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Protein

1,4-alpha-glucan branching enzyme GlgB

Gene

glgB

Organism
Burkholderia vietnamiensis (strain G4 / LMG 22486) (Burkholderia cepacia (strain R1808))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.UniRule annotation

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei412 – 4121NucleophileUniRule annotation
Active sitei467 – 4671Proton donorUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

BioCyciBVIE269482:GJNA-5900-MONOMER.
UniPathwayiUPA00164.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgBUniRule annotation (EC:2.4.1.18UniRule annotation)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferaseUniRule annotation
Alpha-(1->4)-glucan branching enzymeUniRule annotation
Glycogen branching enzymeUniRule annotation
Short name:
BEUniRule annotation
Gene namesi
Name:glgBUniRule annotation
Ordered Locus Names:Bcep1808_5812
OrganismiBurkholderia vietnamiensis (strain G4 / LMG 22486) (Burkholderia cepacia (strain R1808))
Taxonomic identifieri269482 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex
ProteomesiUP000002287 Componenti: Chromosome 3

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7337331,4-alpha-glucan branching enzyme GlgBPRO_1000044973Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi269482.Bcep1808_5812.

Structurei

3D structure databases

ProteinModelPortaliA4JR39.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiYWFEQFH.
OrthoDBiEOG6JX7GT.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

Sequencei

Sequence statusi: Complete.

A4JR39-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDTLFDRRD IDALLGGRHP DPFACLGPHG EAGRTVVRAL LPGALRVRAV
60 70 80 90 100
TPGGDELGAL ACVDEAGCFA GTITHDGRYR LAIDWPDAQQ VTDDAYAFGT
110 120 130 140 150
LLDDAALARF AAGDPAAVLD CLGATPIRVD GVDGVRFAVW APNAQRVSVV
160 170 180 190 200
GNFNSWDGRR HPMRLRRPWG VWELFVPGIG PGEHYKYELC AADGSLLPHK
210 220 230 240 250
ADPCARATEA PPRTASVVAD TAALDGFGWH DAGWIDARPD AGRRFRVPWS
260 270 280 290 300
IYEVHAESWQ RVPEEMDRSA TWDELAERLI PYVKGMGFTH VEFMPISEYP
310 320 330 340 350
FGGSWGYQPL AQFAPSARFG PVDGFARFVD RAHAAGIGVI VDWVPAHFPN
360 370 380 390 400
DAHGLAQFDG SALYEHADPR EGMHPDWNTC VFNLGRNEVS AFLIASALAW
410 420 430 440 450
ARRYHVDGIR VDAVASMLYR DYSRKEGEWV PNVYGGRENL ESVAFLRTLN
460 470 480 490 500
DALHGAAAPA GVVTIAEEST AWPGVTAPTG DGGLGFDFKW NMGWMHDTLA
510 520 530 540 550
YLHEDPIHRR YHHDRMTFGL VYAFSERFVL PLSHDEVVHG KGSLAAKMPD
560 570 580 590 600
DAWQRLATLR AYFGFMWAHP GKKLLFMGSE FAQWSEFAHD GTPHWDLLDA
610 620 630 640 650
PAHRGVQRLV RDLNRAYASE PALHALDCDA AGFTWLIGDD RDNSVLAFAR
660 670 680 690 700
RDDSGRVVVA ICNFTPVPRS GYRVGLPAPG QWRELMNTDA AVYGGSNAGN
710 720 730
DGAVWAQDVP AHGQPWSATL RLPPLATLWL APA
Length:733
Mass (Da):80,466
Last modified:May 1, 2007 - v1
Checksum:iE401B84EAF687D26
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000616 Genomic DNA. Translation: ABO58742.1.
RefSeqiWP_011879949.1. NC_009254.1.
YP_001114997.1. NC_009254.1.

Genome annotation databases

EnsemblBacteriaiABO58742; ABO58742; Bcep1808_5812.
KEGGibvi:Bcep1808_5812.
PATRICi19312364. VBIBurVie89221_1202.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000616 Genomic DNA. Translation: ABO58742.1.
RefSeqiWP_011879949.1. NC_009254.1.
YP_001114997.1. NC_009254.1.

3D structure databases

ProteinModelPortaliA4JR39.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi269482.Bcep1808_5812.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABO58742; ABO58742; Bcep1808_5812.
KEGGibvi:Bcep1808_5812.
PATRICi19312364. VBIBurVie89221_1202.

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiYWFEQFH.
OrthoDBiEOG6JX7GT.

Enzyme and pathway databases

UniPathwayiUPA00164.
BioCyciBVIE269482:GJNA-5900-MONOMER.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of chromosome 3 of Burkholderia vietnamiensis G4."
    US DOE Joint Genome Institute
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M.
    , Hauser L., Kyrpides N., Tiedje J., Richardson P.
    Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: G4 / LMG 22486.

Entry informationi

Entry nameiGLGB_BURVG
AccessioniPrimary (citable) accession number: A4JR39
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 1, 2007
Last modified: April 29, 2015
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.