Reviewed, UniProtKB/Swiss-Prot A4JFY6 (KDSA_BURVG)

Names and origin

Protein names

Recommended name:
    2-dehydro-3-deoxyphosphooctonate aldolase
    EC=2.5.1.55
Alternative name(s):
    Phospho-2-dehydro-3-deoxyoctonate aldolase
    3-deoxy-D-manno-octulosonic acid 8-phosphate synthetase
    KDO-8-phosphate synthetase
      Short name=KDO 8-P synthase
      Short name=KDOPS

Gene names

Name:	kdsA
Ordered Locus Names:	Bcep1808_2187

Organism

Burkholderia vietnamiensis (strain G4 / LMG 22486) (Burkholderia cepacia (strain R1808)) [Complete proteome] [HAMAP]

Taxonomic identifier

269482 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	284 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + D-arabinose 5-phosphate + H2O = 2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate. HAMAP MF_00056
Pathway	Carbohydrate biosynthesis; 3-deoxy-D-manno-octulosonate biosynthesis; 3-deoxy-D-manno-octulosonate from D-ribulose 5-phosphate: step 2/3. HAMAP MF_00056 Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis. HAMAP MF_00056
Subcellular location	Cytoplasm By similarity HAMAP MF_00056.
Sequence similarities	Belongs to the kdsA family.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	keto-3-deoxy-D-manno-octulosonic acid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-deoxy-8-phosphooctulonate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 284	284	2-dehydro-3-deoxyphosphooctonate aldolase HAMAP MF_00056		PRO_1000003331

Sequences

	Sequence		Length	Mass (Da)
	A4JFY6-1 [UniParc]. Last modified May 1, 2007. Version 1. Checksum: BC0892671A4C98E6	FASTA	284	30,849
	10 20 30 40 50 60 MKLCDFEVGL DQPFFLIAGT CVVESEQMTI DTAGRLKEIC AKLNVPFIYK SSYDKANRSS 70 80 90 100 110 120 GKSFRGLGMD EGLRILGEVK RQLGLPVLTD VHSIDEIEQV ASVVDVLQTP AFLCRQTDFI 130 140 150 160 170 180 HACARSGKPV NIKKGQFLAP HDMKNVIDKA RDAAREAGLS EDRFMACERG VSFGYNNLVS 190 200 210 220 230 240 DMRSLAIMRE TNAPVVFDAT HSVQLPGGQG TSSGGQREFV PVLARAAVAT GVAGLFMETH 250 260 270 280 PNPAEAKSDG PNAVPLHRMG ALLETLVTLD QAVKRNPLLE NDFN

References

[1]

"Complete sequence of chromosome 1 of Burkholderia vietnamiensis G4." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Tiedje J., Richardson P. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000614 Genomic DNA. Translation: ABO55189.1.
RefSeq	YP_001120024.1.
3D structure databases
SMR	A4JFY6. Positions 12-276.
ModBase	Search...
Protein-protein interaction databases
STRING	A4JFY6.
Genome annotation databases
GeneID	4953918.
GenomeReviews	Gene locus Bcep1808_2187 in contig CP000614_GR.
KEGG	bvi:Bcep1808_2187.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2877.
HOGENOM	HBG575990.
OMA	LAPWDMG.
Family and domain databases
HAMAP	MF_00056. KDO8P_synth. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR006218. DAHP1/KDSA. IPR006269. KDO8P_synthase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR21057:SF2. KDO8P_synthase. 1 hit.
Pfam	PF00793. DAHP_synth_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR01362. KDO8P_synth. 1 hit.
ProtoNet	Search...

Entry information

Entry name

KDSA_BURVG

Accession

Primary (citable) accession number: A4JFY6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 1, 2007
Last modified:	February 9, 2010
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)