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A4PES0 (WEE2_PIG) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Wee1-like protein kinase 2

EC=2.7.10.2
Alternative name(s):
Wee1-like protein kinase 1B
Wee1B kinase
Short name=pWee1B
Short name=pigWee1B
Gene names
Name:WEE2
Synonyms:WEE1B
OrganismSus scrofa (Pig) [Reference proteome]
Taxonomic identifier9823 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus

Protein attributes

Sequence length565 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Oocyte-specific protein tyrosine kinase that phosphorylates and inhibits CDK1 and acts as a key regulator of meiosis during both prophase I and metaphase II. Required to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage, a long period of quiescence at dictyate prophase I, by phosphorylating CDK1 at 'Tyr-15', leading to inhibit CDK1 activity and prevent meiotic reentry. Also required for metaphase II exit during egg activation by phosphorylating CDK1 at 'Tyr-15', to ensure exit from meiosis in oocytes and promote pronuclear formation. Ref.1

Catalytic activity

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Subcellular location

Nucleus Ref.3.

Tissue specificity

Ovary-specific. Ref.1

Developmental stage

Detected only in the oocytes throughout oocyte maturation period. Ref.1

Post-translational modification

Phosphorylation leads to increase its activity.

Miscellaneous

Continuous activation of WEE2 is a cause of meiotic failure of small oocytes. In mammals, oocytes with a diameter less than 80% of that of full-grown oocytes cannot start meiotic maturation (Ref.2).

Sequence similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.

Contains 1 protein kinase domain.

Sequence caution

The sequence BAF56108.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
   Biological processMeiosis
   Cellular componentNucleus
   DomainCoiled coil
   LigandATP-binding
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
Tyrosine-protein kinase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processfemale meiotic division

Inferred from direct assay Ref.1Ref.3. Source: UniProtKB

female pronucleus assembly

Inferred from electronic annotation. Source: Ensembl

meiotic prophase I

Inferred from direct assay Ref.1Ref.3. Source: UniProtKB

mitotic nuclear division

Inferred from electronic annotation. Source: InterPro

negative regulation of cyclin-dependent protein serine/threonine kinase activity

Inferred from electronic annotation. Source: Ensembl

negative regulation of oocyte development

Inferred from direct assay Ref.1Ref.3. Source: UniProtKB

negative regulation of oocyte maturation

Inferred from electronic annotation. Source: Ensembl

peptidyl-tyrosine phosphorylation

Traceable author statement Ref.1. Source: GOC

regulation of meiosis I

Inferred from direct assay Ref.1Ref.3. Source: UniProtKB

   Cellular_componentcentrosome

Inferred from electronic annotation. Source: Ensembl

cytoplasm

Inferred from electronic annotation. Source: Ensembl

nucleus

Inferred from direct assay Ref.3. Source: UniProtKB

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

magnesium ion binding

Inferred from electronic annotation. Source: InterPro

non-membrane spanning protein tyrosine kinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

protein serine/threonine kinase activity

Inferred from electronic annotation. Source: InterPro

protein tyrosine kinase activity

Traceable author statement Ref.1. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 565565Wee1-like protein kinase 2
PRO_0000409526

Regions

Domain214 – 492279Protein kinase
Nucleotide binding220 – 2289ATP By similarity
Coiled coil495 – 52127 Potential
Motif173 – 1753Nuclear localization signal
Motif317 – 33115Nuclear export signal By similarity

Sites

Active site3411Proton acceptor By similarity
Metal binding3461Magnesium; via carbonyl oxygen By similarity
Metal binding3821Magnesium; via carbonyl oxygen By similarity
Binding site2431ATP By similarity

Amino acid modifications

Modified residue771Phosphoserine Ref.3

Experimental info

Mutagenesis771S → A: Abolishes phosphorylation and ability to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage. Ref.3
Mutagenesis1181S → A: Does not affect phosphorylation. Ref.3
Mutagenesis1331S → A: Does not affect phosphorylation. Ref.3
Mutagenesis1491S → A: Does not affect phosphorylation. Ref.3
Mutagenesis174 – 1752RK → TT: Abolishes nuclear localization.

Sequences

Sequence LengthMass (Da)Tools
A4PES0 [UniParc].

Last modified May 31, 2011. Version 2.
Checksum: 2855273D6B1548BC

FASTA56562,928
        10         20         30         40         50         60 
MGDNGDNKEL KQKLNFSYSE EEQEDEGQKE AQESKKVQYH TPERCGHQDS EAKFTPPRTP 

        70         80         90        100        110        120 
LNHVCELSTP QVKDRASPDQ GLRTPVSRPH TRPETPAPPD KSKPPPHCES PFTPRGHSSQ 

       130        140        150        160        170        180 
SVISTGKLPS RGSKHLRLTP GPLTDEMTSL ALVNINPFTP ESYRRQFLKS NGKRKTRRDL 

       190        200        210        220        230        240 
EEAGPEEGKV EKGLPAKRCV LRETNMACRY EKEFLEVEKI GVGEFGTVYK CIKRLDGCVY 

       250        260        270        280        290        300 
AIKRSTKPVS GLSDENLAMH EVYAHSVLGH HPHVVRYYSS WAEDDHMMIQ NEYCNGGSLQ 

       310        320        330        340        350        360 
AAISENAKSG NHFQEPKLKD ILLQISLGLK YIHNYGMVHM DIKPSNIFIC HKIPSDSPVV 

       370        380        390        400        410        420 
PEEAENEADW FLSANVTYKI GDLGHVTSIS EPQVEEGDSR FLAKEILQEN YQHLPKADIF 

       430        440        450        460        470        480 
ALGLTIAVAA GAEALPTNGT SWHHIREGQL PNIPQDLSKE FYNLLKDMID PDPVARPSAA 

       490        500        510        520        530        540 
ALTRSRVLCP SLGRTEELQQ QLNLEKFKTA TLERELKEVQ RAQSSKEGQS SPGVTGTHTG 

       550        560 
SRSTRRLVGG KSAKSSSFTW GQSSP 

« Hide

References

[1]"Critical effect of pigWee1B on the regulation of meiotic resumption in porcine immature oocytes."
Shimaoka T., Nishimura T., Kano K., Naito K.
Cell Cycle 8:2375-2384(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[2]"Insufficient amount of Cdc2 and continuous activation of Wee1 B are the cause of meiotic failure in porcine growing oocytes."
Nishimura T., Shimaoka T., Kano K., Naito K.
J. Reprod. Dev. 55:553-557(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: INVOLVEMENT IN SMALL OOCYTES.
[3]"Analyses of the regulatory mechanism of porcine WEE1B: the phosphorylation sites of porcine WEE1B and mouse WEE1B are different."
Shimaoka T., Nishimura T., Kano K., Naito K.
J. Reprod. Dev. 57:223-228(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, PHOSPHORYLATION AT SER-77, MUTAGENESIS OF SER-77; SER-118; SER-133; SER-149 AND 174-ARG-LYS-175.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB276373 mRNA. Translation: BAF56108.1. Different initiation.
RefSeqNP_001090976.2. NM_001097507.2.
XP_005673170.1. XM_005673113.1.
UniGeneSsc.43347.

3D structure databases

ProteinModelPortalA4PES0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9823.ENSSSCP00000017471.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSSSCT00000017953; ENSSSCP00000017471; ENSSSCG00000016489.
GeneID100048934.
KEGGssc:100048934.

Organism-specific databases

CTD399355.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00530000063230.
HOGENOMHOG000004824.
HOVERGENHBG005050.
KOK06632.
OMAICHKMQS.
OrthoDBEOG7N63M9.
TreeFamTF101088.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR017164. Wee1-like_protein_kinase.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
PIRSFPIRSF037281. Wee1-like_protein_kinase. 1 hit.
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameWEE2_PIG
AccessionPrimary (citable) accession number: A4PES0
Entry history
Integrated into UniProtKB/Swiss-Prot: May 31, 2011
Last sequence update: May 31, 2011
Last modified: July 9, 2014
This is version 55 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families