Skip Header

Contribute Send feedback
Read comments (?) or add your own

A4JIB6 (BIOD_BURVG) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 31. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ATP-dependent dethiobiotin synthetase BioD
EC=6.3.3.3
Alternative name(s):
DTB synthetase
Short name=DTBS
Dethiobiotin synthase
Gene names
Name:bioD
Ordered Locus Names:Bcep1808_3028
OrganismBurkholderia vietnamiensis (strain G4 / LMG 22486) (Burkholderia cepacia (strain R1808)) [Complete proteome] [HAMAP]
Taxonomic identifier269482 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length239 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA) to form an ureido ring By similarity. HAMAP MF_00336

Catalytic activity

ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336

Cofactor

Magnesium By similarity. HAMAP MF_00336

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336

Subcellular location

Cytoplasm By similarity HAMAP MF_00336.

Sequence similarities

Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
   Biological processBiotin biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processbiotin biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

dethiobiotin synthase activity

Inferred from electronic annotation. Source: EC

magnesium ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 239239ATP-dependent dethiobiotin synthetase BioD HAMAP MF_00336
PRO_1000019554

Regions

Nucleotide binding118 – 1214ATP By similarity
Nucleotide binding178 – 1792ATP By similarity

Sites

Metal binding151Magnesium 1 By similarity
Metal binding191Magnesium 2 By similarity
Metal binding571Magnesium 2 By similarity
Metal binding1181Magnesium 2 By similarity
Binding site571ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A4JIB6 [UniParc].

Last modified May 1, 2007. Version 1.
Checksum: 62A66C035C56E2BB

FASTA23925,246
        10         20         30         40         50         60 
MSTALSVFVT GTDTEIGKTF VSAAMLHGFA RHGLRAAALK PVAAGAYERD GIWRNEDADQ 

        70         80         90        100        110        120 
LDAAANVALP PELRTPFLLK APAAPHIVAA HEGVTLDIDT IVASHREALT RANVVVVEGV 

       130        140        150        160        170        180 
GGFRVPLNDT QDTADLAVAL GLPVVLVVGI RLGCISHALL TADAIRQRGL TLAGWVANHV 

       190        200        210        220        230 
DPAMSYADEN VATIRDWLAR EHRAPLLGRV AHLRPAAPES AAAMLDIAAL VDTLRRAQH

« Hide

References

[1]"Complete sequence of chromosome 1 of Burkholderia vietnamiensis G4."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Tiedje J., Richardson P.
Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: G4 / LMG 22486.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000614 Genomic DNA. Translation: ABO56019.1.
RefSeqYP_001120854.1. NC_009256.1.

3D structure databases

ProteinModelPortalA4JIB6.
ModBaseSearch...

Protein-protein interaction databases

STRINGA4JIB6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4954774.
GenomeReviewsGene locus Bcep1808_3028 in contig CP000614_GR.
KEGGbvi:Bcep1808_3028.
PATRIC19324716. VBIBurVie89221_7300.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0132.
HOGENOMHBG650065.
OMAANCVDPN.
ProtClustDBPRK00090.

Enzyme and pathway databases

BioCycBVIE269482:BCEP1808_3028-MONOMER.

Family and domain databases

HAMAPMF_00336. BioD.
[Tree]
InterProIPR002586. CbiA_P_synth.
IPR004472. DTB_synth_BioD.
[Graphical view]
KOK01935.
PfamPF01656. CbiA. 1 hit.
[Graphical view]
PIRSFPIRSF006755. DTB_synth. 1 hit.
TIGRFAMsTIGR00347. BioD. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOD_BURVG
AccessionPrimary (citable) accession number: A4JIB6
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 1, 2007
Last modified: December 14, 2011
This is version 31 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents