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A4PES0

- WEE2_PIG

UniProt

A4PES0 - WEE2_PIG

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Protein

Wee1-like protein kinase 2

Gene

WEE2

Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Oocyte-specific protein tyrosine kinase that phosphorylates and inhibits CDK1 and acts as a key regulator of meiosis during both prophase I and metaphase II. Required to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage, a long period of quiescence at dictyate prophase I, by phosphorylating CDK1 at 'Tyr-15', leading to inhibit CDK1 activity and prevent meiotic reentry. Also required for metaphase II exit during egg activation by phosphorylating CDK1 at 'Tyr-15', to ensure exit from meiosis in oocytes and promote pronuclear formation.1 Publication

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei243 – 2431ATPPROSITE-ProRule annotation
Active sitei341 – 3411Proton acceptorPROSITE-ProRule annotation
Metal bindingi346 – 3461Magnesium; via carbonyl oxygenBy similarity
Metal bindingi382 – 3821Magnesium; via carbonyl oxygenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi220 – 2289ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. magnesium ion binding Source: InterPro
  3. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC
  4. protein serine/threonine kinase activity Source: InterPro
  5. protein tyrosine kinase activity Source: UniProtKB

GO - Biological processi

  1. female meiotic division Source: UniProtKB
  2. female pronucleus assembly Source: Ensembl
  3. mitotic nuclear division Source: InterPro
  4. negative regulation of cyclin-dependent protein serine/threonine kinase activity Source: Ensembl
  5. negative regulation of oocyte development Source: UniProtKB
  6. negative regulation of oocyte maturation Source: Ensembl
  7. peptidyl-tyrosine phosphorylation Source: GOC
  8. regulation of meiosis I Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase, Tyrosine-protein kinase

Keywords - Biological processi

Meiosis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Wee1-like protein kinase 2 (EC:2.7.10.2)
Alternative name(s):
Wee1-like protein kinase 1B
Wee1B kinase
Short name:
pWee1B
Short name:
pigWee1B
Gene namesi
Name:WEE2
Synonyms:WEE1B
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
ProteomesiUP000008227: Chromosome 18

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: Ensembl
  2. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi77 – 771S → A: Abolishes phosphorylation and ability to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage. 1 Publication
Mutagenesisi118 – 1181S → A: Does not affect phosphorylation. 1 Publication
Mutagenesisi133 – 1331S → A: Does not affect phosphorylation. 1 Publication
Mutagenesisi149 – 1491S → A: Does not affect phosphorylation. 1 Publication
Mutagenesisi174 – 1752RK → TT: Abolishes nuclear localization. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 565565Wee1-like protein kinase 2PRO_0000409526Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei77 – 771Phosphoserine1 Publication

Post-translational modificationi

Phosphorylation leads to increase its activity.1 Publication

Keywords - PTMi

Phosphoprotein

Expressioni

Tissue specificityi

Ovary-specific.1 Publication

Developmental stagei

Detected only in the oocytes throughout oocyte maturation period.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000017471.

Structurei

3D structure databases

ProteinModelPortaliA4PES0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini214 – 492279Protein kinasePROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili495 – 52127Sequence AnalysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi173 – 1753Nuclear localization signal
Motifi317 – 33115Nuclear export signalBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00530000063230.
HOGENOMiHOG000004824.
HOVERGENiHBG005050.
InParanoidiA4PES0.
KOiK06632.
OMAiICHKMQS.
OrthoDBiEOG7N63M9.
TreeFamiTF101088.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR017164. Wee1-like_protein_kinase.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PIRSFiPIRSF037281. Wee1-like_protein_kinase. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4PES0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGDNGDNKEL KQKLNFSYSE EEQEDEGQKE AQESKKVQYH TPERCGHQDS
60 70 80 90 100
EAKFTPPRTP LNHVCELSTP QVKDRASPDQ GLRTPVSRPH TRPETPAPPD
110 120 130 140 150
KSKPPPHCES PFTPRGHSSQ SVISTGKLPS RGSKHLRLTP GPLTDEMTSL
160 170 180 190 200
ALVNINPFTP ESYRRQFLKS NGKRKTRRDL EEAGPEEGKV EKGLPAKRCV
210 220 230 240 250
LRETNMACRY EKEFLEVEKI GVGEFGTVYK CIKRLDGCVY AIKRSTKPVS
260 270 280 290 300
GLSDENLAMH EVYAHSVLGH HPHVVRYYSS WAEDDHMMIQ NEYCNGGSLQ
310 320 330 340 350
AAISENAKSG NHFQEPKLKD ILLQISLGLK YIHNYGMVHM DIKPSNIFIC
360 370 380 390 400
HKIPSDSPVV PEEAENEADW FLSANVTYKI GDLGHVTSIS EPQVEEGDSR
410 420 430 440 450
FLAKEILQEN YQHLPKADIF ALGLTIAVAA GAEALPTNGT SWHHIREGQL
460 470 480 490 500
PNIPQDLSKE FYNLLKDMID PDPVARPSAA ALTRSRVLCP SLGRTEELQQ
510 520 530 540 550
QLNLEKFKTA TLERELKEVQ RAQSSKEGQS SPGVTGTHTG SRSTRRLVGG
560
KSAKSSSFTW GQSSP
Length:565
Mass (Da):62,928
Last modified:May 31, 2011 - v2
Checksum:i2855273D6B1548BC
GO

Sequence cautioni

The sequence BAF56108.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB276373 mRNA. Translation: BAF56108.1. Different initiation.
RefSeqiNP_001090976.2. NM_001097507.2.
XP_005673170.1. XM_005673113.1.
UniGeneiSsc.43347.

Genome annotation databases

EnsembliENSSSCT00000017953; ENSSSCP00000017471; ENSSSCG00000016489.
GeneIDi100048934.
KEGGissc:100048934.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB276373 mRNA. Translation: BAF56108.1 . Different initiation.
RefSeqi NP_001090976.2. NM_001097507.2.
XP_005673170.1. XM_005673113.1.
UniGenei Ssc.43347.

3D structure databases

ProteinModelPortali A4PES0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9823.ENSSSCP00000017471.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSSSCT00000017953 ; ENSSSCP00000017471 ; ENSSSCG00000016489 .
GeneIDi 100048934.
KEGGi ssc:100048934.

Organism-specific databases

CTDi 399355.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00530000063230.
HOGENOMi HOG000004824.
HOVERGENi HBG005050.
InParanoidi A4PES0.
KOi K06632.
OMAi ICHKMQS.
OrthoDBi EOG7N63M9.
TreeFami TF101088.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR017164. Wee1-like_protein_kinase.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
PIRSFi PIRSF037281. Wee1-like_protein_kinase. 1 hit.
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Critical effect of pigWee1B on the regulation of meiotic resumption in porcine immature oocytes."
    Shimaoka T., Nishimura T., Kano K., Naito K.
    Cell Cycle 8:2375-2384(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  2. "Insufficient amount of Cdc2 and continuous activation of Wee1 B are the cause of meiotic failure in porcine growing oocytes."
    Nishimura T., Shimaoka T., Kano K., Naito K.
    J. Reprod. Dev. 55:553-557(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INVOLVEMENT IN SMALL OOCYTES.
  3. "Analyses of the regulatory mechanism of porcine WEE1B: the phosphorylation sites of porcine WEE1B and mouse WEE1B are different."
    Shimaoka T., Nishimura T., Kano K., Naito K.
    J. Reprod. Dev. 57:223-228(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, PHOSPHORYLATION AT SER-77, MUTAGENESIS OF SER-77; SER-118; SER-133; SER-149 AND 174-ARG-LYS-175.

Entry informationi

Entry nameiWEE2_PIG
AccessioniPrimary (citable) accession number: A4PES0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 31, 2011
Last sequence update: May 31, 2011
Last modified: October 29, 2014
This is version 58 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Continuous activation of WEE2 is a cause of meiotic failure of small oocytes. In mammals, oocytes with a diameter less than 80% of that of full-grown oocytes cannot start meiotic maturation (PubMed:19550110).1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3