Reviewed, UniProtKB/Swiss-Prot A4JHT1 (QUEF_BURVG)

Names and origin

Protein names

Recommended name:
    NADPH-dependent 7-cyano-7-deazaguanine reductase
    EC=1.7.1.13
Alternative name(s):
    7-cyano-7-carbaguanine reductase
    PreQ(0) reductase
    NADPH-dependent nitrile oxidoreductase

Gene names

Name:	queF
Ordered Locus Names:	Bcep1808_2843

Organism

Burkholderia vietnamiensis (strain G4 / LMG 22486) (Burkholderia cepacia (strain R1808)) [Complete proteome] [HAMAP]

Taxonomic identifier

269482 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	274 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) By similarity.
Catalytic activity	7-aminomethyl-7-carbaguanine + 2 NADP(+) = 7-cyano-7-carbaguanine + 2 NADPH.
Pathway	tRNA modification; queuosine-tRNA biosynthesis.
Subcellular location	CytoplasmProbable.
Sequence similarities	Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.

Ontologies

Keywords
Biological process	Queuosine biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic compound biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW queuosine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	GTP cyclohydrolase I activity Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor Inferred from electronic annotation. Source: HAMAP queuine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 274	274	NADPH-dependent 7-cyano-7-deazaguanine reductase		PRO_1000062335

Sequences

	Sequence		Length	Mass (Da)
	A4JHT1-1 [UniParc]. Last modified May 1, 2007. Version 1. Checksum: 0ED5A700F3AF5B80	FASTA	274	30,291
	10 20 30 40 50 60 MNPEHSPLGK ATVYAAQYDA SLLFPIPRAG AREQLGIAAA LPFFGTDIWN AYELSWLNAR 70 80 90 100 110 120 GKPQLAVATF YVPAESPNIV ESKSFKLYLG SFAQSKFDSV DAVRDVLKRD VSAACGASVS 130 140 150 160 170 180 VQLVSPHDFG KLQMEELDGL SLDRLDLDTD VYEPDPSLLS AAADEAPVEE TLVSDLLRSN 190 200 210 220 230 240 CPVTGQPDWG SVQIHYVGPQ IDHAGLLRYI ISFRNHTGFH EQCVERIFLD ILQACKPLKL 250 260 270 AVYARYTRRG GLDINPFRTN YNQSMPDNAR TARQ

References

[1]

"Complete sequence of chromosome 1 of Burkholderia vietnamiensis G4." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Tiedje J., Richardson P. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000614 Genomic DNA. Translation: ABO55834.1.
RefSeq	YP_001120669.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4954588.
GenomeReviews	Gene locus Bcep1808_2843 in contig CP000614_GR.
KEGG	bvi:Bcep1808_2843.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00817. [Tree]
InterPro	IPR016428. CN_OxRdtase_NADPH-dep_YqcD. IPR001474. GTP_CycOHase_I. [Graphical view]
Pfam	PF01227. GTP_cyclohydroI. 1 hit. [Graphical view]
PIRSF	PIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
TIGRFAMs	TIGR03138. QueF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

QUEF_BURVG

Accession

Primary (citable) accession number: A4JHT1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	May 1, 2007
Last modified:	November 25, 2008
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)