCES KGWQRTQDSH
210 220 230 240 250
CEDYKLKWCE IKCRDNYCSF REGQQLLFQL PNNKLLTTKI GLLSALREHA
260 270 280 290 300
RTLSKARMLP STQTKVLKME EFFPETYRLD IRDERQAFFA LFDETQMWIC
310 320 330 340 350
KPTASNQGKG IFLIRSQEEA AALQAKTQSI EDDPIYRKMP FRAPQARVVQ
360 370 380 390 400
RYVQNPLLLD GKKFDVRSYM LIACAMPYMV FFGHGYARLT LSLYNPHSSD
410 420 430 440 450
LSGHLTNQFM QKKSPLYTLL KESTVWTMEH LNRYINDKFR KTKGLPRDWV
460 470 480 490 500
FTTFTKRMQQ IMSHCFLAVK SKLECKLGYF DLIGCDFLID ENFKVWLLEM
510 520 530 540 550
NANPALHTNC EVLKAVIPGV VIETLDLALE TCQKSLHSQK MLPLLSQRRF
560 570 580 590 600
VLLYNGETTD LWPRLASSRP LNRLPNPNPN PNPNANPHPH PHPNPHPHPN
610 620 630 640 650
PHPNANPHPP RPTCEAASSA LSSARAAISE RPGARKSMPS RGAPVCTPRK
660 670 680 690 700
SRLSDSSGSS IAESEPSLCS GSLEGSRDTA REPSLGPPEE EREEEQRSTS

HRGS
Length:704
Mass (Da):79,250
Last modified:May 15, 2007 - v1
Checksum:i3225E8C26C760B1F
GO
Isoform 2 (identifier: A4Q9F3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     15-44: SEAQAEAAAQDLGRLPSPSKVGAAVCRIQG → R

Note: No experimental confirmation available.
Show »
Length:675
Mass (Da):76,443
Checksum:iC1B59F56574FA70A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti578 – 5781N → H in BAE21500 (PubMed:16141072).Curated
Sequence conflicti585 – 5862AN → PH in BAE21500 (PubMed:16141072).Curated
Sequence conflicti596 – 5961H → N in BAE21500 (PubMed:16141072).Curated
Sequence conflicti600 – 6001N → H in BAE21500 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei15 – 4430SEAQA…CRIQG → R in isoform 2. 1 PublicationVSP_032265Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM690754 mRNA. Translation: CAM84331.1.
AK133079 mRNA. Translation: BAE21500.1.
CCDSiCCDS19058.1. [A4Q9F3-2]
RefSeqiNP_083540.1. NM_029264.2.
XP_006539036.2. XM_006538973.2.
UniGeneiMm.440232.

Genome annotation databases

GeneIDi330010.
KEGGimmu:330010.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM690754 mRNA. Translation: CAM84331.1.
AK133079 mRNA. Translation: BAE21500.1.
CCDSiCCDS19058.1. [A4Q9F3-2]
RefSeqiNP_083540.1. NM_029264.2.
XP_006539036.2. XM_006538973.2.
UniGeneiMm.440232.

3D structure databases

ProteinModelPortaliA4Q9F3.
SMRiA4Q9F3. Positions 272-505.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000055671.

PTM databases

iPTMnetiA4Q9F3.
PhosphoSiteiA4Q9F3.

Proteomic databases

PaxDbiA4Q9F3.
PRIDEiA4Q9F3.

Protocols and materials databases

DNASUi330010.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi330010.
KEGGimmu:330010.

Organism-specific databases

CTDi254173.
MGIiMGI:1921855. Ttll10.

Phylogenomic databases

eggNOGiKOG2157. Eukaryota.
ENOG410XQDM. LUCA.
HOGENOMiHOG000049156.
HOVERGENiHBG108614.
InParanoidiA4Q9F3.
PhylomeDBiA4Q9F3.

Miscellaneous databases

PROiA4Q9F3.
SOURCEiSearch...

Gene expression databases

CleanExiMM_TTLL10.

Family and domain databases

InterProiIPR004344. TTL/TTLL_fam.
IPR027752. TTLL10.
[Graphical view]
PANTHERiPTHR12241:SF110. PTHR12241:SF110. 1 hit.
PfamiPF03133. TTL. 1 hit.
[Graphical view]
PROSITEiPS51221. TTL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A targeted multienzyme mechanism for selective microtubule polyglutamylation."
    van Dijk J., Rogowski K., Miro J., Lacroix B., Edde B., Janke C.
    Mol. Cell 26:437-448(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Testis.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Testis.
  3. "Evolutionary divergence of enzymatic mechanisms for posttranslational polyglycylation."
    Rogowski K., Juge F., van Dijk J., Wloga D., Strub J.-M., Levilliers N., Thomas D., Bre M.-H., Van Dorsselaer A., Gaertig J., Janke C.
    Cell 137:1076-1087(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY.
  4. "TTLL10 is a protein polyglycylase that can modify nucleosome assembly protein 1."
    Ikegami K., Horigome D., Mukai M., Livnat I., MacGregor G.R., Setou M.
    FEBS Lett. 582:1129-1134(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF GLU-499.
  5. "TTLL10 can perform tubulin glycylation when co-expressed with TTLL8."
    Ikegami K., Setou M.
    FEBS Lett. 583:1957-1963(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiTTL10_MOUSE
AccessioniPrimary (citable) accession number: A4Q9F3
Secondary accession number(s): Q3V0K4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: May 15, 2007
Last modified: June 8, 2016
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.