A4PES0 (WEE2_PIG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Wee1-like protein kinase 2
EC=2.7.10.2

Alternative name(s):
Wee1-like protein kinase 1B
Wee1B kinase
Short name=pWee1B
Short name=pigWee1B

Gene names

Name:	WEE2
Synonyms:	WEE1B

Organism

Sus scrofa (Pig) [Reference proteome]

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	565 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Oocyte-specific protein tyrosine kinase that phosphorylates and inhibits CDK1 and acts as a key regulator of meiosis during both prophase I and metaphase II. Required to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage, a long period of quiescence at dictyate prophase I, by phosphorylating CDK1 at 'Tyr-15', leading to inhibit CDK1 activity and prevent meiotic reentry. Also required for metaphase II exit during egg activation by phosphorylating CDK1 at 'Tyr-15', to ensure exit from meiosis in oocytes and promote pronuclear formation. Ref.1
Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Subcellular location	Nucleus Ref.3.
Tissue specificity	Ovary-specific. Ref.1
Developmental stage	Detected only in the oocytes throughout oocyte maturation period. Ref.1
Post-translational modification	Phosphorylation leads to increase its activity.
Miscellaneous	Continuous activation of WEE2 is a cause of meiotic failure of small oocytes. In mammals, oocytes with a diameter less than 80% of that of full-grown oocytes cannot start meiotic maturation (Ref.2).
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily. Contains 1 protein kinase domain.
Sequence caution	The sequence BAF56108.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Biological process	Meiosis
Cellular component	Nucleus
Domain	Coiled coil
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase Tyrosine-protein kinase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	egg activation Inferred from electronic annotation. Source: Compara female meiosis Inferred from direct assay Ref.1Ref.3. Source: UniProtKB female pronucleus assembly Inferred from electronic annotation. Source: Compara meiotic metaphase II Inferred from electronic annotation. Source: Compara meiotic prophase I Inferred from direct assay Ref.1Ref.3. Source: UniProtKB mitosis Inferred from electronic annotation. Source: InterPro negative regulation of oocyte development Inferred from direct assay Ref.1Ref.3. Source: UniProtKB regulation of meiosis I Inferred from direct assay Ref.1Ref.3. Source: UniProtKB
Cellular_component	centrosome Inferred from electronic annotation. Source: Compara cytoplasm Inferred from electronic annotation. Source: Compara nucleus Inferred from direct assay Ref.3. Source: UniProtKB
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: InterPro non-membrane spanning protein tyrosine kinase activity Inferred from electronic annotation. Source: EC protein serine/threonine kinase activity Inferred from electronic annotation. Source: InterPro protein tyrosine kinase activity Traceable author statement Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 565	565	Wee1-like protein kinase 2		PRO_0000409526
Regions
	Domain	214 – 492	279	Protein kinase
	Nucleotide binding	220 – 228	9	ATP By similarity
	Coiled coil	495 – 521	27	Potential
	Motif	173 – 175	3	Nuclear localization signal
	Motif	317 – 331	15	Nuclear export signal By similarity
Sites
	Active site	341	1	Proton acceptor By similarity
	Metal binding	346	1	Magnesium; via carbonyl oxygen By similarity
	Metal binding	382	1	Magnesium; via carbonyl oxygen By similarity
	Binding site	243	1	ATP By similarity
Amino acid modifications
	Modified residue	77	1	Phosphoserine Ref.3
Experimental info
	Mutagenesis	77	1	S → A: Abolishes phosphorylation and ability to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage. Ref.3
	Mutagenesis	118	1	S → A: Does not affect phosphorylation. Ref.3
	Mutagenesis	133	1	S → A: Does not affect phosphorylation. Ref.3
	Mutagenesis	149	1	S → A: Does not affect phosphorylation. Ref.3
	Mutagenesis	174 – 175	2	RK → TT: Abolishes nuclear localization.

Sequences

	Sequence		Length	Mass (Da)
	A4PES0 [UniParc]. Last modified May 31, 2011. Version 2. Checksum: 2855273D6B1548BC	FASTA	565	62,928
	10 20 30 40 50 60 MGDNGDNKEL KQKLNFSYSE EEQEDEGQKE AQESKKVQYH TPERCGHQDS EAKFTPPRTP 70 80 90 100 110 120 LNHVCELSTP QVKDRASPDQ GLRTPVSRPH TRPETPAPPD KSKPPPHCES PFTPRGHSSQ 130 140 150 160 170 180 SVISTGKLPS RGSKHLRLTP GPLTDEMTSL ALVNINPFTP ESYRRQFLKS NGKRKTRRDL 190 200 210 220 230 240 EEAGPEEGKV EKGLPAKRCV LRETNMACRY EKEFLEVEKI GVGEFGTVYK CIKRLDGCVY 250 260 270 280 290 300 AIKRSTKPVS GLSDENLAMH EVYAHSVLGH HPHVVRYYSS WAEDDHMMIQ NEYCNGGSLQ 310 320 330 340 350 360 AAISENAKSG NHFQEPKLKD ILLQISLGLK YIHNYGMVHM DIKPSNIFIC HKIPSDSPVV 370 380 390 400 410 420 PEEAENEADW FLSANVTYKI GDLGHVTSIS EPQVEEGDSR FLAKEILQEN YQHLPKADIF 430 440 450 460 470 480 ALGLTIAVAA GAEALPTNGT SWHHIREGQL PNIPQDLSKE FYNLLKDMID PDPVARPSAA 490 500 510 520 530 540 ALTRSRVLCP SLGRTEELQQ QLNLEKFKTA TLERELKEVQ RAQSSKEGQS SPGVTGTHTG 550 560 SRSTRRLVGG KSAKSSSFTW GQSSP

References

[1]	"Critical effect of pigWee1B on the regulation of meiotic resumption in porcine immature oocytes." Shimaoka T., Nishimura T., Kano K., Naito K. Cell Cycle 8:2375-2384(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[2]	"Insufficient amount of Cdc2 and continuous activation of Wee1 B are the cause of meiotic failure in porcine growing oocytes." Nishimura T., Shimaoka T., Kano K., Naito K. J. Reprod. Dev. 55:553-557(2009) [PubMed] [Europe PMC] [Abstract] Cited for: INVOLVEMENT IN SMALL OOCYTES.
[3]	"Analyses of the regulatory mechanism of porcine WEE1B: the phosphorylation sites of porcine WEE1B and mouse WEE1B are different." Shimaoka T., Nishimura T., Kano K., Naito K. J. Reprod. Dev. 57:223-228(2011) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, PHOSPHORYLATION AT SER-77, MUTAGENESIS OF SER-77; SER-118; SER-133; SER-149 AND 174-ARG-LYS-175.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB276373 mRNA. Translation: BAF56108.1. Different initiation.
RefSeq	NP_001090976.2. NM_001097507.2.
UniGene	Ssc.43347.
3D structure databases
ProteinModelPortal	A4PES0.
ModBase	Search...
Protein-protein interaction databases
STRING	9823.ENSSSCP00000017471.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSSSCT00000017953; ENSSSCP00000017471; ENSSSCG00000016489.
GeneID	100048934.
KEGG	ssc:100048934.
Organism-specific databases
CTD	399355.
Phylogenomic databases
eggNOG	COG0515.
GeneTree	ENSGT00530000063230.
HOGENOM	HOG000004824.
HOVERGEN	HBG005050.
KO	K06632.
OMA	ANVMYKI.
OrthoDB	EOG4FJ88X.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR008271. Ser/Thr_kinase_AS. IPR017164. Wee1-like_protein_kinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
PIRSF	PIRSF037281. Wee1-like_protein_kinase. 1 hit.
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

WEE2_PIG

Accession

Primary (citable) accession number: A4PES0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 31, 2011
Last sequence update:	May 31, 2011
Last modified:	April 3, 2013
This is version 47 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program