Reviewed, UniProtKB/Swiss-Prot A4JR39 (GLGB_BURVG)

Names and origin

Protein names

Recommended name:
    1,4-alpha-glucan-branching enzyme
    EC=2.4.1.18
Alternative name(s):
    Glycogen-branching enzyme
      Short name=BE
    1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase

Gene names

Name:	glgB
Ordered Locus Names:	Bcep1808_5812

Organism

Burkholderia vietnamiensis (strain G4 / LMG 22486) (Burkholderia cepacia (strain R1808)) [Complete proteome] [HAMAP]

Taxonomic identifier

269482 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	733 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP MF_00685
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685
Subunit structure	Monomer By similarity. HAMAP MF_00685
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
Biological process	Glycogen biosynthesis
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: HAMAP cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 733	733	1,4-alpha-glucan-branching enzyme HAMAP MF_00685		PRO_1000044973
Sites
	Active site	307	1	By similarity
	Active site	342	1	By similarity
	Active site	347	1	By similarity
	Active site	410	1	By similarity
	Active site	412	1	By similarity
	Active site	467	1	By similarity
	Active site	534	1	By similarity
	Active site	535	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4JR39-1 [UniParc]. Last modified May 1, 2007. Version 1. Checksum: E401B84EAF687D26	FASTA	733	80,466
	10 20 30 40 50 60 MTDTLFDRRD IDALLGGRHP DPFACLGPHG EAGRTVVRAL LPGALRVRAV TPGGDELGAL 70 80 90 100 110 120 ACVDEAGCFA GTITHDGRYR LAIDWPDAQQ VTDDAYAFGT LLDDAALARF AAGDPAAVLD 130 140 150 160 170 180 CLGATPIRVD GVDGVRFAVW APNAQRVSVV GNFNSWDGRR HPMRLRRPWG VWELFVPGIG 190 200 210 220 230 240 PGEHYKYELC AADGSLLPHK ADPCARATEA PPRTASVVAD TAALDGFGWH DAGWIDARPD 250 260 270 280 290 300 AGRRFRVPWS IYEVHAESWQ RVPEEMDRSA TWDELAERLI PYVKGMGFTH VEFMPISEYP 310 320 330 340 350 360 FGGSWGYQPL AQFAPSARFG PVDGFARFVD RAHAAGIGVI VDWVPAHFPN DAHGLAQFDG 370 380 390 400 410 420 SALYEHADPR EGMHPDWNTC VFNLGRNEVS AFLIASALAW ARRYHVDGIR VDAVASMLYR 430 440 450 460 470 480 DYSRKEGEWV PNVYGGRENL ESVAFLRTLN DALHGAAAPA GVVTIAEEST AWPGVTAPTG 490 500 510 520 530 540 DGGLGFDFKW NMGWMHDTLA YLHEDPIHRR YHHDRMTFGL VYAFSERFVL PLSHDEVVHG 550 560 570 580 590 600 KGSLAAKMPD DAWQRLATLR AYFGFMWAHP GKKLLFMGSE FAQWSEFAHD GTPHWDLLDA 610 620 630 640 650 660 PAHRGVQRLV RDLNRAYASE PALHALDCDA AGFTWLIGDD RDNSVLAFAR RDDSGRVVVA 670 680 690 700 710 720 ICNFTPVPRS GYRVGLPAPG QWRELMNTDA AVYGGSNAGN DGAVWAQDVP AHGQPWSATL 730 RLPPLATLWL APA

References

[1]

"Complete sequence of chromosome 3 of Burkholderia vietnamiensis G4." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Tiedje J., Richardson P. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000616 Genomic DNA. Translation: ABO58742.1.
RefSeq	YP_001114997.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A4JR39.
Genome annotation databases
GeneID	4948716.
GenomeReviews	Gene locus Bcep1808_5812 in contig CP000616_GR.
KEGG	bvi:Bcep1808_5812.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0296.
HOGENOM	HBG287139.
OMA	PKTASIV.
Family and domain databases
HAMAP	MF_00685. GlgB. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz_core. IPR006048. A-amylase_b_C. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR017853. Glyco_hydro_catalytic_core. IPR013781. Glyco_hydro_sg_catalytic. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
TIGRFAMs	TIGR01515. branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLGB_BURVG

Accession

Primary (citable) accession number: A4JR39

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 1, 2007
Last modified:	February 9, 2010
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)