Reviewed, UniProtKB/Swiss-Prot A4JKE6 (MDH1_BURVG)

Names and origin

Protein names

Recommended name:
    Malate dehydrogenase 1
    EC=1.1.1.37

Gene names

Name:	mdh1
Ordered Locus Names:	Bcep1808_3766

Organism

Burkholderia vietnamiensis (strain G4 / LMG 22486) (Burkholderia cepacia (strain R1808)) [Complete proteome] [HAMAP]

Taxonomic identifier

269482 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	328 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP MF_01517
Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP MF_01517
Subunit structure	Homodimer By similarity. HAMAP MF_01517
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 2 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Molecular function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 328	328	Malate dehydrogenase 1 HAMAP MF_01517		PRO_0000315545
Regions
	Nucleotide binding	12 – 18	7	NAD By similarity
	Nucleotide binding	130 – 132	3	NAD By similarity
Sites
	Active site	188	1	Proton acceptor By similarity
	Binding site	93	1	Substrate By similarity
	Binding site	99	1	Substrate By similarity
	Binding site	106	1	NAD By similarity
	Binding site	113	1	NAD By similarity
	Binding site	132	1	Substrate By similarity
	Binding site	163	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4JKE6-1 [UniParc]. Last modified May 1, 2007. Version 1. Checksum: A861F980DA9E8285	FASTA	328	35,654
	10 20 30 40 50 60 MSKPARRVAV TGAAGQIAYS LLFRIARGDL LGDDQPVILQ LLELPHALDA LRGVVMELED 70 80 90 100 110 120 CAFPLLQSVE ISDDPRVAFR DADYAMLVGS RPRGKGMERR DLLAANAAIF RSQGEALNEV 130 140 150 160 170 180 ANRQVKVLVV GNPANTNAWV ARHYAPDLPA DAITAMIRLD HNRAVSKLAA RCGVTVDAVS 190 200 210 220 230 240 RMAVWGNHSP TMFPDYRHAL IDQQPAPMRV GDERWYLDTF IPEVARRGTA IIEARGASSA 250 260 270 280 290 300 ASAANAAIDQ MRDWIRGSGG RWVSMSIVSG GEYGIPRGLM FGMPTICSEG RYRVVPDLEI 310 320 DALARARIDA SVAELVDEMQ AVRAILAL

References

[1]

"Complete sequence of chromosome 2 of Burkholderia vietnamiensis G4." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Tiedje J., Richardson P. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000615 Genomic DNA. Translation: ABO56749.1.
RefSeq	YP_001116214.1.
3D structure databases
SMR	A4JKE6. Positions 3-322.
ModBase	Search...
Protein-protein interaction databases
STRING	A4JKE6.
Genome annotation databases
GeneID	4949983.
GenomeReviews	Gene locus Bcep1808_3766 in contig CP000615_GR.
KEGG	bvi:Bcep1808_3766.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0039.
HOGENOM	HBG289884.
OMA	FPDYRHA.
Family and domain databases
HAMAP	MF_01517. Malate_dehydrog_2. [Tree]
InterPro	IPR001557. L-lactate/malate_DH. IPR001236. Lactate/malate_DH. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR010945. Malate_DH_SF1. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR23382. MDH_SF1. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
TIGRFAMs	TIGR01759. MalateDH-SF1. 1 hit.
PROSITE	PS00068. MDH. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MDH1_BURVG

Accession

Primary (citable) accession number: A4JKE6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 1, 2007
Last modified:	February 9, 2010
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)