A4J7D0 (SYGA_DESRM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycine--tRNA ligase alpha subunit
EC=6.1.1.14

Alternative name(s):
Glycyl-tRNA synthetase alpha subunit
Short name=GlyRS

Gene names

Name:	glyQ
Ordered Locus Names:	Dred_2473

Organism

Desulfotomaculum reducens (strain MI-1) [Complete proteome] [HAMAP]

Taxonomic identifier

349161 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Peptococcaceae › Desulfotomaculum

Protein attributes

Sequence length	295 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly). HAMAP MF_00254
Subunit structure	Tetramer of two alpha and two beta subunits By similarity.
Subcellular location	Cytoplasm HAMAP MF_00254.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glycine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 295	295	Glycine--tRNA ligase alpha subunit HAMAP MF_00254		PRO_1000071876

Sequences

	Sequence		Length	Mass (Da)
	A4J7D0 [UniParc]. Last modified May 1, 2007. Version 1. Checksum: 5372682A9D910936	FASTA	295	33,838
	10 20 30 40 50 60 MNFQELILTL NKFWAEQNCI IQQPYDIEKG AGTMNPATFL RALGPEPWRV AYVEPSRRPT 70 80 90 100 110 120 DGRYGENPNR LQHYFQYQVI LKPSPDDVIP VYLDSLRAIG IDPDKHDIRF VEDNWESPTL 130 140 150 160 170 180 GAWGLGWEVW LDGMEVTQFT YFQQCGGIDC HPVSAEITYG LERLAMFIQQ KDSVYDITWV 190 200 210 220 230 240 GDITYGDVYH QNEVEQSGYN FEVANTEMLF DLFDMYEAEA NHILEKGLVL PAYDYVLKCS 250 260 270 280 290 HTFNLLDARG AISVSERQGF IARVRQMARA CAHAYVEQRE KLGFPLLKKG GNING

References

[1]

"Complete sequence of Desulfotomaculum reducens MI-1." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Han C., Tapia R., Schmutz J., Larimer F. , Land M., Hauser L., Kyrpides N., Kim E., Tebo B.M., Richardson P. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MI-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000612 Genomic DNA. Translation: ABO50983.1.
RefSeq	YP_001113808.1. NC_009253.1.
3D structure databases
ProteinModelPortal	A4J7D0.
SMR	A4J7D0. Positions 1-277.
ModBase	Search...
Protein-protein interaction databases
STRING	A4J7D0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4957152.
GenomeReviews	Gene locus Dred_2473 in contig CP000612_GR.
KEGG	drm:Dred_2473.
PATRIC	21731301. VBIDesRed82656_2707.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0752.
HOGENOM	HBG285316.
OMA	CRRPTDG.
PhylomeDB	A4J7D0.
ProtClustDB	PRK09348.
Enzyme and pathway databases
BioCyc	DRED349161:DRED_2473-MONOMER.
Family and domain databases
HAMAP	MF_00254. Gly_tRNA_synth_alpha. [Tree]
InterPro	IPR006194. Gly-tRNA-synth_II_heterodimer. IPR002310. Gly-tRNA_synth_IIc_asu. [Graphical view]
KO	K01878.
Pfam	PF02091. tRNA-synt_2e. 1 hit. [Graphical view]
PRINTS	PR01044. TRNASYNTHGA.
TIGRFAMs	TIGR00388. GlyQ. 1 hit.
PROSITE	PS50861. AA_TRNA_LIGASE_II_GLYAB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYGA_DESRM

Accession

Primary (citable) accession number: A4J7D0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	May 1, 2007
Last modified:	January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program