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Protein

Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO

Gene

trmFO

Organism
Desulfotomaculum reducens (strain MI-1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + uracil(54) in tRNA + FADH2 = tetrahydrofolate + 5-methyluracil(54) in tRNA + FAD.UniRule annotation

Cofactori

FADUniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 15FADUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processtRNA processing
LigandFAD, Flavoprotein

Names & Taxonomyi

Protein namesi
Recommended name:
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOUniRule annotation (EC:2.1.1.74UniRule annotation)
Alternative name(s):
Folate-dependent tRNA (uracil-5-)-methyltransferaseUniRule annotation
Folate-dependent tRNA(M-5-U54)-methyltransferaseUniRule annotation
Gene namesi
Name:trmFOUniRule annotation
Synonyms:gid
Ordered Locus Names:Dred_1983
OrganismiDesulfotomaculum reducens (strain MI-1)
Taxonomic identifieri349161 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesPeptococcaceaeDesulfotomaculum
Proteomesi
  • UP000001556 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000842861 – 441Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOAdd BLAST441

Interactioni

Protein-protein interaction databases

STRINGi349161.Dred_1983.

Structurei

3D structure databases

ProteinModelPortaliA4J5Z8.
SMRiA4J5Z8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MnmG family. TrmFO subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108FW6. Bacteria.
COG1206. LUCA.
HOGENOMiHOG000252054.
KOiK04094.
OMAiDYLNCPM.
OrthoDBiPOG091808QD.

Family and domain databases

Gene3Di3.50.50.60. 4 hits.
HAMAPiMF_01037. TrmFO. 1 hit.
InterProiView protein in InterPro
IPR023753. FAD/NAD-binding_dom.
IPR002218. MnmG-rel.
IPR020595. MnmG-rel_CS.
IPR004417. TrmFO.
PfamiView protein in Pfam
PF01134. GIDA. 1 hit.
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.
PROSITEiView protein in PROSITE
PS01281. GIDA_2. 1 hit.

Sequencei

Sequence statusi: Complete.

A4J5Z8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEHRVTVIG AGLAGSEAAW QLARRGIHVD LYEMRPQKYP PAHHTAYFSE
60 70 80 90 100
LVCSNSLRAA AIENAVGLLK EEMRQLDSLI ISAADNHKVP AGGALAVDRE
110 120 130 140 150
GFSRYITETL EDHPLIDIHR EEVTKIPEKG IVIVASGPLT SESLSHEIAK
160 170 180 190 200
VTGEQYLYFY DAAAPIVTLE SLDMTKVFRA SRYGKGEEAY LNCPMDQDEY
210 220 230 240 250
EKFYDALIHA ERAPIKEFEK QVHFEGCMPV EVLASRGKDT LLYGPLKPVG
260 270 280 290 300
LTDPRTGKRP HGVVQLRQDN AEGTLFNLVG FQTNLKWGEQ KRVFSFIPGL
310 320 330 340 350
EDAEFVRYGV MHRNTYITSP VLLQPTLQMK EHPRIFFAGQ ITGVEGYVES
360 370 380 390 400
AAAGLIAGIN AAALAKQKDL LIFPKETAHG ALMHYITTAS TKNFQPMNVT
410 420 430 440
FGLFPELTVR LKGKRERGRA QAERALEILK EWIEKEKICD I
Length:441
Mass (Da):49,236
Last modified:May 1, 2007 - v1
Checksum:iE14CF3D541C00481
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000612 Genomic DNA. Translation: ABO50501.1.
RefSeqiWP_011878311.1. NC_009253.1.

Genome annotation databases

EnsemblBacteriaiABO50501; ABO50501; Dred_1983.
KEGGidrm:Dred_1983.
PATRICi21730203. VBIDesRed82656_2174.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000612 Genomic DNA. Translation: ABO50501.1.
RefSeqiWP_011878311.1. NC_009253.1.

3D structure databases

ProteinModelPortaliA4J5Z8.
SMRiA4J5Z8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi349161.Dred_1983.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABO50501; ABO50501; Dred_1983.
KEGGidrm:Dred_1983.
PATRICi21730203. VBIDesRed82656_2174.

Phylogenomic databases

eggNOGiENOG4108FW6. Bacteria.
COG1206. LUCA.
HOGENOMiHOG000252054.
KOiK04094.
OMAiDYLNCPM.
OrthoDBiPOG091808QD.

Family and domain databases

Gene3Di3.50.50.60. 4 hits.
HAMAPiMF_01037. TrmFO. 1 hit.
InterProiView protein in InterPro
IPR023753. FAD/NAD-binding_dom.
IPR002218. MnmG-rel.
IPR020595. MnmG-rel_CS.
IPR004417. TrmFO.
PfamiView protein in Pfam
PF01134. GIDA. 1 hit.
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.
PROSITEiView protein in PROSITE
PS01281. GIDA_2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTRMFO_DESRM
AccessioniPrimary (citable) accession number: A4J5Z8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 1, 2007
Last modified: February 15, 2017
This is version 67 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.