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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Desulfotomaculum reducens (strain MI-1)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei584 – 5841ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciDRED349161:GHP6-2586-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Dred_2515
OrganismiDesulfotomaculum reducens (strain MI-1)
Taxonomic identifieri349161 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesPeptococcaceaeDesulfotomaculum
ProteomesiUP000001556 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 827827Leucine--tRNA ligasePRO_0000334750Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi349161.Dred_2515.

Structurei

3D structure databases

ProteinModelPortaliA4J7H2.
SMRiA4J7H2. Positions 3-762.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi42 – 5211"HIGH" regionAdd
BLAST
Motifi581 – 5855"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4J7H2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQEHYDFKEI EKKWQEDWNK ENTYQVPDFS ERPKYYCLEM FPYPSGKLHM
60 70 80 90 100
GHVRNYSIGD VVARFKTMQG YDVLHPMGWD AFGLPAENAA IKHGIAPATW
110 120 130 140 150
TWDNIAHMRS QLKQLGLSYD WNREVATCHT EYYKWGQWLF LQLYKKGLCY
160 170 180 190 200
KKHARVNWCP DCATVLANEQ VVDGACERCS AVVEQKELDQ WFFRITEYAQ
210 220 230 240 250
RLLNDLKLLK GWPDKVKIMQ ENWIGRSEGA ELTFQVEGSD ESITVFTTRP
260 270 280 290 300
DTVYGVSYMV LAPEHPLVAK LIAGRPQEAE VIGFVNRVNK LTELDRTSSD
310 320 330 340 350
KEGVFTGAYC INPFDGARVP ILIANYVLYH YGTGAVMGVP AHDERDFEFA
360 370 380 390 400
HKYNLPIKVV IYPAQDKEIR VEDMKEAYTA DGIMVHSGPF DGSPNRQGIK
410 420 430 440 450
KVIKYAEEKG IGKGIVNYRL RDWLISRQRY WGTPIPIVYC EKCGTVPVPE
460 470 480 490 500
DQLPVILPTD VAFKPTGESP LKGRPDFVHT TCPQCGGPAQ RETDTMDTFV
510 520 530 540 550
DSSWYYLRYT SSRDTEYAWD KNKADRWMNV DQYIGGVEHA ILHLLYSRFF
560 570 580 590 600
TKVFYDLGLV NVQEPFENLL TQGMVLKDGS KMSKSKGNVV SPEEIIDRYG
610 620 630 640 650
ADTARMFILF AAPPERDLEW SDRGVEGSHR FLNRVWRLVY SLKDEVAGAP
660 670 680 690 700
AISSVNAYVG VHKEMRRLTH YAIKKVTEDV SGRFNFNTAI STIMELVNGI
710 720 730 740 750
YTYRDKVAAV ERDTAVLAEA VNSTIILLAP FAPHIAEELW SATGHQGSVH
760 770 780 790 800
KQPWLTFDSA ALVEDEVEVA VQINGKVRER LNIPANMKPA EMQQYLMDME
810 820
SVKILIGDKQ IVKIIPVPGK LLNIVVK
Length:827
Mass (Da):94,352
Last modified:May 1, 2007 - v1
Checksum:i5406E91E3D9A354D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000612 Genomic DNA. Translation: ABO51025.1.
RefSeqiWP_011878823.1. NC_009253.1.
YP_001113850.1. NC_009253.1.

Genome annotation databases

EnsemblBacteriaiABO51025; ABO51025; Dred_2515.
KEGGidrm:Dred_2515.
PATRICi21731389. VBIDesRed82656_2751.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000612 Genomic DNA. Translation: ABO51025.1.
RefSeqiWP_011878823.1. NC_009253.1.
YP_001113850.1. NC_009253.1.

3D structure databases

ProteinModelPortaliA4J7H2.
SMRiA4J7H2. Positions 3-762.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi349161.Dred_2515.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABO51025; ABO51025; Dred_2515.
KEGGidrm:Dred_2515.
PATRICi21731389. VBIDesRed82656_2751.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciDRED349161:GHP6-2586-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MI-1.

Entry informationi

Entry nameiSYL_DESRM
AccessioniPrimary (citable) accession number: A4J7H2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 1, 2007
Last modified: May 27, 2015
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.