A4JIA9 (ACEK_BURVG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Isocitrate dehydrogenase kinase/phosphatase
Short name=IDH kinase/phosphatase
Short name=IDHK/P
EC=2.7.11.5
EC=3.1.3.-

Gene names

Name:	aceK
Ordered Locus Names:	Bcep1808_3021

Organism

Burkholderia vietnamiensis (strain G4 / LMG 22486) (Burkholderia cepacia (strain R1808)) [Complete proteome] [HAMAP]

Taxonomic identifier

269482 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	606 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source of carbon. When bacteria are grown on glucose, IDH is fully active and unphosphorylated, but when grown on acetate or ethanol, the activity of IDH declines drastically concomitant with its phosphorylation By similarity. HAMAP MF_00747
Catalytic activity	ATP + [isocitrate dehydrogenase (NADP+)] = ADP + [isocitrate dehydrogenase (NADP+)] phosphate. HAMAP MF_00747
Subcellular location	Cytoplasm By similarity HAMAP MF_00747.
Sequence similarities	Belongs to the AceK family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Kinase Protein phosphatase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW [isocitrate dehydrogenase (NADP+)] kinase activity Inferred from electronic annotation. Source: EC phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 606	606	Isocitrate dehydrogenase kinase/phosphatase HAMAP MF_00747		PRO_1000046551
Regions
	Nucleotide binding	327 – 333	7	ATP By similarity
Sites
	Active site	383	1	By similarity
	Binding site	348	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4JIA9 [UniParc]. Last modified May 1, 2007. Version 1. Checksum: 552052AF27033871	FASTA	606	70,106
	10 20 30 40 50 60 MNHFPKLLSS QIGFDIAQTM LEYFDRHYRI FREAAVEAKT LYERGDWHGL QRLARERITS 70 80 90 100 110 120 YDERVKECVE LLEDEYDAEN IDDEVWQQIK LHYIGLLTSH RQPECAETFF NSVCCKILHR 130 140 150 160 170 180 SYFNNDFIFV RPAISTEYLE NDEPAAKPTY RAYYPGTDGL AATLERIVTN FQLEPPFEDL 190 200 210 220 230 240 TRDIGCVMQA IDDEFGQFDA APDFQIHVLS SLFFRNKSAY IIGRIINADR VLPFAMPIRH 250 260 270 280 290 300 VRAGVLAVDT VLLRRELLQV IFSFSHSYFL VDMGVPSAYV DFLCTIMPGK PKAEIYTSVG 310 320 330 340 350 360 LQKQGKNLFY RDLLHHLSHS SDRFIIAPGI KGLVMLVFTL PSFPYVFKII KDHFPPPKET 370 380 390 400 410 420 TRAQIMEKYQ LVKRHDRLGR MADTLEYSSV ALPLSRLDHA LVRELEKEVP SLLEHEDDNL 430 440 450 460 470 480 VIEHLYIERR MTPLNLYLQN GSDADIEHGV KEYGNAVKEL MKANIFPGDM LYKNFGVTRH 490 500 510 520 530 540 GRVVFYDYDE IEYLTDCNVR RVPAPRNEED ELSGEPWYTV GPHDIFPETY GPFLLGDPRV 550 560 570 580 590 600 RTVFMKHHAD FFDPALWQAS KDKLLQGELP DFYPYDTSLR FCVRYAARFD ATPDHDDGAG AAQRAA

References

[1]

"Complete sequence of chromosome 1 of Burkholderia vietnamiensis G4." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Tiedje J., Richardson P. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: G4 / LMG 22486.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000614 Genomic DNA. Translation: ABO56012.1.
RefSeq	YP_001120847.1. NC_009256.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A4JIA9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4954767.
GenomeReviews	Gene locus Bcep1808_3021 in contig CP000614_GR.
KEGG	bvi:Bcep1808_3021.
PATRIC	19324700. VBIBurVie89221_7292.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG4579.
HOGENOM	HBG298593.
OMA	RMTPLNI.
ProtClustDB	PRK02946.
Enzyme and pathway databases
BioCyc	BVIE269482:BCEP1808_3021-MONOMER.
Family and domain databases
HAMAP	MF_00747. AceK. [Tree]
InterPro	IPR010452. Isocitrate_DH_AceK. [Graphical view]
KO	K00906.
Pfam	PF06315. AceK. 1 hit. [Graphical view]
PIRSF	PIRSF000719. AceK. 1 hit.
ProDom	PD043552. Isocitrate_DH_AceK. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

ACEK_BURVG

Accession

Primary (citable) accession number: A4JIA9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 1, 2007
Last modified:	January 25, 2012
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program