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UniProtKB - A4K150 (PA_I54A2)

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Protein

Polymerase acidic protein

Gene

PA

Organism
Influenza A virus (strain A/Malaysia:Malaya/302/1954 H1N1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Plays an essential role in viral RNA transcription and replication by forming the heterotrimeric polymerase complex together with PB1 and PB2 subunits. The complex transcribes viral mRNAs by using a unique mechanism called cap-snatching. It consists in the hijacking and cleavage of host capped pre-mRNAs. These short capped RNAs are then used as primers for viral mRNAs. The PB2 subunit is responsible for the binding of the 5' cap of cellular pre-mRNAs which are subsequently cleaved after 10-13 nucleotides by the PA subunit that carries the endonuclease activity.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 2 manganese ions per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi41Manganese 1; via tele nitrogenUniRule annotation1
Metal bindingi80Manganese 2UniRule annotation1
Metal bindingi108Manganese 1UniRule annotation1
Metal bindingi108Manganese 2UniRule annotation1
Metal bindingi119Manganese 1UniRule annotation1
Metal bindingi120Manganese 1; via carbonyl oxygenUniRule annotation1

GO - Molecular functioni

Complete GO annotation...

GO - Biological processi

Complete GO annotation...

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease
Biological processCap snatching, Eukaryotic host gene expression shutoff by virus, Eukaryotic host transcription shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction, Inhibition of host RNA polymerase II by virus
LigandManganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Polymerase acidic proteinUniRule annotation (EC:3.1.-.-UniRule annotation)
Alternative name(s):
RNA-directed RNA polymerase subunit P2UniRule annotation
Gene namesi
Name:PAUniRule annotation
OrganismiInfluenza A virus (strain A/Malaysia:Malaya/302/1954 H1N1)
Taxonomic identifieri425566 [NCBI]
Taxonomic lineageiViruses › ssRNA viruses › ssRNA negative-strand viruses › Orthomyxoviridae › Influenzavirus A ›
Virus hostiAves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]
Proteomesi
  • UP000008219 Componenti: Genome

Subcellular locationi

  • Host cytoplasm UniRule annotation
  • Host nucleus UniRule annotation

    • Note: PB1 and PA are transported in the host nucleus as a complex.UniRule annotationBy similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003730021 – 716Polymerase acidic proteinAdd BLAST716

Post-translational modificationi

Phosphorylated on serines and threonines by host kinases, including human casein kinase II.UniRule annotationBy similarity

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Influenza RNA polymerase is composed of three subunits: PB1, PB2 and PA. Interacts (via C-terminus) with PB1 (via N-terminus).UniRule annotationBy similarity

Structurei

3D structure databases

SMRiA4K150.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi124 – 139Nuclear localization signal 1 (NLS1)UniRule annotationBy similarityAdd BLAST16
Motifi184 – 247Nuclear localization signal 2 (NLS2)UniRule annotationBy similarityAdd BLAST64

Sequence similaritiesi

Belongs to the influenza viruses PA family.UniRule annotation

Phylogenomic databases

OrthoDBiVOG0900002P.

Family and domain databases

HAMAPiMF_04063. INFV_PA. 1 hit.
InterProiView protein in InterPro
IPR001009. RNA-dir_pol_influenzavirus.
PfamiView protein in Pfam
PF00603. Flu_PA. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by ribosomal frameshifting. AlignAdd to basket

Isoform PA (identifier: A4K150-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEDFVRQCFN PMIVELAEKA MKEYGENLKI ETNKFAAICT HLEVCFMYSD 
        60         70         80         90        100
FHFINEQGES IIVELDDPNA LLKHRFEIIE GRDRTMAWTV VNSICNTTGA 
       110        120        130        140        150
EKPKFLPDLY DYKENRFIEI GVTRREVHIY YLEKANKIKS EKTHIHIFSF 
       160        170        180        190        200
TGEEMATKAD YTLDEESRAR IKTRLFTIRQ EMASRGLWDS FRQSERGEET 
       210        220        230        240        250
IEERFEITGT MRRLADQSLP PNFSCLENFR AYVDGFEPNG YIEGKLSQMS 
       260        270        280        290        300
KEVNARIEPF LKTTPRPIRL PDGPPCSQRS KFLLMDALKL SIEDPSHEGE 
       310        320        330        340        350
GIPLYDAIKC MRTFFGWKEP YVVKPHEKGI NPNYLLSWKQ VLAELQDIEN 
       360        370        380        390        400
EEKIPRTKNM KKTSQLKWAL GENMAPEKVD FDDCKDISDL KQYDSDEPEL 
       410        420        430        440        450
RSLSSWIQNE FNKACELTDS IWIELDEIGE DVAPIEHIAS MRRNYFTAEV 
       460        470        480        490        500
SHCRATEYIM KGVYINTALL NASCAAMDDF QLIPMISKCR TKEGRRKTNL 
       510        520        530        540        550
YGFIIKGRSH LRNDTDVVNF VSMEFSLTDP RLEPHKWEKY CVLEIGDMLL 
       560        570        580        590        600
RSAIGQVSRP MFLYVRTNGT SKIKMKWGME MRRCLLQSLQ QIESMIEAES 
       610        620        630        640        650
SVKEKDMTKE FFENKSETWP IGESPKGVEE GSIGKVCRTL LAKSVFNSLY 
       660        670        680        690        700
ASPQLEGFSA ESRKLLLIVQ ALRDNLEPGT FDLGGLYEAI EECLINDPWV 
       710 
LLNASWFNSF LTHALR
Length:716
Mass (Da):82,847
Last modified:May 1, 2007 - v1
Checksum:i1DD9DD8F1CBBBCC3
GO
Isoform PA-X (identifier: P0DJS7-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry P0DJS7.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:252
Mass (Da):29,454
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CY021058 Viral cRNA. Translation: ABO52287.1.

Keywords - Coding sequence diversityi

Ribosomal frameshifting

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiPA_I54A2
AccessioniPrimary (citable) accession number: A4K150
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: May 1, 2007
Last modified: June 7, 2017
This is version 34 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families