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Protein

Glycerol kinase

Gene

glpK

Organism
Desulfotomaculum reducens (strain MI-1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Key enzyme in the regulation of glycerol uptake and metabolism. Catalyzes the phosphorylation of glycerol to yield sn-glycerol 3-phosphate.UniRule annotation

Catalytic activityi

ATP + glycerol = ADP + sn-glycerol 3-phosphate.UniRule annotation

Enzyme regulationi

Activated by phosphorylation and inhibited by fructose 1,6-bisphosphate (FBP).UniRule annotation

Pathwayi: glycerol degradation via glycerol kinase pathway

This protein is involved in step 1 of the subpathway that synthesizes sn-glycerol 3-phosphate from glycerol.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Glycerol kinase (glpK)
This subpathway is part of the pathway glycerol degradation via glycerol kinase pathway, which is itself part of Polyol metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sn-glycerol 3-phosphate from glycerol, the pathway glycerol degradation via glycerol kinase pathway and in Polyol metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei14SubstrateUniRule annotation1
Binding sitei18ATPUniRule annotation1
Binding sitei136SubstrateUniRule annotation1
Binding sitei268ATPUniRule annotation1
Binding sitei311ATP; via carbonyl oxygenUniRule annotation1
Binding sitei315ATP; via amide nitrogenUniRule annotation1
Binding sitei330ATPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi14 – 16ATPUniRule annotation3
Nucleotide bindingi412 – 416ATPUniRule annotation5

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycerol metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00618; UER00672.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol kinaseUniRule annotation (EC:2.7.1.30UniRule annotation)
Alternative name(s):
ATP:glycerol 3-phosphotransferaseUniRule annotation
GlycerokinaseUniRule annotation
Short name:
GKUniRule annotation
Gene namesi
Name:glpKUniRule annotation
Ordered Locus Names:Dred_2845
OrganismiDesulfotomaculum reducens (strain MI-1)
Taxonomic identifieri349161 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesPeptococcaceaeDesulfotomaculum
Proteomesi
  • UP000001556 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10002039511 – 501Glycerol kinaseAdd BLAST501

Proteomic databases

PRIDEiA4J8E6.

Interactioni

Subunit structurei

Homotetramer and homodimer (in equilibrium).UniRule annotation

Protein-protein interaction databases

STRINGi349161.Dred_2845.

Structurei

3D structure databases

ProteinModelPortaliA4J8E6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni84 – 85Substrate bindingUniRule annotation2
Regioni246 – 247Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the FGGY kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108HMR. Bacteria.
COG0554. LUCA.
HOGENOMiHOG000222134.
KOiK00864.
OMAiPMEIFAT.
OrthoDBiPOG091H02FA.

Family and domain databases

HAMAPiMF_00186. Glycerol_kin. 1 hit.
InterProiIPR000577. Carb_kinase_FGGY.
IPR018485. Carb_kinase_FGGY_C.
IPR018483. Carb_kinase_FGGY_CS.
IPR018484. Carb_kinase_FGGY_N.
IPR005999. Glycerol_kin.
[Graphical view]
PfamiPF02782. FGGY_C. 1 hit.
PF00370. FGGY_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000538. GlpK. 1 hit.
TIGRFAMsiTIGR01311. glycerol_kin. 1 hit.
PROSITEiPS00933. FGGY_KINASES_1. 1 hit.
PS00445. FGGY_KINASES_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4J8E6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMAKKYVLAL DQGTTSCRAI LFDRDSNIVG VAQKEFTQIY PKPGWVEHNA
60 70 80 90 100
EEIWSTQYGV IAEVVAKTGI KPEEIASIGI TNQRETTVVW NKNTGKPVYN
110 120 130 140 150
AIVWQCRRTT EICDELKVKG LEETFKKKTG LVVDAYFSGT KVKWILDNVP
160 170 180 190 200
GTREMAEKGE LLFGTMDTWL IWKLTGGQVH VTDYSNASRT LMYNIRELCW
210 220 230 240 250
DEELLGYLNV PMSLLPTVKA SSEVYGYTQP DQFLGHSVPI AGVAGDQQAA
260 270 280 290 300
LFGQACYEPG MAKNTYGTGC FMLMNTGDEV YESKNGLLTT IAWGLDGKVT
310 320 330 340 350
YALEGSIFIA GAAIQWLRDG LKILESAPDS EYFASKVDGT DGVYLVPAFA
360 370 380 390 400
GLGAPYWDMR ARGAIVGLTR GTTKEHIIRA ALDSLAYQTK DVLGAMEADS
410 420 430 440 450
NIKLQALKVD GGAVANNLLM QFQADILGVP VERPQVIETT ALGAAYLAGL
460 470 480 490 500
AVGFWESKEE LAKRWKLDYQ FESVMAEDQR NKLYNGWEKA VGRSMDWATE

E
Length:501
Mass (Da):55,336
Last modified:May 1, 2007 - v1
Checksum:i8199501E6061F226
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000612 Genomic DNA. Translation: ABO51349.1.

Genome annotation databases

EnsemblBacteriaiABO51349; ABO51349; Dred_2845.
KEGGidrm:Dred_2845.
PATRICi21732143. VBIDesRed82656_3116.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000612 Genomic DNA. Translation: ABO51349.1.

3D structure databases

ProteinModelPortaliA4J8E6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi349161.Dred_2845.

Proteomic databases

PRIDEiA4J8E6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABO51349; ABO51349; Dred_2845.
KEGGidrm:Dred_2845.
PATRICi21732143. VBIDesRed82656_3116.

Phylogenomic databases

eggNOGiENOG4108HMR. Bacteria.
COG0554. LUCA.
HOGENOMiHOG000222134.
KOiK00864.
OMAiPMEIFAT.
OrthoDBiPOG091H02FA.

Enzyme and pathway databases

UniPathwayiUPA00618; UER00672.

Family and domain databases

HAMAPiMF_00186. Glycerol_kin. 1 hit.
InterProiIPR000577. Carb_kinase_FGGY.
IPR018485. Carb_kinase_FGGY_C.
IPR018483. Carb_kinase_FGGY_CS.
IPR018484. Carb_kinase_FGGY_N.
IPR005999. Glycerol_kin.
[Graphical view]
PfamiPF02782. FGGY_C. 1 hit.
PF00370. FGGY_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000538. GlpK. 1 hit.
TIGRFAMsiTIGR01311. glycerol_kin. 1 hit.
PROSITEiPS00933. FGGY_KINASES_1. 1 hit.
PS00445. FGGY_KINASES_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGLPK_DESRM
AccessioniPrimary (citable) accession number: A4J8E6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: May 1, 2007
Last modified: November 2, 2016
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.