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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Burkholderia vietnamiensis (strain G4 / LMG 22486) (Burkholderia cepacia (strain R1808))
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen fixation and metabolism.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Multifunctional enzyme, Nucleotidyltransferase, Transferase
Biological processNitrogen fixation
LigandMagnesium

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
Short name:
UTase/URUniRule annotation
Alternative name(s):
Bifunctional [protein-PII] modification enzymeUniRule annotation
Bifunctional nitrogen sensor proteinUniRule annotation
Including the following 2 domains:
[Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
Short name:
PII uridylyltransferaseUniRule annotation
Short name:
UTaseUniRule annotation
[Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
Short name:
URUniRule annotation
Gene namesi
Name:glnDUniRule annotation
Ordered Locus Names:Bcep1808_1928
OrganismiBurkholderia vietnamiensis (strain G4 / LMG 22486) (Burkholderia cepacia (strain R1808))
Taxonomic identifieri269482 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex
Proteomesi
  • UP000002287 Componenti: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000223371 – 859Bifunctional uridylyltransferase/uridylyl-removing enzymeAdd BLAST859

Proteomic databases

PRIDEiA4JF78

Interactioni

Protein-protein interaction databases

STRINGi269482.Bcep1808_1928

Structurei

3D structure databases

ProteinModelPortaliA4JF78
SMRiA4JF78
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini444 – 566HDPROSITE-ProRule annotationAdd BLAST123
Domaini683 – 762ACT 1UniRule annotationAdd BLAST80
Domaini791 – 859ACT 2UniRule annotationAdd BLAST69

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 325UridylyltransferaseAdd BLAST325
Regioni326 – 682Uridylyl-removingAdd BLAST357

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105E1P Bacteria
COG2844 LUCA
HOGENOMiHOG000261778
KOiK00990
OMAiARICGYF
OrthoDBiPOG091H04HO

Family and domain databases

CDDicd00077 HDc, 1 hit
HAMAPiMF_00277 PII_uridylyl_transf, 1 hit
InterProiView protein in InterPro
IPR002912 ACT_dom
IPR003607 HD/PDEase_dom
IPR006674 HD_domain
IPR013546 PII_UdlTrfase/GS_AdlTrfase
IPR002934 Polymerase_NTP_transf_dom
IPR010043 UTase/UR
PANTHERiPTHR13734:SF45 PTHR13734:SF45, 2 hits
PfamiView protein in Pfam
PF08335 GlnD_UR_UTase, 1 hit
PF01966 HD, 1 hit
PF01909 NTP_transf_2, 1 hit
PIRSFiPIRSF006288 PII_uridyltransf, 1 hit
SMARTiView protein in SMART
SM00471 HDc, 1 hit
TIGRFAMsiTIGR01693 UTase_glnD, 1 hit
PROSITEiView protein in PROSITE
PS51671 ACT, 2 hits
PS51831 HD, 1 hit

Sequencei

Sequence statusi: Complete.

A4JF78-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAHAAPSPE ALSRRAEFKA AKADLLERFR SATNVTSLMH ALSKLTDSAL
60 70 80 90 100
KRVWDDCGLP ATLALVAVGG YGRGELAPHS DVDILVLLPD AHDPALDARI
110 120 130 140 150
ERFIGMAWDL GLEIGSSVRT VAQCIEEASQ DVTVQTSLLE ARRIFGSTAL
160 170 180 190 200
FERFTVRYHE EALDARAFFT AKVLEMRQRH AKFQDTPYSL EPNVKESPGG
210 220 230 240 250
LRDLQTILWI ARAAGFGSSW RELDTRGLIT EREARELRRN EGFLKALRAR
260 270 280 290 300
LHVIAGRRQD VLVFDLQTQA AESFGYRPTQ AKRASEQLMR RYYWAAKAVT
310 320 330 340 350
QLATILIQNI EAQLFPATSG ITRVLSHDRF VEKQGMLEIV DDGVFERHPD
360 370 380 390 400
AILEAFLLYE TTRGVKGLSA RTLRALYNSR EIMNDTWRRD AQNRHTFMQI
410 420 430 440 450
LQQPEGITHA FRLMNQTSVL GRYLLNFRRI VGQMQHDLYH VYTVDQHILM
460 470 480 490 500
VLRNIRRFAV AEHAHEYPFC SQLIGNFERP WVLYVAALFH DIAKGRGGDH
510 520 530 540 550
STLGMADARR FCREHGIGGD DAALIVWLVQ HHLTMSQVAQ KQDTSDPEVI
560 570 580 590 600
KRFAAIVGNE RYLTALYLLT VADIRGTSPK VWNTWKGKLL EDLYRITLAV
610 620 630 640 650
LGGAKPDAHS ELKSRQEQAL ALLRLETVPD DAHRALWDQL DVGFFLRHDA
660 670 680 690 700
ADIAWQTRVL YRHVNAETAI VRARPSPIGD ALQVLVYVKD RPDLFAGICA
710 720 730 740 750
YFDRNGLSVL DARVSTTRHG YALDNFIVTQ TERDVRYRDI ANLVEQQLAT
760 770 780 790 800
RLAETAPLPE PSKGRLSRLS RTFPITPRVD LRADERGQYY ILSVSANDRP
810 820 830 840 850
GLLYSIARVL AEHRVGVHAA RINTLGERVE DIFLLDGAGL SDNRLQIQLE

TELLRAIAV
Length:859
Mass (Da):97,064
Last modified:May 1, 2007 - v1
Checksum:iAD7EF37E58B2FD88
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000614 Genomic DNA Translation: ABO54931.1

Genome annotation databases

EnsemblBacteriaiABO54931; ABO54931; Bcep1808_1928
KEGGibvi:Bcep1808_1928

Similar proteinsi

Entry informationi

Entry nameiGLND_BURVG
AccessioniPrimary (citable) accession number: A4JF78
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 1, 2007
Last modified: May 23, 2018
This is version 80 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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