A4PCD4 (TDT_EULMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA nucleotidylexotransferase
EC=2.7.7.31

Alternative name(s):
Terminal addition enzyme
Terminal deoxynucleotidyltransferase
Short name=Terminal transferase

Gene names

Name:

DNTT

Organism

Eulemur macaco (Black lemur) (Petterus macaco)

Taxonomic identifier

30602 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Strepsirrhini › Lemuriformes › Lemuridae › Eulemur

Protein attributes

Sequence length	511 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells By similarity.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
Cofactor	Magnesium By similarity.
Subunit structure	Interacts with PRP19 and DNTTIP1. Forms a ternary complex with DNTTIP2 and core histone. Released from this complex by PCNA By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the DNA polymerase type-X family. Contains 1 BRCT domain.

Ontologies

Keywords
Biological process	Terminal addition
Cellular component	Nucleus
Ligand	Magnesium Metal-binding
Molecular function	Nucleotidyltransferase Transferase
Gene Ontology (GO)
Biological_process	DNA modification Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro DNA nucleotidylexotransferase activity Inferred from electronic annotation. Source: UniProtKB-KW DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 511	511	DNA nucleotidylexotransferase		PRO_0000288558
Regions
	Domain	27 – 124	98	BRCT
	Region	151 – 511	361	Mediates interaction with DNTTIP2 By similarity
	Region	336 – 345	10	Involved in ssDNA binding By similarity
Sites
	Metal binding	253	1	Sodium; via carbonyl oxygen By similarity
	Metal binding	255	1	Sodium; via carbonyl oxygen By similarity
	Metal binding	343	1	Magnesium By similarity
	Metal binding	345	1	Magnesium By similarity
	Metal binding	435	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4PCD4 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: AD54690A90E31E56	FASTA	511	58,585
	10 20 30 40 50 60 MDPLQTAHAG PRKKRPRQTG ASMASTPQDV RFQDLVLFIL EKKMGSTRRT FLTELARRKG 70 80 90 100 110 120 FRVENEFSDS VTHIVAENNS GSDVLEWLQV QKIKASSQLE VLDVSWLIEC MRTGKPVETT 130 140 150 160 170 180 GKHQLVARSD CSASPNPGPQ KTPPLAVQKI SQYACQRRTT LNNCNHVFTD AFEILAENYE 190 200 210 220 230 240 FKENEGCAVT FLRAASVLKS LPFTIITMRD TEGIPCLEDK VKCIIEEIIE DGESSEVKAV 250 260 270 280 290 300 LNDERYQSFK LFTSVFGVGL KTSEKWFRMG FRTLSKIRSD KSLTFTRMQR AGFHYYEDLV 310 320 330 340 350 360 SCVTRAEAEA VSVLVKEAVW AFLPDAFVTM TGGFRRGKKI GHDVDFLITS PGSTEEEEEQ 370 380 390 400 410 420 LLHKVMNLWE KKGLLLYCDL VESTFEKLKL PSRKVDALDH FQKCFLILKL HHQRVVDSQK 430 440 450 460 470 480 SSQQDGKTWK AIRVDLVMCP YERRAFALLG WTGSRQFERD LRRYATHERR MMLDNHGLWD 490 500 510 KTKRIFLKAE SEEEIFAHLG LDYIEPSERN A

References

[1]	"Analysis of a phylogenetically conserved oligodendrocyte-enhancer in a mammalian gene, Opalin." Aruga J., Toyoda A., Yoshikawa F., Nozaki Y., Sakaki Y., Furuichi T. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB265806 Genomic DNA. Translation: BAF51669.1.
3D structure databases
ProteinModelPortal	A4PCD4.
SMR	A4PCD4. Positions 21-129, 148-511.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG003670.
Family and domain databases
InterPro	IPR001357. BRCT_dom. IPR002054. DNA-dir_DNA_pol_X. IPR019843. DNA_pol-X_BS. IPR010996. DNA_pol_b-like_N. IPR018944. DNA_pol_lambd_fingers_domain. IPR022312. DNA_pol_X. IPR002934. Nucleotidyltransferase. IPR027292. TdT. IPR001726. TdT/Mu. [Graphical view]
PANTHER	PTHR11276:SF6. PTHR11276:SF6. 1 hit.
Pfam	PF00533. BRCT. 1 hit. PF10391. DNA_pol_lambd_f. 1 hit. PF01909. NTP_transf_2. 1 hit. [Graphical view]
PIRSF	PIRSF000817. DNA_NT. 1 hit. PIRSF501175. TDT. 1 hit.
PRINTS	PR00869. DNAPOLX. PR00871. DNAPOLXTDT.
SMART	SM00292. BRCT. 1 hit. SM00483. POLXc. 1 hit. [Graphical view]
SUPFAM	SSF52113. BRCT. 1 hit. SSF81585. DNA_pol_lambd_fingers_domain. 1 hit. SSF47802. DNApol_B_N_like. 1 hit.
PROSITE	PS50172. BRCT. 1 hit. PS00522. DNA_POLYMERASE_X. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TDT_EULMA

Accession

Primary (citable) accession number: A4PCD4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	May 15, 2007
Last modified:	May 1, 2013
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program