Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Homeodomain-interacting protein kinase 1

Gene

Hipk1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Serine/threonine-protein kinase involved in transcription regulation and TNF-mediated cellular apoptosis. Plays a role as a corepressor for homeodomain transcription factors. Phosphorylates DAXX and MYB. Phosphorylates DAXX in response to stress, and mediates its translocation from the nucleus to the cytoplasm. Inactivates MYB transcription factor activity by phosphorylation. Prevents MAP3K5-JNK activation in the absence of TNF. TNF triggers its translocation to the cytoplasm in response to stress stimuli, thus activating nuclear MAP3K5-JNK by derepression and promoting apoptosis. May be involved in anti-oxidative stress responses. Involved in the regulation of eye size, lens formation and retinal lamination during late embryogenesis. Promotes angiogenesis and to be involved in erythroid differentiation. May be involved in malignant squamous cell tumor formation (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei219 – 2191ATPPROSITE-ProRule annotation
Active sitei315 – 3151Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi196 – 2049ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Homeodomain-interacting protein kinase 1 (EC:2.7.11.1)
Gene namesi
Name:Hipk1Imported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1304941. Hipk1.

Subcellular locationi

  • Nucleus By similarity
  • Cytoplasm By similarity

  • Note: Predominantly nuclear. Translocates from nucleus to cytoplasm in response to stress stimuli via SENP1-mediated desumoylation (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12111211Homeodomain-interacting protein kinase 1PRO_0000296298Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki25 – 25Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternateBy similarity
Cross-linki25 – 25Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei872 – 8721PhosphoserineBy similarity
Modified residuei1201 – 12011PhosphoserineBy similarity
Cross-linki1204 – 1204Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity

Post-translational modificationi

Phosphorylated and activated by JNK1. Autophosphorylated (By similarity).By similarity
Sumoylated. When conjugated it is directed to nuclear speckles. SENP1-mediated desumoylation is mediated by TNF in response to stress stimuli, triggering transient translocation from nucleus to cytoplasm (By similarity).By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiA4L9P5.
PeptideAtlasiA4L9P5.

PTM databases

iPTMnetiA4L9P5.
PhosphoSiteiA4L9P5.

Interactioni

Subunit structurei

Interacts with Nkx1-2, Nkx2-5, MYB, PARK7, DAXX and p53/TP53. Part of a cytoplasmic complex made of HIPK1, DAB2IP and MAP3K5 in response to TNF. This complex formation promotes MAP3K5-JNK activation and subsequent apoptosis (By similarity).By similarity

Protein-protein interaction databases

IntActiA4L9P5. 1 interaction.
STRINGi10116.ENSRNOP00000061079.

Structurei

3D structure databases

ProteinModelPortaliA4L9P5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini190 – 518329Protein kinasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni885 – 1094210Interaction with TP53By similarityAdd
BLAST
Regioni891 – 998108Required for localization to nuclear specklesBy similarityAdd
BLAST
Regioni902 – 92625SUMO interaction motifs (SIM); required for nuclear localization and kinase activityBy similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi844 – 8474Nuclear localization signal 1 (NLS1)By similarity

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0667. Eukaryota.
ENOG410XPET. LUCA.
HOGENOMiHOG000231785.
HOVERGENiHBG051908.
InParanoidiA4L9P5.
PhylomeDBiA4L9P5.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: A4L9P5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASQLQVFSP PSVSSSAFCS AKKLKIEPSG WDVSGQSSND KYYTHSKTLP
60 70 80 90 100
ATQGQASSSH QVANFNIPAY DQGLLLQAPA VEHIVVTAAD SSGSAATATF
110 120 130 140 150
QSSQTLTHRS NVSLLEPYQK CGLKRKSEEV DSNGSVQIIE EHPPLMLQNR
160 170 180 190 200
TVVGAAATTT TVTTKSSSSS GEGDYQLVQH EILCSMTNSY EVLEFLGRGT
210 220 230 240 250
FGQVAKCWKR STKEIVAIKI LKNHPSYARQ GQIEVSILSR LSSENADEYN
260 270 280 290 300
FVRSYECFQH KNHTCLVFEM LEQNLYDFLK QNKFSPLPLK YIRPILQQVA
310 320 330 340 350
TALMKLKSLG LIHADLKPEN IMLVDPVRQP YRVKVIDFGS ASHVSKAVCS
360 370 380 390 400
TYLQSRYYRA PEIILGLPFC EAIDMWSLGC VIAELFLGWP LYPGASEYDQ
410 420 430 440 450
IRYISQTQGL PAEYLLSAGT KTTRFFNRDP NLGYPLWRLK TPEEHELETG
460 470 480 490 500
IKSKEARKYI FNCLDDMAQV NMSTDLEGTD MLAEKADRRE YIDLLKKMLT
510 520 530 540 550
IDADKRVTPL KTLNHQFVTM THLLDFPHSN HVKSCFQNME ICKRRVHMYD
560 570 580 590 600
TVSQIKSPFT THVAPSTSTN LTMSFSNQLS TVHNQASVLA SSSTAAAATL
610 620 630 640 650
SLANSDVSLL NYQSALYPPS AAPVPGVAQQ GVSLQPGTTQ ICTQTDPFQQ
660 670 680 690 700
TFIVCPPAFQ TGLQATTKHS GFPVRMDNAV PLVPQAPAAQ PLQIQSGVLT
710 720 730 740 750
QGSCTPLMVA TLHPQVATIT PQYAVPFTLS CAAGRPALVE QTAAVQQAWP
760 770 780 790 800
GGTQQILLPS AWQQLPGVAL HNSVQPTAVI PEAMGSSQQL ADWRNAHSHG
810 820 830 840 850
NQYSTIMQQP SLLTNHVTLA TAQPLNVGVA HVVRQQQSSS LPSRKNKQSA
860 870 880 890 900
PVSSTSSLEV LPSQVYSLVG SSPLRTTSSY NSLVPVQDQH QPIIIPDTPS
910 920 930 940 950
PPVSVITIRS DTDEEEDNKF KPSSSSLKAR SNVISYVTVN DSPDSDSSLS
960 970 980 990 1000
SPYPTDTLSA LRGNSGTLLE GPGRTAADGI GTRTIIVPPL KTQLGDCTGA
1010 1020 1030 1040 1050
TQASGLLSSS KTKPVASVSG QSSGCCITPT GYRAQRGGAS AVQPLNLSQN
1060 1070 1080 1090 1100
QQSSSASTSQ ERSSNPAPRR QQAFVAPLSQ APYAFQHGSP LHSTGHPHLA
1110 1120 1130 1140 1150
PAPAHLPSQP HLYTYAAPTS AAALGSTSSI AHLFSPQGSS RHAAAYTTHP
1160 1170 1180 1190 1200
STLVHQVPVS VGPSLLTSAS VAPAQYQHQF ATQSYIGSSR GSTIYTGYPL
1210
SPTKISQYSY L
Length:1,211
Mass (Da):130,801
Last modified:May 1, 2007 - v1
Checksum:iEB1ABD970F71BDF4
GO
Isoform 2 (identifier: A4L9P5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     552-585: Missing.

Show »
Length:1,177
Mass (Da):127,127
Checksum:i5E3C89BD24BFD934
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei552 – 58534Missing in isoform 2. 1 PublicationVSP_052489Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF460311 mRNA. Translation: ABO47653.1.
EF460312 mRNA. Translation: ABO47654.1.
UniGeneiRn.178915.
Rn.4151.

Genome annotation databases

UCSCiRGD:1304941. rat. [A4L9P5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF460311 mRNA. Translation: ABO47653.1.
EF460312 mRNA. Translation: ABO47654.1.
UniGeneiRn.178915.
Rn.4151.

3D structure databases

ProteinModelPortaliA4L9P5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiA4L9P5. 1 interaction.
STRINGi10116.ENSRNOP00000061079.

PTM databases

iPTMnetiA4L9P5.
PhosphoSiteiA4L9P5.

Proteomic databases

PaxDbiA4L9P5.
PeptideAtlasiA4L9P5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:1304941. rat. [A4L9P5-1]

Organism-specific databases

RGDi1304941. Hipk1.

Phylogenomic databases

eggNOGiKOG0667. Eukaryota.
ENOG410XPET. LUCA.
HOGENOMiHOG000231785.
HOVERGENiHBG051908.
InParanoidiA4L9P5.
PhylomeDBiA4L9P5.

Miscellaneous databases

PROiA4L9P5.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHIPK1_RAT
AccessioniPrimary (citable) accession number: A4L9P5
Secondary accession number(s): A4L9P6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: May 1, 2007
Last modified: July 6, 2016
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.