A4JH86 (MURB_BURVG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylenolpyruvoylglucosamine reductase
EC=1.1.1.158

Alternative name(s):
UDP-N-acetylmuramate dehydrogenase

Gene names

Name:	murB
Ordered Locus Names:	Bcep1808_2647

Organism

Burkholderia vietnamiensis (strain G4 / LMG 22486) (Burkholderia cepacia (strain R1808)) [Complete proteome] [HAMAP]

Taxonomic identifier

269482 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	349 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation By similarity. HAMAP MF_00037
Catalytic activity	UDP-N-acetylmuramate + NADP+ = UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH. HAMAP MF_00037
Cofactor	FAD By similarity. HAMAP MF_00037
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00037
Subcellular location	Cytoplasm By similarity HAMAP MF_00037.
Sequence similarities	Belongs to the MurB family. Contains 1 FAD-binding PCMH-type domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	UDP-N-acetylmuramate dehydrogenase activity Inferred from electronic annotation. Source: EC flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 349	349	UDP-N-acetylenolpyruvoylglucosamine reductase HAMAP MF_00037		PRO_1000002876
Regions
	Domain	25 – 197	173	FAD-binding PCMH-type
Sites
	Active site	173	1	By similarity
	Active site	249	1	Proton donor By similarity
	Active site	345	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4JH86 [UniParc]. Last modified May 1, 2007. Version 1. Checksum: 207F73D088E81AD9	FASTA	349	37,764
	10 20 30 40 50 60 MPMPPDDSPL SLLPDHPLAA HNTFGIAATA RYAARITHAA QFEALHRDPR VASLPQLVLG 70 80 90 100 110 120 GGSNVVFTRD FDGVVLLDEI GGRRVVREDD DAWYVEAGGG EPWHAFVAWT LEQGMPGLEN 130 140 150 160 170 180 LALIPGTVGA APIQNIGAYG LEMKAYFDSL IAVELATGRS ERFDAARCAF GYRDSFFKRD 190 200 210 220 230 240 GRGRFAIVAV TFRLPKRWTP RLGYADVTRE LDARGIAPDA ATPRDVFDAV VAIRRAKLPD 250 260 270 280 290 300 PRVLGNAGSF FKNPVIDAAQ FDALRARAPD VVSYPQPDGQ VKLAAGWLID QCGWKGRALG 310 320 330 340 AAAVHERQAL VLVNRGGATG VDVLALARAI QHDVRVRFGV ELEPEPVCL

References

[1]

"Complete sequence of chromosome 1 of Burkholderia vietnamiensis G4." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Tiedje J., Richardson P. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: G4 / LMG 22486.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000614 Genomic DNA. Translation: ABO55639.1.
RefSeq	YP_001120474.1. NC_009256.1.
3D structure databases
ProteinModelPortal	A4JH86.
ModBase	Search...
Protein-protein interaction databases
STRING	A4JH86.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4954391.
GenomeReviews	Gene locus Bcep1808_2647 in contig CP000614_GR.
KEGG	bvi:Bcep1808_2647.
PATRIC	19323920. VBIBurVie89221_6903.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0812.
HOGENOM	HBG686573.
OMA	IEQAGWK.
ProtClustDB	PRK00046.
Enzyme and pathway databases
BioCyc	BVIE269482:BCEP1808_2647-MONOMER.
Family and domain databases
HAMAP	MF_00037. MurB. [Tree]
InterPro	IPR016169. CO_DH_flavot_FAD-bd_sub2. IPR016166. FAD-bd_2. IPR016167. FAD-bd_2_sub1. IPR003170. MurB. IPR011601. MurB_C. IPR006094. Oxid_FAD_bind_N. [Graphical view]
Gene3D	G3DSA:3.30.465.10. CO_DH_flavoprot_FAD-bd_sub2. 1 hit. G3DSA:3.30.43.10. FAD-binding_2_sub1. 1 hit. G3DSA:3.90.78.10. MurB_C. 1 hit.
KO	K00075.
PANTHER	PTHR21071. MurB. 1 hit.
Pfam	PF01565. FAD_binding_4. 1 hit. PF02873. MurB_C. 1 hit. [Graphical view]
SUPFAM	SSF56176. FAD-binding_2. 1 hit. SSF56194. MurB_C. 1 hit.
TIGRFAMs	TIGR00179. MurB. 1 hit.
PROSITE	PS51387. FAD_PCMH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MURB_BURVG

Accession

Primary (citable) accession number: A4JH86

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 1, 2007
Last modified:	December 14, 2011
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program