Reviewed, UniProtKB/Swiss-Prot A4JJS1 (METE_BURVG)

Names and origin

Protein names

Recommended name:
    5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
    EC=2.1.1.14
Alternative name(s):
    Methionine synthase, vitamin-B12 independent isozyme
    Cobalamin-independent methionine synthase

Gene names

Name:	metE
Ordered Locus Names:	Bcep1808_3537

Organism

Burkholderia vietnamiensis (strain G4 / LMG 22486) (Burkholderia cepacia (strain R1808)) [Complete proteome] [HAMAP]

Taxonomic identifier

269482 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	767 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 767	767	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_1000017233
Sites
	Metal binding	650	1	Zinc By similarity
	Metal binding	652	1	Zinc By similarity
	Metal binding	735	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4JJS1-1 [UniParc]. Last modified May 1, 2007. Version 1. Checksum: A4CF72720A653F27	FASTA	767	85,940
	10 20 30 40 50 60 MVKTHNLGFP RIGAQRELKF GLERYWNGDA TRDELTALGA ALRRRHWSDQ RDLDLAPLGD 70 80 90 100 110 120 FAFYDHVLDM SFTLGNLPKR VQDFHGDALD NYFRVARGRS AHSAHEHASC CGGVAAGEMT 130 140 150 160 170 180 KWFDTNYHYI VPEFHADTNF SLDPSRLLQQ LAEANAQRVN AKPVILGPVT YLWLGKAKDD 190 200 210 220 230 240 SDRLALLPKL LPVYGALLDT LTAQGVEWVQ IDEPILVTEL DADWRRALRT AYEALETRRI 250 260 270 280 290 300 KLLLATYFGQ LQDNLELAVS LPVDGLHIDA VNARDEVDAL ARALPPTRVL SVGAIDGRNI 310 320 330 340 350 360 WRTDLNAALD WLEPLARQLG ERLWLAPSCS LLHVPVDLAS EQKLDAEIRS WLAFALQKLA 370 380 390 400 410 420 ELKLLATALN QGRDTVADAL AENAAAIESR RRSPRVHNPA VKSAVARIDA RLGNRASAYP 430 440 450 460 470 480 QRAAAQSARL KLPLFPTTTI GSFPQTAEIR DARRRFKAGE LDEAGYRHAM HAEIERSVRE 490 500 510 520 530 540 QESLQLDVLV HGEAERNDMV EYFGEQLDGY AFSQFGWVQS YGSRCVKPPI LFGDISRPSA 550 560 570 580 590 600 MTVEWIAYAQ SLTSKPMKGM LTGPVTILNW SFVRDDQPRS VSCYQLALAI RDEVLDLERA 610 620 630 640 650 660 GVRVIQIDEA ALREGLPLRR AQWPAYLKWA IDAFRIAANG VRDDTQIHTH MCYSEFNDII 670 680 690 700 710 720 ASIAEMDADV ITIETSRSDM ELLDAFDGFR YPNEIGPGVY DIHSPNIPTQ EHIVGLMRKA 730 740 750 760 AERIPAQRLW VNPDCGLKTR RWAEVLPALT NMVAAAKTLR SELRNGL

References

[1]

"Complete sequence of chromosome 2 of Burkholderia vietnamiensis G4." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Tiedje J., Richardson P. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000615 Genomic DNA. Translation: ABO56524.1.
RefSeq	YP_001115989.1.
3D structure databases
SMR	A4JJS1. Positions 3-762.
ModBase	Search...
Protein-protein interaction databases
STRING	A4JJS1.
Genome annotation databases
GeneID	4949749.
GenomeReviews	Gene locus Bcep1808_3537 in contig CP000615_GR.
KEGG	bvi:Bcep1808_3537.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0620.
HOGENOM	HBG287495.
OMA	SFVRNDI.
Family and domain databases
HAMAP	MF_00172. Meth_synth. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR002629. Methionine_synth. IPR006276. MeTrfase_B12_ind. [Graphical view]
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMs	TIGR01371. met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_BURVG

Accession

Primary (citable) accession number: A4JJS1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 1, 2007
Last modified:	February 9, 2010
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)