A4KSK1 (A4KSK1_FRATU) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
L-threonine 3-dehydrogenase HAMAP MF_00627
EC=1.1.1.103 HAMAP MF_00627

Gene names

Name:	tdh HAMAP MF_00627
ORF Names:	FTHG_01419 EMBL EBA52994.1

Organism

Francisella tularensis subsp. holarctica 257 EMBL EBA52994.1

Taxonomic identifier

412422 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Thiotrichales › Francisellaceae › Francisella

Protein attributes

Sequence length	351 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	L-threonine + NAD+ = L-2-amino-3-oxobutanoate + NADH. HAMAP MF_00627 SAAS SAAS002328
Cofactor	Binds 2 zinc ions per subunit By similarity. HAMAP MF_00627 SAAS SAAS002328
Pathway	Amino-acid degradation; L-threonine degradation via oxydo-reductase pathway; glycine from L-threonine: step 1/2. HAMAP MF_00627 SAAS SAAS002328
Subunit structure	Homotetramer By similarity. HAMAP MF_00627
Subcellular location	Cytoplasm By similarity HAMAP MF_00627 SAAS SAAS002328.
Sequence similarities	Belongs to the zinc-containing alcohol dehydrogenase family. HAMAP MF_00627 RuleBase RU000468

Ontologies

Keywords
Cellular component	Cytoplasm HAMAP MF_00627 SAAS SAAS002328
Ligand	Metal-binding HAMAP MF_00627 SAAS SAAS004627 NAD HAMAP MF_00627 SAAS SAAS002328 Zinc HAMAP MF_00627 SAAS SAAS004627
Molecular function	Oxidoreductase HAMAP MF_00627 SAAS SAAS004627
Gene Ontology (GO)
Biological process	threonine catabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-threonine 3-dehydrogenase activity Inferred from electronic annotation. Source: HAMAP nucleotide binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Metal binding	39	1	Zinc 1; catalytic By similarity HAMAP MF_00627
	Metal binding	64	1	Zinc 1; catalytic By similarity HAMAP MF_00627
	Metal binding	94	1	Zinc 2 By similarity HAMAP MF_00627
	Metal binding	97	1	Zinc 2 By similarity HAMAP MF_00627
	Metal binding	100	1	Zinc 2 By similarity HAMAP MF_00627
	Metal binding	108	1	Zinc 2 By similarity HAMAP MF_00627
	Metal binding	149	1	Zinc 1; catalytic By similarity HAMAP MF_00627

Sequences

	Sequence		Length	Mass (Da)
	A4KSK1 [UniParc]. Last modified May 1, 2007. Version 1. Checksum: 1E07E9CCC5A58A79	FASTA	351	38,400
	10 20 30 40 50 60 MKALAKLKKQ PGIWMINDAP IPEYGYNDVL IKIKKTAICG TDLHIYNWDK WSQNTIPVPM 70 80 90 100 110 120 ITGHEFAGEV VAKGDGVTSV DIGDRVSGEG HLVCGQCRNC RAGKRHLCRK TIGIGVNVQG 130 140 150 160 170 180 AFAEYLVMPA VNVFKIPDSI SDDIASTFDP MGNAIHTALS FNLTGEDVLI TGAGPIGLMA 190 200 210 220 230 240 VKIARFCGAR RIVITDINEY RLQMARDFGA TVALNVAPFK NQDELVKQMR KVMSDIGMTE 250 260 270 280 290 300 GFDVGLEMSG INSAISMMLD VMNHGGKLSL LGISAGDISV DWGAILFKGL TLKGIYGREM 310 320 330 340 350 FETWYLMTSM LQAGMDMNPI ITHRLHIDEF QKGFEIMKSG QCGKVILDWS S

References

[1]

"The Genome Sequence of Francisella tularensis subsp. holarctica Strain 257." The Broad Institute Genome Sequencing Platform Birren B., Lander E., Galagan J., Nusbaum C., Devon K., Borowsky M.L., Jaffe D., Butler J., Alvarez P., Gnerre S., Grabherr M., Mauceli E., Brockman W., Young S., LaButti K., Sykes S., DeCaprio D., Crawford M. , Koehrsen M., Engels R., Montgomery P., Pearson M., Howarth C., Larson L., White J., Zeng Q., Kodira C., Yandava C., Alvarado L., O'Leary S., Forsman M., Garcia E. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: 257 EMBL EBA52994.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DS229056 Genomic DNA. Translation: EBA52994.1.
3D structure databases
ProteinModelPortal	A4KSK1.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
PATRIC	27637789. VBIFraTul12999_1883.
Family and domain databases
HAMAP	MF_00627. Thr_dehydrog. [Tree]
InterPro	IPR013149. ADH_C. IPR013154. ADH_GroES-like. IPR002085. ADH_SF_Zn-type. IPR002328. ADH_Zn_CS. IPR011032. GroES-like. IPR004627. L-Threonine_3-DHase. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11695. ADH_Sf_Zn. 1 hit.
Pfam	PF08240. ADH_N. 1 hit. PF00107. ADH_zinc_N. 1 hit. [Graphical view]
SUPFAM	SSF50129. GroES_like. 1 hit.
TIGRFAMs	TIGR00692. Tdh. 1 hit.
PROSITE	PS00059. ADH_ZINC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

A4KSK1_FRATU

Accession

Primary (citable) accession number: A4KSK1

Entry history

Integrated into UniProtKB/TrEMBL:	May 1, 2007
Last sequence update:	May 1, 2007
Last modified:	December 14, 2011
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)