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A4JG73

- A4JG73_BURVG

UniProt

A4JG73 - A4JG73_BURVG

Protein

Acetyl-coenzyme A synthetase

Gene

acsA

Organism
Burkholderia vietnamiensis (strain G4 / LMG 22486) (Burkholderia cepacia (strain R1808))
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 49 (01 Oct 2014)
      Sequence version 1 (01 May 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA.UniRule annotation

    Catalytic activityi

    ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei317 – 3171Coenzyme AUniRule annotation
    Binding sitei397 – 3971Substrate; via nitrogen amideUniRule annotation
    Binding sitei512 – 5121SubstrateUniRule annotation
    Binding sitei528 – 5281SubstrateUniRule annotation
    Active sitei530 – 5301UniRule annotation
    Binding sitei536 – 5361Coenzyme AUniRule annotation
    Binding sitei539 – 5391SubstrateUniRule annotation
    Metal bindingi550 – 5501Magnesium; via carbonyl oxygenUniRule annotation
    Metal bindingi552 – 5521Magnesium; via carbonyl oxygenUniRule annotation
    Metal bindingi555 – 5551Magnesium; via carbonyl oxygenUniRule annotation
    Binding sitei600 – 6001Coenzyme AUniRule annotation

    GO - Molecular functioni

    1. acetate-CoA ligase activity Source: UniProtKB-HAMAP
    2. AMP binding Source: InterPro
    3. ATP binding Source: UniProtKB-KW
    4. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. acetyl-CoA biosynthetic process from acetate Source: InterPro

    Keywords - Molecular functioni

    LigaseUniRule annotationImported

    Keywords - Ligandi

    ATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciBVIE269482:GJNA-2339-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Acetyl-coenzyme A synthetaseUniRule annotation (EC:6.2.1.1UniRule annotation)
    Short name:
    AcCoA synthetaseUniRule annotation
    Short name:
    AcsUniRule annotation
    Alternative name(s):
    Acetate--CoA ligaseUniRule annotation
    Acyl-activating enzymeUniRule annotation
    Gene namesi
    Name:acsAUniRule annotation
    Ordered Locus Names:Bcep1808_2277Imported
    OrganismiBurkholderia vietnamiensis (strain G4 / LMG 22486) (Burkholderia cepacia (strain R1808))Imported
    Taxonomic identifieri269482 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex
    ProteomesiUP000002287: Chromosome 1

    PTM / Processingi

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei625 – 6251N6-acetyllysineUniRule annotation

    Post-translational modificationi

    Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme.UniRule annotation

    Keywords - PTMi

    AcetylationUniRule annotation

    Interactioni

    Protein-protein interaction databases

    STRINGi269482.Bcep1808_2277.

    Structurei

    3D structure databases

    ProteinModelPortaliA4JG73.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni421 – 4266Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the ATP-dependent AMP-binding enzyme family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0365.
    HOGENOMiHOG000229981.
    KOiK01895.
    OMAiAWIWYRD.
    OrthoDBiEOG68WR2H.

    Family and domain databases

    HAMAPiMF_01123. Ac_CoA_synth.
    InterProiIPR011904. Ac_CoA_lig.
    IPR025110. AMP-bd_C.
    IPR020845. AMP-binding_CS.
    IPR000873. AMP-dep_Synth/Lig.
    [Graphical view]
    PfamiPF00501. AMP-binding. 1 hit.
    PF13193. AMP-binding_C. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR02188. Ac_CoA_lig_AcsA. 1 hit.
    PROSITEiPS00455. AMP_BINDING. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A4JG73-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSAIESVLHE TRQFAPPAAL EKTATISGMP AYRALVAEAE QDYEGFWARL    50
    ARENLSWHKP FTKVLDESNA PFCTWFDDGE LNASYNCLDR HVEAGNGQRV 100
    AVIFEADDGT VTRVTYAELL ARVSRFANAL KKRGIGKGDR VVIYIPMSIE 150
    GIVAMQACAR IGATHSVVFG GFSAKSLNER LVDVGAVALI TADEQARGGK 200
    TLPLKSIADE AIAMGGCDAV KSVIVYRRTG GKIDWHAGRD LWMHELADAE 250
    SDHCPPEWVG AEHPLFILYT SGSTGKPKGV QHSTGGYLLW AAQTMKWTFD 300
    WKPDDVFWCT ADIGWVTGHT YITYGPLACG GTQVVFEGVP TYPDAGRFWK 350
    MIADHKVSVF YTAPTAIRSL IKAADADDKV HPKSYDLSSL RIIGTVGEPI 400
    NPDAWMWYHK HVGGERCPIV DTWWQTETGG HMITPLPGAT PTVPGSCTLP 450
    LPGIMAAVVD ETGQDVPNGQ GGILVVKRPW PSMIRTIWGD PERFKKSYYP 500
    EELGGGLYLA GDGTVRDKDT GYFTIMGRID DVLNVSGHRL GTMEIESALV 550
    SHELVAEAAV VGRPDDTTGE AVVAFVVLKR SRPEGDEAAA LAKALRDWVG 600
    KQIGPIAKPK DIRFGDNLPK TRSGKIMRRL LRSLAKGEAI TQDTSTLENP 650
    AILEQLTEVR 660
    Length:660
    Mass (Da):72,019
    Last modified:May 1, 2007 - v1
    Checksum:i175CF239E0DB3C32
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000614 Genomic DNA. Translation: ABO55276.1.
    RefSeqiYP_001120111.1. NC_009256.1.

    Genome annotation databases

    EnsemblBacteriaiABO55276; ABO55276; Bcep1808_2277.
    GeneIDi4954012.
    KEGGibvi:Bcep1808_2277.
    PATRICi19323128. VBIBurVie89221_6515.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000614 Genomic DNA. Translation: ABO55276.1 .
    RefSeqi YP_001120111.1. NC_009256.1.

    3D structure databases

    ProteinModelPortali A4JG73.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 269482.Bcep1808_2277.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABO55276 ; ABO55276 ; Bcep1808_2277 .
    GeneIDi 4954012.
    KEGGi bvi:Bcep1808_2277.
    PATRICi 19323128. VBIBurVie89221_6515.

    Phylogenomic databases

    eggNOGi COG0365.
    HOGENOMi HOG000229981.
    KOi K01895.
    OMAi AWIWYRD.
    OrthoDBi EOG68WR2H.

    Enzyme and pathway databases

    BioCyci BVIE269482:GJNA-2339-MONOMER.

    Family and domain databases

    HAMAPi MF_01123. Ac_CoA_synth.
    InterProi IPR011904. Ac_CoA_lig.
    IPR025110. AMP-bd_C.
    IPR020845. AMP-binding_CS.
    IPR000873. AMP-dep_Synth/Lig.
    [Graphical view ]
    Pfami PF00501. AMP-binding. 1 hit.
    PF13193. AMP-binding_C. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR02188. Ac_CoA_lig_AcsA. 1 hit.
    PROSITEi PS00455. AMP_BINDING. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of chromosome 1 of Burkholderia vietnamiensis G4."
      US DOE Joint Genome Institute
      Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M.
      , Hauser L., Kyrpides N., Tiedje J., Richardson P.
      Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: G4 / LMG 22486Imported.

    Entry informationi

    Entry nameiA4JG73_BURVG
    AccessioniPrimary (citable) accession number: A4JG73
    Entry historyi
    Integrated into UniProtKB/TrEMBL: May 1, 2007
    Last sequence update: May 1, 2007
    Last modified: October 1, 2014
    This is version 49 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteomeImported

    External Data

    Dasty 3