Reviewed, UniProtKB/Swiss-Prot A4JBI4 (PROA_BURVG)

Names and origin

Protein names

Recommended name:
    Gamma-glutamyl phosphate reductase
      Short name=GPR
    EC=1.2.1.41
Alternative name(s):
    Glutamate-5-semialdehyde dehydrogenase
    Glutamyl-gamma-semialdehyde dehydrogenase
      Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	Bcep1808_0625

Organism

Burkholderia vietnamiensis (strain G4 / LMG 22486) (Burkholderia cepacia (strain R1808)) [Complete proteome] [HAMAP]

Taxonomic identifier

269482 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	423 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate.
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP(+) = L-glutamyl 5-phosphate + NADPH.
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 423	423	Gamma-glutamyl phosphate reductase		PRO_1000049940

Sequences

	Sequence		Length	Mass (Da)
	A4JBI4-1 [UniParc]. Last modified May 1, 2007. Version 1. Checksum: FBD0CEB57F5E8584	FASTA	423	45,062
	10 20 30 40 50 60 MDIDQYMTDL GRRARHASRA MARASTAAKN AALDAVARAI ERDASALKEA NARDVARARD 70 80 90 100 110 120 KGLDAAFIDR LTLSDKALKT MVEGLRQVAS LADPIGEISN LKYRPSGIQV GQMRVPLGVI 130 140 150 160 170 180 GIIYESRPNV TIDAAALCLK SGNATILRGG SEALESNAAL AKLIGEGLEA AGLPQDAVQV 190 200 210 220 230 240 VATADRAAVG KLITMTEYVD VIVPRGGKSL IERLINEARV PMIKHLDGIC HVYVDDRADL 250 260 270 280 290 300 AKALTVCDNA KTHRYGTCNT METLLVSSGI AAALLPPLGK LYRDKQVELR VDAAARAVLA 310 320 330 340 350 360 DAGVGPLVDA TDADWHTEYL APVLAIKVVD GLDAAIEHIN HYGSHHTDAI VTEDHDRAMR 370 380 390 400 410 420 FLREVDSASV MVNASTRFAD GFEFGLGAEI GISNDKLHAR GPVGLEGLTS LKYVVLGHGE GRQ

References

[1]

"Complete sequence of chromosome 1 of Burkholderia vietnamiensis G4." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Tiedje J., Richardson P. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000614 Genomic DNA. Translation: ABO53637.1.
RefSeq	YP_001118472.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4952319.
GenomeReviews	Gene locus Bcep1808_0625 in contig CP000614_GR.
KEGG	bvi:Bcep1808_0625.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00412. [Tree]
InterPro	IPR016163. Ald_DHase_C. IPR016162. Ald_DHase_N. IPR015590. Aldehyde_DHase. IPR000965. Gglut_pp_reduct. IPR012134. Glu-5-SA_DHase. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11063:SF1. GSA_DH. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PIRSF	PIRSF000151. GPR. 1 hit.
TIGRFAMs	TIGR00407. proA. 1 hit.
PROSITE	PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_BURVG

Accession

Primary (citable) accession number: A4JBI4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 1, 2007
Last modified:	November 25, 2008
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)