A4JBB9 (PROB_BURVG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate 5-kinase
EC=2.7.2.11

Alternative name(s):
Gamma-glutamyl kinase
Short name=GK

Gene names

Name:	proB
Ordered Locus Names:	Bcep1808_0560

Organism

Burkholderia vietnamiensis (strain G4 / LMG 22486) (Burkholderia cepacia (strain R1808)) [Complete proteome] [HAMAP]

Taxonomic identifier

269482 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	372 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline By similarity. HAMAP MF_00456
Catalytic activity	ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP MF_00456
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP MF_00456
Subcellular location	Cytoplasm By similarity HAMAP MF_00456.
Sequence similarities	Belongs to the glutamate 5-kinase family. Contains 1 PUA domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro glutamate 5-kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 372	372	Glutamate 5-kinase HAMAP MF_00456		PRO_1000081047
Regions
	Domain	280 – 358	79	PUA

Sequences

	Sequence		Length	Mass (Da)
	A4JBB9 [UniParc]. Last modified May 1, 2007. Version 1. Checksum: 6B5B5C1D9026D41E	FASTA	372	39,435
	10 20 30 40 50 60 MRSIIADSKR LVVKVGSSLV TNDGKGLDHA AIGRWAAQIA ALRAQGKEVV LVSSGAIAEG 70 80 90 100 110 120 MQRLGWNKRP REIDELQAAA AVGQMGLAQV YESRFTEHHI RTAQILLTHA DLADRERYLN 130 140 150 160 170 180 ARSTLLTLLR LGVVPIINEN DTVVTDEIKF GDNDTLGALV ANLIEGDALI ILTDQTGLFT 190 200 210 220 230 240 ADPRKDPNAT LVAEASAGAP ELESMAGGAG SSLGRGGMLT KILAAKRAAH SGANTVIASG 250 260 270 280 290 300 READVLVRLA AGEAIGTQLI ARTARMAARK QWMADHLQVR GHVVIDAGAV EKLRDGGKSL 310 320 330 340 350 360 LPIGVIDVQG AFARGEVIAC VGPDGREVAR GLTNYSSAET KLIHRKPSGE IETVLGYMLE 370 PELIHRDNLV LV

References

[1]

"Complete sequence of chromosome 1 of Burkholderia vietnamiensis G4." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Tiedje J., Richardson P. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: G4 / LMG 22486.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000614 Genomic DNA. Translation: ABO53572.1.
RefSeq	YP_001118407.1. NC_009256.1.
3D structure databases
ProteinModelPortal	A4JBB9.
ModBase	Search...
Protein-protein interaction databases
STRING	A4JBB9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4952254.
GenomeReviews	Gene locus Bcep1808_0560 in contig CP000614_GR.
KEGG	bvi:Bcep1808_0560.
PATRIC	19319536. VBIBurVie89221_4759.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0263.
HOGENOM	HBG507643.
OMA	TFGDNDM.
ProtClustDB	PRK05429.
Enzyme and pathway databases
BioCyc	BVIE269482:BCEP1808_0560-MONOMER.
Family and domain databases
HAMAP	MF_00456. ProB. [Tree]
InterPro	IPR001048. Asp/Glu/Uridylate_kinase. IPR001057. Glu/AcGlu_kinase. IPR011529. Glu_5kinase. IPR005715. Glu_5kinase/COase_Synthase. IPR019797. Glutamate_5-kinase_CS. IPR002478. PUA. IPR015947. PUA-like_domain. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
KO	K00931.
Pfam	PF00696. AA_kinase. 1 hit. PF01472. PUA. 1 hit. [Graphical view]
PIRSF	PIRSF000729. GK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
SMART	SM00359. PUA. 1 hit. [Graphical view]
SUPFAM	SSF53633. Aa_kinase. 1 hit. SSF88697. PUA-like. 1 hit.
TIGRFAMs	TIGR01027. ProB. 1 hit.
PROSITE	PS00902. GLUTAMATE_5_KINASE. 1 hit. PS50890. PUA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROB_BURVG

Accession

Primary (citable) accession number: A4JBB9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	May 1, 2007
Last modified:	December 14, 2011
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program