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A4JG73

- A4JG73_BURVG

UniProt

A4JG73 - A4JG73_BURVG

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Protein
Acetyl-coenzyme A synthetase
Gene
acsA, Bcep1808_2277
Organism
Burkholderia vietnamiensis (strain G4 / LMG 22486) (Burkholderia cepacia (strain R1808))
Status
Unreviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA By similarity.UniRule annotation

Catalytic activityi

ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei317 – 3171Coenzyme A By similarityUniRule annotation
Binding sitei397 – 3971Substrate; via nitrogen amide By similarityUniRule annotation
Binding sitei512 – 5121Substrate By similarityUniRule annotation
Binding sitei528 – 5281Substrate By similarityUniRule annotation
Active sitei530 – 5301 By similarityUniRule annotation
Binding sitei536 – 5361Coenzyme A By similarityUniRule annotation
Binding sitei539 – 5391Substrate By similarityUniRule annotation
Metal bindingi550 – 5501Magnesium; via carbonyl oxygen By similarityUniRule annotation
Metal bindingi552 – 5521Magnesium; via carbonyl oxygen By similarityUniRule annotation
Metal bindingi555 – 5551Magnesium; via carbonyl oxygen By similarityUniRule annotation
Binding sitei600 – 6001Coenzyme A By similarityUniRule annotation

GO - Molecular functioni

  1. AMP binding Source: InterPro
  2. ATP binding Source: UniProtKB-KW
  3. acetate-CoA ligase activity Source: UniProtKB-HAMAP
  4. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. acetyl-CoA biosynthetic process from acetate Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotationImported

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciBVIE269482:GJNA-2339-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetyl-coenzyme A synthetaseUniRule annotation (EC:6.2.1.1UniRule annotation)
Short name:
AcCoA synthetaseUniRule annotation
Short name:
AcsUniRule annotation
Alternative name(s):
Acetate--CoA ligase
Acyl-activating enzyme
Gene namesi
Name:acsAUniRule annotation
Ordered Locus Names:Bcep1808_2277Imported
OrganismiBurkholderia vietnamiensis (strain G4 / LMG 22486) (Burkholderia cepacia (strain R1808))Imported
Taxonomic identifieri269482 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex
ProteomesiUP000002287: Chromosome 1

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei625 – 6251N6-acetyllysine By similarityUniRule annotation

Post-translational modificationi

Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme By similarity.UniRule annotation

Keywords - PTMi

AcetylationUniRule annotation

Interactioni

Protein-protein interaction databases

STRINGi269482.Bcep1808_2277.

Structurei

3D structure databases

ProteinModelPortaliA4JG73.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni421 – 4266Substrate binding By similarityUniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0365.
HOGENOMiHOG000229981.
KOiK01895.
OMAiAWIWYRD.
OrthoDBiEOG68WR2H.

Family and domain databases

HAMAPiMF_01123. Ac_CoA_synth.
InterProiIPR011904. Ac_CoA_lig.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4JG73-1 [UniParc]FASTAAdd to Basket

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MSAIESVLHE TRQFAPPAAL EKTATISGMP AYRALVAEAE QDYEGFWARL    50
ARENLSWHKP FTKVLDESNA PFCTWFDDGE LNASYNCLDR HVEAGNGQRV 100
AVIFEADDGT VTRVTYAELL ARVSRFANAL KKRGIGKGDR VVIYIPMSIE 150
GIVAMQACAR IGATHSVVFG GFSAKSLNER LVDVGAVALI TADEQARGGK 200
TLPLKSIADE AIAMGGCDAV KSVIVYRRTG GKIDWHAGRD LWMHELADAE 250
SDHCPPEWVG AEHPLFILYT SGSTGKPKGV QHSTGGYLLW AAQTMKWTFD 300
WKPDDVFWCT ADIGWVTGHT YITYGPLACG GTQVVFEGVP TYPDAGRFWK 350
MIADHKVSVF YTAPTAIRSL IKAADADDKV HPKSYDLSSL RIIGTVGEPI 400
NPDAWMWYHK HVGGERCPIV DTWWQTETGG HMITPLPGAT PTVPGSCTLP 450
LPGIMAAVVD ETGQDVPNGQ GGILVVKRPW PSMIRTIWGD PERFKKSYYP 500
EELGGGLYLA GDGTVRDKDT GYFTIMGRID DVLNVSGHRL GTMEIESALV 550
SHELVAEAAV VGRPDDTTGE AVVAFVVLKR SRPEGDEAAA LAKALRDWVG 600
KQIGPIAKPK DIRFGDNLPK TRSGKIMRRL LRSLAKGEAI TQDTSTLENP 650
AILEQLTEVR 660
Length:660
Mass (Da):72,019
Last modified:May 1, 2007 - v1
Checksum:i175CF239E0DB3C32
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000614 Genomic DNA. Translation: ABO55276.1.
RefSeqiYP_001120111.1. NC_009256.1.

Genome annotation databases

EnsemblBacteriaiABO55276; ABO55276; Bcep1808_2277.
GeneIDi4954012.
KEGGibvi:Bcep1808_2277.
PATRICi19323128. VBIBurVie89221_6515.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000614 Genomic DNA. Translation: ABO55276.1 .
RefSeqi YP_001120111.1. NC_009256.1.

3D structure databases

ProteinModelPortali A4JG73.
ModBasei Search...

Protein-protein interaction databases

STRINGi 269482.Bcep1808_2277.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABO55276 ; ABO55276 ; Bcep1808_2277 .
GeneIDi 4954012.
KEGGi bvi:Bcep1808_2277.
PATRICi 19323128. VBIBurVie89221_6515.

Phylogenomic databases

eggNOGi COG0365.
HOGENOMi HOG000229981.
KOi K01895.
OMAi AWIWYRD.
OrthoDBi EOG68WR2H.

Enzyme and pathway databases

BioCyci BVIE269482:GJNA-2339-MONOMER.

Family and domain databases

HAMAPi MF_01123. Ac_CoA_synth.
InterProi IPR011904. Ac_CoA_lig.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view ]
Pfami PF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITEi PS00455. AMP_BINDING. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of chromosome 1 of Burkholderia vietnamiensis G4."
    US DOE Joint Genome Institute
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M.
    , Hauser L., Kyrpides N., Tiedje J., Richardson P.
    Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: G4 / LMG 22486.

Entry informationi

Entry nameiA4JG73_BURVG
AccessioniPrimary (citable) accession number: A4JG73
Entry historyi
Integrated into UniProtKB/TrEMBL: May 1, 2007
Last sequence update: May 1, 2007
Last modified: June 11, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome

External Data

Dasty 3

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