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Protein

Wee1-like protein kinase 2

Gene

WEE2

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Oocyte-specific protein tyrosine kinase that phosphorylates and inhibits CDK1 and acts as a key regulator of meiosis during both prophase I and metaphase II. Required to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage, a long period of quiescence at dictyate prophase I, by phosphorylating CDK1 at 'Tyr-15', leading to inhibit CDK1 activity and prevent meiotic reentry. Also required for metaphase II exit during egg activation by phosphorylating CDK1 at 'Tyr-15', to ensure exit from meiosis in oocytes and promote pronuclear formation.1 Publication

Miscellaneous

Continuous activation of WEE2 is a cause of meiotic failure of small oocytes. In mammals, oocytes with a diameter less than 80% of that of full-grown oocytes cannot start meiotic maturation (PubMed:19550110).1 Publication

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei243ATPPROSITE-ProRule annotation1
Active sitei341Proton acceptorPROSITE-ProRule annotation1
Metal bindingi346Magnesium; via carbonyl oxygenBy similarity1
Metal bindingi382Magnesium; via carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi220 – 228ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • female meiotic nuclear division Source: UniProtKB
  • female pronucleus assembly Source: Ensembl
  • mitotic cell cycle Source: InterPro
  • negative regulation of cyclin-dependent protein serine/threonine kinase activity Source: Ensembl
  • negative regulation of meiotic cell cycle process involved in oocyte maturation Source: AgBase
  • negative regulation of oocyte development Source: UniProtKB
  • regulation of meiosis I Source: UniProtKB

Keywordsi

Molecular functionKinase, Transferase, Tyrosine-protein kinase
Biological processMeiosis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Wee1-like protein kinase 2 (EC:2.7.10.2)
Alternative name(s):
Wee1-like protein kinase 1B
Wee1B kinase
Short name:
pWee1B
Short name:
pigWee1B
Gene namesi
Name:WEE2
Synonyms:WEE1B
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Chromosome 18

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi77S → A: Abolishes phosphorylation and ability to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage. 1 Publication1
Mutagenesisi118S → A: Does not affect phosphorylation. 1 Publication1
Mutagenesisi133S → A: Does not affect phosphorylation. 1 Publication1
Mutagenesisi149S → A: Does not affect phosphorylation. 1 Publication1
Mutagenesisi174 – 175RK → TT: Abolishes nuclear localization. 1 Publication2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004095261 – 565Wee1-like protein kinase 2Add BLAST565

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei77Phosphoserine1 Publication1

Post-translational modificationi

Phosphorylation leads to increase its activity.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiA4PES0.

PTM databases

iPTMnetiA4PES0.

Expressioni

Tissue specificityi

Ovary-specific.1 Publication

Developmental stagei

Detected only in the oocytes throughout oocyte maturation period.1 Publication

Gene expression databases

BgeeiENSSSCG00000016489.

Interactioni

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000017471.

Structurei

3D structure databases

ProteinModelPortaliA4PES0.
SMRiA4PES0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini214 – 492Protein kinasePROSITE-ProRule annotationAdd BLAST279

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili495 – 521Sequence analysisAdd BLAST27

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi173 – 175Nuclear localization signal3
Motifi317 – 331Nuclear export signalBy similarityAdd BLAST15

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0601. Eukaryota.
ENOG410XS1M. LUCA.
GeneTreeiENSGT00530000063230.
HOGENOMiHOG000004824.
HOVERGENiHBG005050.
InParanoidiA4PES0.
KOiK06632.
OMAiDWFLSAN.
OrthoDBiEOG091G049H.
TreeFamiTF101088.

Family and domain databases

InterProiView protein in InterPro
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR017164. Wee1-like_protein_kinase.
PfamiView protein in Pfam
PF00069. Pkinase. 1 hit.
PIRSFiPIRSF037281. Wee1-like_protein_kinase. 1 hit.
SMARTiView protein in SMART
SM00220. S_TKc. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.

Sequencei

Sequence statusi: Complete.

A4PES0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDNGDNKEL KQKLNFSYSE EEQEDEGQKE AQESKKVQYH TPERCGHQDS
60 70 80 90 100
EAKFTPPRTP LNHVCELSTP QVKDRASPDQ GLRTPVSRPH TRPETPAPPD
110 120 130 140 150
KSKPPPHCES PFTPRGHSSQ SVISTGKLPS RGSKHLRLTP GPLTDEMTSL
160 170 180 190 200
ALVNINPFTP ESYRRQFLKS NGKRKTRRDL EEAGPEEGKV EKGLPAKRCV
210 220 230 240 250
LRETNMACRY EKEFLEVEKI GVGEFGTVYK CIKRLDGCVY AIKRSTKPVS
260 270 280 290 300
GLSDENLAMH EVYAHSVLGH HPHVVRYYSS WAEDDHMMIQ NEYCNGGSLQ
310 320 330 340 350
AAISENAKSG NHFQEPKLKD ILLQISLGLK YIHNYGMVHM DIKPSNIFIC
360 370 380 390 400
HKIPSDSPVV PEEAENEADW FLSANVTYKI GDLGHVTSIS EPQVEEGDSR
410 420 430 440 450
FLAKEILQEN YQHLPKADIF ALGLTIAVAA GAEALPTNGT SWHHIREGQL
460 470 480 490 500
PNIPQDLSKE FYNLLKDMID PDPVARPSAA ALTRSRVLCP SLGRTEELQQ
510 520 530 540 550
QLNLEKFKTA TLERELKEVQ RAQSSKEGQS SPGVTGTHTG SRSTRRLVGG
560
KSAKSSSFTW GQSSP
Length:565
Mass (Da):62,928
Last modified:May 31, 2011 - v2
Checksum:i2855273D6B1548BC
GO

Sequence cautioni

The sequence BAF56108 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB276373 mRNA. Translation: BAF56108.1. Different initiation.
RefSeqiNP_001090976.2. NM_001097507.2.
XP_005673170.1. XM_005673113.2.
UniGeneiSsc.43347.

Genome annotation databases

EnsembliENSSSCT00000017953; ENSSSCP00000017471; ENSSSCG00000016489.
GeneIDi100048934.
KEGGissc:100048934.

Similar proteinsi

Entry informationi

Entry nameiWEE2_PIG
AccessioniPrimary (citable) accession number: A4PES0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 31, 2011
Last sequence update: May 31, 2011
Last modified: August 30, 2017
This is version 73 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families