SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

A4KZ49

- POLG_TRMVU

UniProt

A4KZ49 - POLG_TRMVU

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Genome polyprotein
Gene
N/A
Organism
Triticum mosaic virus (isolate Triticum aestivum/United States/U06-123/2006) (TriMV)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Helper component proteinase: required for aphid transmission and also has proteolytic activity. Only cleaves a Gly-Gly dipeptide at its own C-terminus. Interacts with virions and aphid stylets. Acts as a suppressor of RNA-mediated gene silencing, also known as post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs. May have RNA-binding activity By similarity.
Cytoplasmic inclusion protein: has helicase activity. It may be involved in replication By similarity.
Both 6K peptides are indispensable for virus replication By similarity.
Nuclear inclusion protein A: has RNA-binding and proteolytic activities By similarity.
Nuclear inclusion protein B: an RNA-dependent RNA polymerase that plays an essential role in the virus replication By similarity.
Capsid protein: involved in aphid transmission, cell-to-cell and systemis movement, encapsidation of the viral RNA and in the regulation of viral RNA amplification By similarity.

Catalytic activityi

Hydrolyzes glutaminyl bonds, and activity is further restricted by preferences for the amino acids in P6 - P1' that vary with the species of potyvirus, e.g. Glu-Xaa-Xaa-Tyr-Xaa-Gln-|-(Ser or Gly) for the enzyme from tobacco etch virus. The natural substrate is the viral polyprotein, but other proteins and oligopeptides containing the appropriate consensus sequence are also cleaved.
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Hydrolyzes a Gly-|-Gly bond at its own C-terminus, commonly in the sequence -Tyr-Xaa-Val-Gly-|-Gly, in the processing of the potyviral polyprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei383 – 3842Cleavage; by P1 proteinase Reviewed prediction
Active sitei737 – 7371For helper component proteinase activity By similarity
Active sitei809 – 8091For helper component proteinase activity By similarity
Sitei850 – 8512Cleavage; by HC-pro Reviewed prediction
Sitei1148 – 11492Cleavage; by NIa-pro By similarity
Sitei1204 – 12052Cleavage; by NIa-pro By similarity
Sitei1852 – 18532Cleavage; by NIa-pro By similarity
Sitei1902 – 19032Cleavage; by NIa-pro By similarity
Sitei2095 – 20962Cleavage; by NIa-pro By similarity
Active sitei2140 – 21401For nuclear inclusion protein A activity By similarity
Active sitei2174 – 21741For nuclear inclusion protein A activity By similarity
Active sitei2243 – 22431For nuclear inclusion protein A activity By similarity
Sitei2328 – 23292Cleavage; by NIa-pro By similarity
Sitei2818 – 28192Cleavage; by NIa-pro By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi1291 – 12988ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. RNA binding Source: InterPro
  3. RNA-directed RNA polymerase activity Source: UniProtKB-KW
  4. cysteine-type endopeptidase activity Source: InterPro
  5. helicase activity Source: UniProtKB-KW
  6. structural molecule activity Source: InterPro

GO - Biological processi

  1. RNA-protein covalent cross-linking Source: UniProtKB-KW
  2. transcription, DNA-templated Source: InterPro
  3. viral RNA genome replication Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase, Nucleotidyltransferase, Protease, RNA-directed RNA polymerase, Suppressor of RNA silencing, Thiol protease, Transferase

Keywords - Biological processi

Viral RNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Genome polyprotein
Cleaved into the following 10 chains:
Alternative name(s):
N-terminal protein (EC:3.4.-.-)
Helper component proteinase (EC:3.4.22.45)
Short name:
HC-pro
6 kDa protein 1
Short name:
6K1
6 kDa protein 2
Short name:
6K2
Alternative name(s):
VPg
Nuclear inclusion protein A (EC:3.4.22.44)
Short name:
NI-a
Short name:
NIa
Alternative name(s):
49 kDa proteinase
Short name:
49 kDa-Pro
NIa-pro
Nuclear inclusion protein B (EC:2.7.7.48)
Short name:
NI-b
Short name:
NIb
Alternative name(s):
RNA-directed RNA polymerase
Capsid protein
Short name:
CP
Alternative name(s):
Coat protein
OrganismiTriticum mosaic virus (isolate Triticum aestivum/United States/U06-123/2006) (TriMV)
Taxonomic identifieri1289472 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stagePotyviridaePoacevirus
Virus hostiAvena sativa (Oat) [TaxID: 4498]
Hordeum vulgare (Barley) [TaxID: 4513]
Secale cereale (Rye) [TaxID: 4550]
Triticum aestivum (Wheat) [TaxID: 4565]
x Triticosecale [TaxID: 49317]
ProteomesiUP000008255: Genome

Subcellular locationi

Chain Capsid protein : Virion Reviewed prediction

GO - Cellular componenti

  1. helical viral capsid Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Helical capsid protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 31123112Genome polyprotein
PRO_0000420027Add
BLAST
Chaini1 – 383383P1 proteinase Reviewed prediction
PRO_0000395486Add
BLAST
Chaini384 – 850467Helper component proteinase Reviewed prediction
PRO_0000395487Add
BLAST
Chaini851 – 1148298Protein P3 By similarity
PRO_0000395488Add
BLAST
Chaini1149 – 1204566 kDa protein 1 By similarity
PRO_0000395489Add
BLAST
Chaini1205 – 1852648Cytoplasmic inclusion protein By similarity
PRO_0000395490Add
BLAST
Chaini1853 – 1902506 kDa protein 2 By similarity
PRO_0000395491Add
BLAST
Chaini1903 – 2095193Viral genome-linked protein By similarity
PRO_0000395492Add
BLAST
Chaini2096 – 2328233Nuclear inclusion protein A By similarity
PRO_0000395493Add
BLAST
Chaini2329 – 2818490Nuclear inclusion protein B By similarity
PRO_0000395494Add
BLAST
Chaini2819 – 3112294Capsid protein By similarity
PRO_0000288836Add
BLAST

Post-translational modificationi

VPg is uridylylated by the polymerase and is covalently attached to the 5'-end of the genomic RNA. This uridylylated form acts as a nucleotide-peptide primer for the polymerase By similarity.
Genome polyprotein of potyviruses undergoes post-translational proteolytic processing by the main proteinase NIa-pro resulting in the production of at least ten individual proteins. The P1 proteinase and the HC-pro cleave only their respective C-termini autocatalytically. 6K1 is essential for proper proteolytic separation of P3 from CI By similarity.

Keywords - PTMi

Covalent protein-RNA linkage, Phosphoprotein

Structurei

3D structure databases

ProteinModelPortaliA4KZ49.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1278 – 1429152Helicase ATP-binding
Add
BLAST
Domaini1444 – 1627184Helicase C-terminal
Add
BLAST
Domaini2096 – 2311216Peptidase C4
Add
BLAST
Domaini2569 – 2687119RdRp catalytic
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi1379 – 13824DECH box

Domaini

The N-terminus of helper component proteinase is involved in interaction with stylets. The central part is involved in interaction with virions and the C-terminus is involved in cell-to cell movement of the virus By similarity.

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR025910. P1_Ser_Pept_dom.
IPR001456. Peptidase_C6.
IPR001592. Poty_coat.
IPR001730. Potyv_NIa-pro_dom.
IPR013648. PP_Potyviridae.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00863. Peptidase_C4. 1 hit.
PF00851. Peptidase_C6. 1 hit.
PF13611. Peptidase_S76. 1 hit.
PF00767. Poty_coat. 1 hit.
PF08440. Poty_PP. 1 hit.
PF00680. RdRP_1. 1 hit.
[Graphical view]
PRINTSiPR00966. NIAPOTYPTASE.
SMARTiSM00487. DEXDc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51436. POTYVIRUS_NIA_PRO. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A4KZ49-1 [UniParc]FASTAAdd to Basket

« Hide

MSSKKMMWVP KSAHKAPVVS REPVIRKKEW VARQIPKYIP VSNPSDCRDE     50
ISQTLLHFDS EEAVYDFVWR FPMGSIFWDT NGRIKPVVNC LLRATRMNLD 100
YDVAADVYVC RDCLSCASSY MYFSNYHYDC RELRENHEAV VSCKYEQHIV 150
STFDVFPRYC TQEIEQNVVN WMTETLERYD NEPLRIEKQL QFYNHKTEQM 200
ESRVQEVQVT TAEYAVSDTY VPQQLSRKGS VSAKLTQRRA NKIIMRTHEV 250
ENLIRETIDL CDERQIPITF VDVKHKRCLP RIPLRHMQAK PDISEIVEQG 300
DMYNEVGQFI EQYQNLAEPF RVIRDYEVTR GWSGVILHRD DLALDPQTQA 350
RCLNNLFVVM GRCEHGHLQN ALRPDCLEGL TYYSDTFGKV FNESLVKHHP 400
GKHQFRIGSR TDYEWEELAM WVNAVCPVSF RCADCRPPQS LNEYIENIRM 450
SKAMAELAGR QDALSKTLHK WTTMLISSVL TTEIRARDNL EPIQERIFTR 500
NMPLGPLYDV AGAMNRAVID IQTAVQNMQL SIGNSNMNEQ QRNQTLLNEI 550
NKIKQHSFMQ TKEMLSRFEN IAQTYQNIIS SASQPLSIHS MRQLMMDSRM 600
DESFEFDIMR KKGSIASIAP MAFRTFEDIY SQPGVYNQKW LNLTPSGRFQ 650
TDIDYLRLDL PIDVIQKKKH VVNRNEIKEE TCYVIVGQVN VSFCEVVARC 700
FVPIPHVLRV GSPQNPTMIK IQDQEGGKTL VPKSGFCYVL QLVLMLGYVP 750
DQLTAAFVKD VGIVVESLGP WPLFVDYLGA IKNLIIRYPT TIKAPTALHI 800
VDHVDTVIHV MTTLGCVNKG EHYLTLQSVA QLHDAAMTVN IETFKDYRIG 850
GVVPQLKHML QSEEHMLEVL EAKPQWLVHL LLSPTQIWAL SQSVVKYQVI 900
HKVMTSNPDL AVALAQLVAI SSNFSIFKNT EHVIQKYFEV SKQLQNVSGV 950
ILGEHNEYFE TAFAQYSALR FSTDVVLLMD QFSTRKKTLD DLEDYYRKTI 1000
PSILIECGLL GPSDFGWRKR LVRGVVDRGS GLKSTVKSLG SFSTKEKWIS 1050
WSGLGSGTIT CVKFPFVCLQ RSGSWLYSST KTTAFNAVWM AGIKCVKSNV 1100
RSILLDSALY GAITLALLCA IKLIRKAFRF VEGLIKEDTS DDEDYVLHAK 1150
AASDSLYIQC LAWLALVVGC FNSGLANDIY FSTTKYRTLL DMVKTAHSDS 1200
FVFHAGDEEE GEIVELITRD NFVDYVYNHS DPLMEFDSET LLGWYTRISY 1250
QGRVLEHPLR VGTNCHLTRE NVDEIAKNIA TGAGNEFIVV GDVGSGKSTK 1300
LPIAVSTYGP VLILVPSREL VNNLCSSIWH VGKKQASTYM MNCITRGTSN 1350
ISIMTYGYAL ALFSHCPIEL QKYRFIQMDE CHEFSSHMIT FYSWWRESGK 1400
FTKLFKTTAT PPGTVIKGGC VPTNHKVDVI EIRDVSVEEF CRRSIDSHAE 1450
GLRSLMPNGG RVIMFVPSRR ECELARSSLI SIPGARTWVV YRAAATQATK 1500
LVAELADDKH YFQIIITTTV LQNGVNLDPD CVVDFGQTFE AAYDRDSRQL 1550
GVRRRNINPG ELIQRVGRVG RNKPGKFIQV GKRLEHEVVP NSCCVTDAIL 1600
MSFTLELAPF ISSHLIDEVN FVTREQVRTA MKFSAPLLFM IHYVRRDGRM 1650
LNGYYQQLKG LLLQTSDVAL CDTLVGDAET NSFLTLRQYQ LRGIIEAQEV 1700
LPDLPIPFYS SEFALPFYLE IGQITKEAIR ARSFTLRIKT PDVKKAVMRL 1750
STSATQIDQT IGILRTRLQL TRERLSKFSE LKATAHNLRL TPIFNTCFDM 1800
GAAKSESTLR ASLTAGEELL SALELARTEK SDKALEKLIL DNPVLGDCLV 1850
FHGGPEEYFD QTLFQTSTGL INKYTVGIAC LTVGLGCTIW YYLKKREKYV 1900
MHGKVHTRET GLTTNHLFVP GMKEHIQEWT GGDHEIGNRF GEAYKRRFIG 1950
RQPTEEQKLS KEKWDKREGQ QTSVYKTLYD LDPTKFKYVV VECPDFDLKK 2000
KLNRQEKKQL DTTIVEACRT RMLDKGQHDF KDVERATVYL FNDNGVGHKV 2050
QLTPHNPLAV SRTTTHPVGF PAEAGRLRQT GQAMEMTPEE LEKALDDNYV 2100
PHSRCQIDIS HLHRHLAIVN TGGMSTQCFI TQTMCVAPYH LAMGFKDNTK 2150
LTIYCSNGVY VMPVPKVEKM ENMDLVVFRM PQDFPPLKRC ATIREPKSSD 2200
EVTLITGKRT THGIQLQFSK VVSIDRKSDT VWKYMIDSVP GVCGGMVMCV 2250
EDGCVVGFHS AAAIRNKVSN GSIFTPVTPQ LLDSLQSSEG HLFDWYFNDD 2300
LISWKGVPTN MDPRNFPVSE TISEFIFHND SKGHGTDKYY GENLTIEGRV 2350
LQSFNTRHVV KGLDDAFAEY VNKFGEPPAD TFTHLPSDLS SDAFYKDFMK 2400
YSTPVEVGTV NIENFEKAVQ AVVELLEQQG FEQGEFSPEM DFYKILNSFN 2450
LDTAMGALYQ CKKKDVLPMA SHEQLATWFW NSLENLATGK LGLWKASLKA 2500
ELRPKEKVLE KKTRVFTAAP FDVSFGAKAF VDDFNNKFYA TQAGSNWTVG 2550
INKFNCGWDE LARRFNPDWK FIDADGSRYD SSLTPLLFNA VLRIRQHFLR 2600
ANGFERRMLS NFYTQLVWTP ISTITGQIVK KNKGGPSGQP STVVDNTMML 2650
MIAVEYAKLQ YGVTDLKYVC NGDDLILNAP QGVCETIRAN FSHSFKELGL 2700
TYEFEQEVDS IDQVEYMSHK WIDCGGVLIP KLKPERIVSV LQWNKSLDLA 2750
SQANKINAAW IESFGYGDLS KFIREYANWW GERNGQVGFL CSEEKVASLY 2800
LTNDVTIHTE EHDEFVFHSG ADQSGVVKDQ TGDKAEGSGT KTEDPPNQTT 2850
DPVNNPSNGG NKDAPQNLNA TVVTKSYTYI PPIMKSLVTI DTAKKMADYT 2900
PPDALISTQA CTLEQFGRWA NAAANGLGLS MQAFQTDVVP YWIYWCIVNS 2950
ASDEHKKLSS WTKVNMTIDD ATGQINLNEG EAQTIYEMSP MFDEAKPTLR 3000
AVMRHFGALA YRWVKFSIAK RKPIIPHNAI KAGLMDVTYF PCCIDFVTVD 3050
QLSPQEQNVR NQVINARVSD TPRALFKHAQ RAGAGEEDTN LRRDDDANYG 3100
RTRVGGAMFG TR 3112
Length:3,112
Mass (Da):352,841
Last modified:July 13, 2010 - v3
Checksum:i5B066BBD6C5D5151
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti2819 – 28191S → E AA sequence 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
FJ263671 Genomic RNA. Translation: ABO41208.2.
RefSeqiYP_002956073.1. NC_012799.1.

Genome annotation databases

GeneIDi7984336.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
FJ263671 Genomic RNA. Translation: ABO41208.2 .
RefSeqi YP_002956073.1. NC_012799.1.

3D structure databases

ProteinModelPortali A4KZ49.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 7984336.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
InterProi IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR025910. P1_Ser_Pept_dom.
IPR001456. Peptidase_C6.
IPR001592. Poty_coat.
IPR001730. Potyv_NIa-pro_dom.
IPR013648. PP_Potyviridae.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00863. Peptidase_C4. 1 hit.
PF00851. Peptidase_C6. 1 hit.
PF13611. Peptidase_S76. 1 hit.
PF00767. Poty_coat. 1 hit.
PF08440. Poty_PP. 1 hit.
PF00680. RdRP_1. 1 hit.
[Graphical view ]
PRINTSi PR00966. NIAPOTYPTASE.
SMARTi SM00487. DEXDc. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEi PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51436. POTYVIRUS_NIA_PRO. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The complete genome sequence of Triticum mosaic virus, a new wheat-infecting virus of the High Plains."
    Fellers J.P., Seifers D., Ryba-White M., Martin T.J.
    Arch. Virol. 154:1511-1515(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Triticum mosaic virus: A new virus isolated from wheat in Kansas."
    Seifers D.L., Martin T.J., Harvey T.L., Fellers J.P., Stack J.P., Ryba-White M., Haber S., Krokhin O.V., Spicer V., Lovat N., Yamchuk A., Standing K.G.
    Plant Dis. 92:808-817(2008)
    Cited for: PROTEIN SEQUENCE OF 2819-2918; 2953-2968; 2988-3000; 3005-3012 AND 3016-3112.

Entry informationi

Entry nameiPOLG_TRMVU
AccessioniPrimary (citable) accession number: A4KZ49
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: July 13, 2010
Last modified: September 3, 2014
This is version 44 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi