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A4J7H2 (SYL_DESRM) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Dred_2515
OrganismDesulfotomaculum reducens (strain MI-1) [Complete proteome] [HAMAP]
Taxonomic identifier349161 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesPeptococcaceaeDesulfotomaculum

Protein attributes

Sequence length827 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 827827Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000334750

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif581 – 5855"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5841ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A4J7H2 [UniParc].

Last modified May 1, 2007. Version 1.
Checksum: 5406E91E3D9A354D

FASTA82794,352
        10         20         30         40         50         60 
MQEHYDFKEI EKKWQEDWNK ENTYQVPDFS ERPKYYCLEM FPYPSGKLHM GHVRNYSIGD 

        70         80         90        100        110        120 
VVARFKTMQG YDVLHPMGWD AFGLPAENAA IKHGIAPATW TWDNIAHMRS QLKQLGLSYD 

       130        140        150        160        170        180 
WNREVATCHT EYYKWGQWLF LQLYKKGLCY KKHARVNWCP DCATVLANEQ VVDGACERCS 

       190        200        210        220        230        240 
AVVEQKELDQ WFFRITEYAQ RLLNDLKLLK GWPDKVKIMQ ENWIGRSEGA ELTFQVEGSD 

       250        260        270        280        290        300 
ESITVFTTRP DTVYGVSYMV LAPEHPLVAK LIAGRPQEAE VIGFVNRVNK LTELDRTSSD 

       310        320        330        340        350        360 
KEGVFTGAYC INPFDGARVP ILIANYVLYH YGTGAVMGVP AHDERDFEFA HKYNLPIKVV 

       370        380        390        400        410        420 
IYPAQDKEIR VEDMKEAYTA DGIMVHSGPF DGSPNRQGIK KVIKYAEEKG IGKGIVNYRL 

       430        440        450        460        470        480 
RDWLISRQRY WGTPIPIVYC EKCGTVPVPE DQLPVILPTD VAFKPTGESP LKGRPDFVHT 

       490        500        510        520        530        540 
TCPQCGGPAQ RETDTMDTFV DSSWYYLRYT SSRDTEYAWD KNKADRWMNV DQYIGGVEHA 

       550        560        570        580        590        600 
ILHLLYSRFF TKVFYDLGLV NVQEPFENLL TQGMVLKDGS KMSKSKGNVV SPEEIIDRYG 

       610        620        630        640        650        660 
ADTARMFILF AAPPERDLEW SDRGVEGSHR FLNRVWRLVY SLKDEVAGAP AISSVNAYVG 

       670        680        690        700        710        720 
VHKEMRRLTH YAIKKVTEDV SGRFNFNTAI STIMELVNGI YTYRDKVAAV ERDTAVLAEA 

       730        740        750        760        770        780 
VNSTIILLAP FAPHIAEELW SATGHQGSVH KQPWLTFDSA ALVEDEVEVA VQINGKVRER 

       790        800        810        820 
LNIPANMKPA EMQQYLMDME SVKILIGDKQ IVKIIPVPGK LLNIVVK 

« Hide

References

[1]"Complete sequence of Desulfotomaculum reducens MI-1."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Han C., Tapia R., Schmutz J., Larimer F. expand/collapse author list , Land M., Hauser L., Kyrpides N., Kim E., Tebo B.M., Richardson P.
Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: MI-1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000612 Genomic DNA. Translation: ABO51025.1.
RefSeqYP_001113850.1. NC_009253.1.

3D structure databases

ProteinModelPortalA4J7H2.
SMRA4J7H2. Positions 3-762.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING349161.Dred_2515.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABO51025; ABO51025; Dred_2515.
GeneID4956110.
KEGGdrm:Dred_2515.
PATRIC21731389. VBIDesRed82656_2751.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMADKPKYYA.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycDRED349161:GHP6-2586-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_DESRM
AccessionPrimary (citable) accession number: A4J7H2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 1, 2007
Last modified: May 14, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries