Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

A4KZ49

- POLG_TRMVU

UniProt

A4KZ49 - POLG_TRMVU

Protein

Genome polyprotein

Gene
N/A
Organism
Triticum mosaic virus (isolate Triticum aestivum/United States/U06-123/2006) (TriMV)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 45 (01 Oct 2014)
      Sequence version 3 (13 Jul 2010)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Helper component proteinase: required for aphid transmission and also has proteolytic activity. Only cleaves a Gly-Gly dipeptide at its own C-terminus. Interacts with virions and aphid stylets. Acts as a suppressor of RNA-mediated gene silencing, also known as post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs. May have RNA-binding activity By similarity.By similarity
    Cytoplasmic inclusion protein: has helicase activity. It may be involved in replication By similarity.By similarity
    Both 6K peptides are indispensable for virus replication.By similarity
    Nuclear inclusion protein A: has RNA-binding and proteolytic activities.By similarity
    Nuclear inclusion protein B: an RNA-dependent RNA polymerase that plays an essential role in the virus replication.By similarity
    Capsid protein: involved in aphid transmission, cell-to-cell and systemis movement, encapsidation of the viral RNA and in the regulation of viral RNA amplification.By similarity

    Catalytic activityi

    Hydrolyzes glutaminyl bonds, and activity is further restricted by preferences for the amino acids in P6 - P1' that vary with the species of potyvirus, e.g. Glu-Xaa-Xaa-Tyr-Xaa-Gln-|-(Ser or Gly) for the enzyme from tobacco etch virus. The natural substrate is the viral polyprotein, but other proteins and oligopeptides containing the appropriate consensus sequence are also cleaved.
    Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation
    Hydrolyzes a Gly-|-Gly bond at its own C-terminus, commonly in the sequence -Tyr-Xaa-Val-Gly-|-Gly, in the processing of the potyviral polyprotein.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei383 – 3842Cleavage; by P1 proteinaseSequence Analysis
    Active sitei737 – 7371For helper component proteinase activityBy similarity
    Active sitei809 – 8091For helper component proteinase activityBy similarity
    Sitei850 – 8512Cleavage; by HC-proSequence Analysis
    Sitei1148 – 11492Cleavage; by NIa-proBy similarity
    Sitei1204 – 12052Cleavage; by NIa-proBy similarity
    Sitei1852 – 18532Cleavage; by NIa-proBy similarity
    Sitei1902 – 19032Cleavage; by NIa-proBy similarity
    Sitei2095 – 20962Cleavage; by NIa-proBy similarity
    Active sitei2140 – 21401For nuclear inclusion protein A activityPROSITE-ProRule annotation
    Active sitei2174 – 21741For nuclear inclusion protein A activityPROSITE-ProRule annotation
    Active sitei2243 – 22431For nuclear inclusion protein A activityPROSITE-ProRule annotation
    Sitei2328 – 23292Cleavage; by NIa-proBy similarity
    Sitei2818 – 28192Cleavage; by NIa-proBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi1291 – 12988ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cysteine-type endopeptidase activity Source: InterPro
    3. helicase activity Source: UniProtKB-KW
    4. RNA binding Source: InterPro
    5. RNA-directed RNA polymerase activity Source: UniProtKB-KW
    6. structural molecule activity Source: InterPro

    GO - Biological processi

    1. RNA-protein covalent cross-linking Source: UniProtKB-KW
    2. transcription, DNA-templated Source: InterPro
    3. viral RNA genome replication Source: InterPro

    Keywords - Molecular functioni

    Helicase, Hydrolase, Nucleotidyltransferase, Protease, RNA-directed RNA polymerase, Suppressor of RNA silencing, Thiol protease, Transferase

    Keywords - Biological processi

    Viral RNA replication

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Genome polyprotein
    Cleaved into the following 10 chains:
    Alternative name(s):
    N-terminal protein (EC:3.4.-.-)
    Helper component proteinase (EC:3.4.22.45)
    Short name:
    HC-pro
    6 kDa protein 1
    Short name:
    6K1
    6 kDa protein 2
    Short name:
    6K2
    Alternative name(s):
    VPg
    Nuclear inclusion protein A (EC:3.4.22.44)
    Short name:
    NI-a
    Short name:
    NIa
    Alternative name(s):
    49 kDa proteinase
    Short name:
    49 kDa-Pro
    NIa-pro
    Nuclear inclusion protein B (EC:2.7.7.48)
    Short name:
    NI-b
    Short name:
    NIb
    Alternative name(s):
    RNA-directed RNA polymerase
    Capsid protein
    Short name:
    CP
    Alternative name(s):
    Coat protein
    OrganismiTriticum mosaic virus (isolate Triticum aestivum/United States/U06-123/2006) (TriMV)
    Taxonomic identifieri1289472 [NCBI]
    Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stagePotyviridaePoacevirus
    Virus hostiAvena sativa (Oat) [TaxID: 4498]
    Hordeum vulgare (Barley) [TaxID: 4513]
    Secale cereale (Rye) [TaxID: 4550]
    Triticum aestivum (Wheat) [TaxID: 4565]
    x Triticosecale [TaxID: 49317]
    ProteomesiUP000008255: Genome

    Subcellular locationi

    GO - Cellular componenti

    1. helical viral capsid Source: UniProtKB-KW

    Keywords - Cellular componenti

    Capsid protein, Helical capsid protein, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 31123112Genome polyproteinPRO_0000420027Add
    BLAST
    Chaini1 – 383383P1 proteinaseSequence AnalysisPRO_0000395486Add
    BLAST
    Chaini384 – 850467Helper component proteinaseSequence AnalysisPRO_0000395487Add
    BLAST
    Chaini851 – 1148298Protein P3By similarityPRO_0000395488Add
    BLAST
    Chaini1149 – 1204566 kDa protein 1By similarityPRO_0000395489Add
    BLAST
    Chaini1205 – 1852648Cytoplasmic inclusion proteinBy similarityPRO_0000395490Add
    BLAST
    Chaini1853 – 1902506 kDa protein 2By similarityPRO_0000395491Add
    BLAST
    Chaini1903 – 2095193Viral genome-linked proteinBy similarityPRO_0000395492Add
    BLAST
    Chaini2096 – 2328233Nuclear inclusion protein ABy similarityPRO_0000395493Add
    BLAST
    Chaini2329 – 2818490Nuclear inclusion protein BBy similarityPRO_0000395494Add
    BLAST
    Chaini2819 – 3112294Capsid proteinBy similarityPRO_0000288836Add
    BLAST

    Post-translational modificationi

    VPg is uridylylated by the polymerase and is covalently attached to the 5'-end of the genomic RNA. This uridylylated form acts as a nucleotide-peptide primer for the polymerase By similarity.By similarity
    Genome polyprotein of potyviruses undergoes post-translational proteolytic processing by the main proteinase NIa-pro resulting in the production of at least ten individual proteins. The P1 proteinase and the HC-pro cleave only their respective C-termini autocatalytically. 6K1 is essential for proper proteolytic separation of P3 from CI By similarity.By similarity

    Keywords - PTMi

    Covalent protein-RNA linkage, Phosphoprotein

    Structurei

    3D structure databases

    ProteinModelPortaliA4KZ49.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1278 – 1429152Helicase ATP-bindingPROSITE-ProRule annotationAdd
    BLAST
    Domaini1444 – 1627184Helicase C-terminalPROSITE-ProRule annotationAdd
    BLAST
    Domaini2096 – 2311216Peptidase C4PROSITE-ProRule annotationAdd
    BLAST
    Domaini2569 – 2687119RdRp catalyticPROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi1379 – 13824DECH box

    Domaini

    The N-terminus of helper component proteinase is involved in interaction with stylets. The central part is involved in interaction with virions and the C-terminus is involved in cell-to cell movement of the virus By similarity.By similarity

    Sequence similaritiesi

    Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
    Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation
    Contains 1 peptidase C4 domain.PROSITE-ProRule annotation
    Contains 1 peptidase C6 domain.Curated
    Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    InterProiIPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    IPR025910. P1_Ser_Pept_dom.
    IPR001456. Peptidase_C6.
    IPR001592. Poty_coat.
    IPR001730. Potyv_NIa-pro_dom.
    IPR013648. PP_Potyviridae.
    IPR001205. RNA-dir_pol_C.
    IPR007094. RNA-dir_pol_PSvirus.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00270. DEAD. 1 hit.
    PF00271. Helicase_C. 1 hit.
    PF00863. Peptidase_C4. 1 hit.
    PF00851. Peptidase_C6. 1 hit.
    PF13611. Peptidase_S76. 1 hit.
    PF00767. Poty_coat. 1 hit.
    PF08440. Poty_PP. 1 hit.
    PF00680. RdRP_1. 1 hit.
    [Graphical view]
    PRINTSiPR00966. NIAPOTYPTASE.
    SMARTiSM00487. DEXDc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    SSF52540. SSF52540. 1 hit.
    PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    PS51436. POTYVIRUS_NIA_PRO. 1 hit.
    PS50507. RDRP_SSRNA_POS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    A4KZ49-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSKKMMWVP KSAHKAPVVS REPVIRKKEW VARQIPKYIP VSNPSDCRDE     50
    ISQTLLHFDS EEAVYDFVWR FPMGSIFWDT NGRIKPVVNC LLRATRMNLD 100
    YDVAADVYVC RDCLSCASSY MYFSNYHYDC RELRENHEAV VSCKYEQHIV 150
    STFDVFPRYC TQEIEQNVVN WMTETLERYD NEPLRIEKQL QFYNHKTEQM 200
    ESRVQEVQVT TAEYAVSDTY VPQQLSRKGS VSAKLTQRRA NKIIMRTHEV 250
    ENLIRETIDL CDERQIPITF VDVKHKRCLP RIPLRHMQAK PDISEIVEQG 300
    DMYNEVGQFI EQYQNLAEPF RVIRDYEVTR GWSGVILHRD DLALDPQTQA 350
    RCLNNLFVVM GRCEHGHLQN ALRPDCLEGL TYYSDTFGKV FNESLVKHHP 400
    GKHQFRIGSR TDYEWEELAM WVNAVCPVSF RCADCRPPQS LNEYIENIRM 450
    SKAMAELAGR QDALSKTLHK WTTMLISSVL TTEIRARDNL EPIQERIFTR 500
    NMPLGPLYDV AGAMNRAVID IQTAVQNMQL SIGNSNMNEQ QRNQTLLNEI 550
    NKIKQHSFMQ TKEMLSRFEN IAQTYQNIIS SASQPLSIHS MRQLMMDSRM 600
    DESFEFDIMR KKGSIASIAP MAFRTFEDIY SQPGVYNQKW LNLTPSGRFQ 650
    TDIDYLRLDL PIDVIQKKKH VVNRNEIKEE TCYVIVGQVN VSFCEVVARC 700
    FVPIPHVLRV GSPQNPTMIK IQDQEGGKTL VPKSGFCYVL QLVLMLGYVP 750
    DQLTAAFVKD VGIVVESLGP WPLFVDYLGA IKNLIIRYPT TIKAPTALHI 800
    VDHVDTVIHV MTTLGCVNKG EHYLTLQSVA QLHDAAMTVN IETFKDYRIG 850
    GVVPQLKHML QSEEHMLEVL EAKPQWLVHL LLSPTQIWAL SQSVVKYQVI 900
    HKVMTSNPDL AVALAQLVAI SSNFSIFKNT EHVIQKYFEV SKQLQNVSGV 950
    ILGEHNEYFE TAFAQYSALR FSTDVVLLMD QFSTRKKTLD DLEDYYRKTI 1000
    PSILIECGLL GPSDFGWRKR LVRGVVDRGS GLKSTVKSLG SFSTKEKWIS 1050
    WSGLGSGTIT CVKFPFVCLQ RSGSWLYSST KTTAFNAVWM AGIKCVKSNV 1100
    RSILLDSALY GAITLALLCA IKLIRKAFRF VEGLIKEDTS DDEDYVLHAK 1150
    AASDSLYIQC LAWLALVVGC FNSGLANDIY FSTTKYRTLL DMVKTAHSDS 1200
    FVFHAGDEEE GEIVELITRD NFVDYVYNHS DPLMEFDSET LLGWYTRISY 1250
    QGRVLEHPLR VGTNCHLTRE NVDEIAKNIA TGAGNEFIVV GDVGSGKSTK 1300
    LPIAVSTYGP VLILVPSREL VNNLCSSIWH VGKKQASTYM MNCITRGTSN 1350
    ISIMTYGYAL ALFSHCPIEL QKYRFIQMDE CHEFSSHMIT FYSWWRESGK 1400
    FTKLFKTTAT PPGTVIKGGC VPTNHKVDVI EIRDVSVEEF CRRSIDSHAE 1450
    GLRSLMPNGG RVIMFVPSRR ECELARSSLI SIPGARTWVV YRAAATQATK 1500
    LVAELADDKH YFQIIITTTV LQNGVNLDPD CVVDFGQTFE AAYDRDSRQL 1550
    GVRRRNINPG ELIQRVGRVG RNKPGKFIQV GKRLEHEVVP NSCCVTDAIL 1600
    MSFTLELAPF ISSHLIDEVN FVTREQVRTA MKFSAPLLFM IHYVRRDGRM 1650
    LNGYYQQLKG LLLQTSDVAL CDTLVGDAET NSFLTLRQYQ LRGIIEAQEV 1700
    LPDLPIPFYS SEFALPFYLE IGQITKEAIR ARSFTLRIKT PDVKKAVMRL 1750
    STSATQIDQT IGILRTRLQL TRERLSKFSE LKATAHNLRL TPIFNTCFDM 1800
    GAAKSESTLR ASLTAGEELL SALELARTEK SDKALEKLIL DNPVLGDCLV 1850
    FHGGPEEYFD QTLFQTSTGL INKYTVGIAC LTVGLGCTIW YYLKKREKYV 1900
    MHGKVHTRET GLTTNHLFVP GMKEHIQEWT GGDHEIGNRF GEAYKRRFIG 1950
    RQPTEEQKLS KEKWDKREGQ QTSVYKTLYD LDPTKFKYVV VECPDFDLKK 2000
    KLNRQEKKQL DTTIVEACRT RMLDKGQHDF KDVERATVYL FNDNGVGHKV 2050
    QLTPHNPLAV SRTTTHPVGF PAEAGRLRQT GQAMEMTPEE LEKALDDNYV 2100
    PHSRCQIDIS HLHRHLAIVN TGGMSTQCFI TQTMCVAPYH LAMGFKDNTK 2150
    LTIYCSNGVY VMPVPKVEKM ENMDLVVFRM PQDFPPLKRC ATIREPKSSD 2200
    EVTLITGKRT THGIQLQFSK VVSIDRKSDT VWKYMIDSVP GVCGGMVMCV 2250
    EDGCVVGFHS AAAIRNKVSN GSIFTPVTPQ LLDSLQSSEG HLFDWYFNDD 2300
    LISWKGVPTN MDPRNFPVSE TISEFIFHND SKGHGTDKYY GENLTIEGRV 2350
    LQSFNTRHVV KGLDDAFAEY VNKFGEPPAD TFTHLPSDLS SDAFYKDFMK 2400
    YSTPVEVGTV NIENFEKAVQ AVVELLEQQG FEQGEFSPEM DFYKILNSFN 2450
    LDTAMGALYQ CKKKDVLPMA SHEQLATWFW NSLENLATGK LGLWKASLKA 2500
    ELRPKEKVLE KKTRVFTAAP FDVSFGAKAF VDDFNNKFYA TQAGSNWTVG 2550
    INKFNCGWDE LARRFNPDWK FIDADGSRYD SSLTPLLFNA VLRIRQHFLR 2600
    ANGFERRMLS NFYTQLVWTP ISTITGQIVK KNKGGPSGQP STVVDNTMML 2650
    MIAVEYAKLQ YGVTDLKYVC NGDDLILNAP QGVCETIRAN FSHSFKELGL 2700
    TYEFEQEVDS IDQVEYMSHK WIDCGGVLIP KLKPERIVSV LQWNKSLDLA 2750
    SQANKINAAW IESFGYGDLS KFIREYANWW GERNGQVGFL CSEEKVASLY 2800
    LTNDVTIHTE EHDEFVFHSG ADQSGVVKDQ TGDKAEGSGT KTEDPPNQTT 2850
    DPVNNPSNGG NKDAPQNLNA TVVTKSYTYI PPIMKSLVTI DTAKKMADYT 2900
    PPDALISTQA CTLEQFGRWA NAAANGLGLS MQAFQTDVVP YWIYWCIVNS 2950
    ASDEHKKLSS WTKVNMTIDD ATGQINLNEG EAQTIYEMSP MFDEAKPTLR 3000
    AVMRHFGALA YRWVKFSIAK RKPIIPHNAI KAGLMDVTYF PCCIDFVTVD 3050
    QLSPQEQNVR NQVINARVSD TPRALFKHAQ RAGAGEEDTN LRRDDDANYG 3100
    RTRVGGAMFG TR 3112
    Length:3,112
    Mass (Da):352,841
    Last modified:July 13, 2010 - v3
    Checksum:i5B066BBD6C5D5151
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti2819 – 28191S → E AA sequence 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FJ263671 Genomic RNA. Translation: ABO41208.2.
    RefSeqiYP_002956073.1. NC_012799.1.

    Genome annotation databases

    GeneIDi7984336.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FJ263671 Genomic RNA. Translation: ABO41208.2 .
    RefSeqi YP_002956073.1. NC_012799.1.

    3D structure databases

    ProteinModelPortali A4KZ49.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 7984336.

    Family and domain databases

    Gene3Di 3.40.50.300. 2 hits.
    InterProi IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    IPR025910. P1_Ser_Pept_dom.
    IPR001456. Peptidase_C6.
    IPR001592. Poty_coat.
    IPR001730. Potyv_NIa-pro_dom.
    IPR013648. PP_Potyviridae.
    IPR001205. RNA-dir_pol_C.
    IPR007094. RNA-dir_pol_PSvirus.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00270. DEAD. 1 hit.
    PF00271. Helicase_C. 1 hit.
    PF00863. Peptidase_C4. 1 hit.
    PF00851. Peptidase_C6. 1 hit.
    PF13611. Peptidase_S76. 1 hit.
    PF00767. Poty_coat. 1 hit.
    PF08440. Poty_PP. 1 hit.
    PF00680. RdRP_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00966. NIAPOTYPTASE.
    SMARTi SM00487. DEXDc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    SSF52540. SSF52540. 1 hit.
    PROSITEi PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    PS51436. POTYVIRUS_NIA_PRO. 1 hit.
    PS50507. RDRP_SSRNA_POS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The complete genome sequence of Triticum mosaic virus, a new wheat-infecting virus of the High Plains."
      Fellers J.P., Seifers D., Ryba-White M., Martin T.J.
      Arch. Virol. 154:1511-1515(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. "Triticum mosaic virus: A new virus isolated from wheat in Kansas."
      Seifers D.L., Martin T.J., Harvey T.L., Fellers J.P., Stack J.P., Ryba-White M., Haber S., Krokhin O.V., Spicer V., Lovat N., Yamchuk A., Standing K.G.
      Plant Dis. 92:808-817(2008)
      Cited for: PROTEIN SEQUENCE OF 2819-2918; 2953-2968; 2988-3000; 3005-3012 AND 3016-3112.

    Entry informationi

    Entry nameiPOLG_TRMVU
    AccessioniPrimary (citable) accession number: A4KZ49
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 29, 2007
    Last sequence update: July 13, 2010
    Last modified: October 1, 2014
    This is version 45 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3