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A4JF78

- GLND_BURVG

UniProt

A4JF78 - GLND_BURVG

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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene
glnD, Bcep1808_1928
Organism
Burkholderia vietnamiensis (strain G4 / LMG 22486) (Burkholderia cepacia (strain R1808))
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen fixation and metabolism By similarity.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity By similarity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. metal ion binding Source: InterPro
  4. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. nitrogen fixation Source: UniProtKB-HAMAP
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

Nitrogen fixation

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciBVIE269482:GJNA-1978-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzyme
Short name:
UTase/UR
Alternative name(s):
Bifunctional [protein-PII] modification enzyme
Bifunctional nitrogen sensor protein
Including the following 2 domains:
[Protein-PII] uridylyltransferase (EC:2.7.7.59)
Short name:
PII uridylyltransferase
Short name:
UTase
[Protein-PII]-UMP uridylyl-removing enzyme (EC:3.1.4.-)
Short name:
UR
Gene namesi
Name:glnD
Ordered Locus Names:Bcep1808_1928
OrganismiBurkholderia vietnamiensis (strain G4 / LMG 22486) (Burkholderia cepacia (strain R1808))
Taxonomic identifieri269482 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex
ProteomesiUP000002287: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 859859Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotationPRO_1000022337Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi269482.Bcep1808_1928.

Structurei

3D structure databases

ProteinModelPortaliA4JF78.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini445 – 546102HDAdd
BLAST
Domaini683 – 76280ACT 1Add
BLAST
Domaini791 – 85969ACT 2Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 325325UridylyltransferaseUniRule annotationAdd
BLAST
Regioni326 – 682357Uridylyl-removingUniRule annotationAdd
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing By similarity.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.
Contains 2 ACT domains.
Contains 1 HD domain.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261778.
KOiK00990.
OMAiHHLLMSV.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 2 hits.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4JF78-1 [UniParc]FASTAAdd to Basket

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MSAHAAPSPE ALSRRAEFKA AKADLLERFR SATNVTSLMH ALSKLTDSAL    50
KRVWDDCGLP ATLALVAVGG YGRGELAPHS DVDILVLLPD AHDPALDARI 100
ERFIGMAWDL GLEIGSSVRT VAQCIEEASQ DVTVQTSLLE ARRIFGSTAL 150
FERFTVRYHE EALDARAFFT AKVLEMRQRH AKFQDTPYSL EPNVKESPGG 200
LRDLQTILWI ARAAGFGSSW RELDTRGLIT EREARELRRN EGFLKALRAR 250
LHVIAGRRQD VLVFDLQTQA AESFGYRPTQ AKRASEQLMR RYYWAAKAVT 300
QLATILIQNI EAQLFPATSG ITRVLSHDRF VEKQGMLEIV DDGVFERHPD 350
AILEAFLLYE TTRGVKGLSA RTLRALYNSR EIMNDTWRRD AQNRHTFMQI 400
LQQPEGITHA FRLMNQTSVL GRYLLNFRRI VGQMQHDLYH VYTVDQHILM 450
VLRNIRRFAV AEHAHEYPFC SQLIGNFERP WVLYVAALFH DIAKGRGGDH 500
STLGMADARR FCREHGIGGD DAALIVWLVQ HHLTMSQVAQ KQDTSDPEVI 550
KRFAAIVGNE RYLTALYLLT VADIRGTSPK VWNTWKGKLL EDLYRITLAV 600
LGGAKPDAHS ELKSRQEQAL ALLRLETVPD DAHRALWDQL DVGFFLRHDA 650
ADIAWQTRVL YRHVNAETAI VRARPSPIGD ALQVLVYVKD RPDLFAGICA 700
YFDRNGLSVL DARVSTTRHG YALDNFIVTQ TERDVRYRDI ANLVEQQLAT 750
RLAETAPLPE PSKGRLSRLS RTFPITPRVD LRADERGQYY ILSVSANDRP 800
GLLYSIARVL AEHRVGVHAA RINTLGERVE DIFLLDGAGL SDNRLQIQLE 850
TELLRAIAV 859
Length:859
Mass (Da):97,064
Last modified:May 1, 2007 - v1
Checksum:iAD7EF37E58B2FD88
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000614 Genomic DNA. Translation: ABO54931.1.
RefSeqiYP_001119766.1. NC_009256.1.

Genome annotation databases

EnsemblBacteriaiABO54931; ABO54931; Bcep1808_1928.
GeneIDi4953651.
KEGGibvi:Bcep1808_1928.
PATRICi19322457. VBIBurVie89221_6191.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000614 Genomic DNA. Translation: ABO54931.1 .
RefSeqi YP_001119766.1. NC_009256.1.

3D structure databases

ProteinModelPortali A4JF78.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 269482.Bcep1808_1928.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABO54931 ; ABO54931 ; Bcep1808_1928 .
GeneIDi 4953651.
KEGGi bvi:Bcep1808_1928.
PATRICi 19322457. VBIBurVie89221_6191.

Phylogenomic databases

eggNOGi COG2844.
HOGENOMi HOG000261778.
KOi K00990.
OMAi HHLLMSV.
OrthoDBi EOG6CCH44.

Enzyme and pathway databases

BioCyci BVIE269482:GJNA-1978-MONOMER.

Family and domain databases

Gene3Di 1.10.3210.10. 1 hit.
HAMAPi MF_00277. PII_uridylyl_transf.
InterProi IPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view ]
Pfami PF01842. ACT. 2 hits.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
PROSITEi PS51671. ACT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of chromosome 1 of Burkholderia vietnamiensis G4."
    US DOE Joint Genome Institute
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M.
    , Hauser L., Kyrpides N., Tiedje J., Richardson P.
    Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: G4 / LMG 22486.

Entry informationi

Entry nameiGLND_BURVG
AccessioniPrimary (citable) accession number: A4JF78
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 1, 2007
Last modified: June 11, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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