Reviewed, UniProtKB/Swiss-Prot A4JPG0 (KATG2_BURVG)

Names and origin

Protein names

Recommended name:
    Catalase-peroxidase 2
      Short name=CP 2
    EC=1.11.1.6
    EC=1.11.1.7
Alternative name(s):
    Peroxidase/catalase 2

Gene names

Name:	katG2
Ordered Locus Names:	Bcep1808_5214

Organism

Burkholderia vietnamiensis (strain G4 / LMG 22486) (Burkholderia cepacia (strain R1808)) [Complete proteome] [HAMAP]

Taxonomic identifier

269482 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	741 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity.
Catalytic activity	2 H2O2 = O2 + 2 H2O. HAMAP MF_01961 Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity.
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity.
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Domain	Signal
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: HAMAP heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 28	28	Potential
	Chain	29 – 741	713	Catalase-peroxidase 2 HAMAP MF_01961		PRO_5000232530
Sites
	Active site	108	1	Proton acceptor By similarity
	Metal binding	269	1	Iron (heme axial ligand) By similarity
	Site	104	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	107 ↔ 228		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-254) By similarity
	Cross-link	228 ↔ 254		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-107) By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4JPG0-1 [UniParc]. Last modified May 1, 2007. Version 1. Checksum: D9D4B4A38E9E7964	FASTA	741	80,509
	10 20 30 40 50 60 MQRNRIAKSV LAALAVIAMS AGSISARADG APRANGFWWP ETLDLSPLRQ HDVESNPYGK 70 80 90 100 110 120 DFDYAQAFQK LDIEAVKKDI RTTLTTSQDW WPADYGNYGP FFIRMAWHGA GTYRTYDGRG 130 140 150 160 170 180 GAGGAQQRFE PLNSWPDNAN LDKARRLLWP IKKKYGQNIS WGDLMVLTGN VALESMGFQT 190 200 210 220 230 240 FGFGGGREDD WQSDLVYWGA GPKFMSNNRD KNGKLEKPLA ATQMGLIYVN PEGPNGNPDP 250 260 270 280 290 300 VAAAQDIREA FGRMAMNDEE TLALIAGGHT FGKAHGAASP DKCVGVAPAG AGVEAQGLGW 310 320 330 340 350 360 ANKCGTGKGV DTITSGLEGA WSVDPVHFTM QYLDNLLGHD WVLTKSPAGA HQWMPKDAQS 370 380 390 400 410 420 IVPDAHDPSK LHPLMMFTTD IALKVDPAYS KIAKRFEEHP DEFKLAFAKA WFKLTHRDLG 430 440 450 460 470 480 PKARYLGKDV PKVDLIWQDP LPVAGYQTIG AADIADLKSR ILASGLPKSE LIKTAWASAA 490 500 510 520 530 540 SFRGTDYRGG ANGARIRLAP ENGWAVNDPA SVAKVLKSLE AIQSGFNKGR TDGKQVSLAD 550 560 570 580 590 600 LIVLGGSAAV EDAARKAGYD ITVPFAPGRV DATQAQTDVA SFAVLEPTAD GFRNYYQKGN 610 620 630 640 650 660 ERSPAELLVD RASKLDLTVP EMTVLVGGLR ALDANTGHSK LGVLTNHPGT LSNEFFVNLL 670 680 690 700 710 720 DMSTEWKKSP SADGTYEGRD RKTGALRWTA SPVDLVFGSS SELRAVAEVY ASDDAHEKFV 730 740 RDFVAAWTKV MNLDRFDLKR I

References

[1]

"Complete sequence of chromosome 2 of Burkholderia vietnamiensis G4." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Tiedje J., Richardson P. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000615 Genomic DNA. Translation: ABO58163.1.
RefSeq	YP_001117628.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4951432.
GenomeReviews	Gene locus Bcep1808_5214 in contig CP000615_GR.
KEGG	bvi:Bcep1808_5214.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A4JPG0. GTDMRGG.
Family and domain databases
HAMAP	MF_01961. [Tree]
InterPro	IPR000763. Catalase_proxase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
TIGRFAMs	TIGR00198. cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG2_BURVG

Accession

Primary (citable) accession number: A4JPG0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	May 1, 2007
Last modified:	June 16, 2009
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)