A4J5C3 (METE_DESRM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
EC=2.1.1.14

Alternative name(s):
Cobalamin-independent methionine synthase
Methionine synthase, vitamin-B12 independent isozyme

Gene names

Name:	metE
Ordered Locus Names:	Dred_1751

Organism

Desulfotomaculum reducens (strain MI-1) [Complete proteome] [HAMAP]

Taxonomic identifier

349161 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Peptococcaceae › Desulfotomaculum

Protein attributes

Sequence length	764 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 764	764	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_1000071611
Sites
	Metal binding	645	1	Zinc By similarity
	Metal binding	647	1	Zinc By similarity
	Metal binding	730	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4J5C3 [UniParc]. Last modified May 1, 2007. Version 1. Checksum: 9BDE4463208CC172	FASTA	764	88,373
	10 20 30 40 50 60 MYTGATTYVV GFPRIGEQRE LKKVLERYWD QKTSFSEIDE IAKNLRRRHW LYQKEAGISF 70 80 90 100 110 120 ISSNDFSLYD NMLDTAIMLN AVPERFSNIH DKQELYFAMA RGTNNTVAME MTKWFNTNYH 130 140 150 160 170 180 YIVPELSDTM NFSLCADKII NEYREAKEYG LRTKINLIGP ITFLSLSKPV DGNQDTLELL 190 200 210 220 230 240 PKILPCYVSL LKKIADLDDE VYIQFDEPIV VKDIDRRTLN LFYLCYKELS NVSNRLRLIV 250 260 270 280 290 300 MTYFDHAVEA VKILSDIPIY GIGLDFVYGQ ENLKVLDSLN GKKLIAGVID GRNIWKNNYH 310 320 330 340 350 360 NTLTLLKDIE KNVKKEDIIL STTCSLLHVP YSLKHENHLD KDIKSWMSFA REKLDELSDL 370 380 390 400 410 420 AKLFFVENNS EDILTILENN QRIFREKQQS SKVIDPIVRE RVGNIKRRER DGSFNDRNEA 430 440 450 460 470 480 QRKKLDLPLL PTTTIGSFPQ TEEIRKLRRD FKNNVISQIE YDTGIKNYID SCIQFQEDIG 490 500 510 520 530 540 LDVLVHGEPE RNDMVEYFGE RLSGFVFTQN GWVQSYGSRC VKPTVIFGDV SRPKPMTIDT 550 560 570 580 590 600 ILYAKSKTNK IVKGMLTGPV TILNWSYCRS DMERSAVCEQ IALAIRDEIN DLQKAGIKII 610 620 630 640 650 660 QVDEAAFKEG YPLRKEKVAY YENWAVKSFK LAVSSAAIET QIHTHMCYSD FNDIIHTIEK 670 680 690 700 710 720 MDADVITIET SRSGNKLLTV FATRGYKNEI GPGIYDIHSP RVPSVEELEE KIRNLMLVLP 730 740 750 760 PSKLWINPDC GLKTRKWDEI RWSLSNMVKA AQHIRLGLQR VMFV

References

[1]

"Complete sequence of Desulfotomaculum reducens MI-1." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Han C., Tapia R., Schmutz J., Larimer F. , Land M., Hauser L., Kyrpides N., Kim E., Tebo B.M., Richardson P. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MI-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000612 Genomic DNA. Translation: ABO50276.1.
RefSeq	YP_001113101.1. NC_009253.1.
3D structure databases
ProteinModelPortal	A4J5C3.
ModBase	Search...
Protein-protein interaction databases
STRING	A4J5C3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4958627.
GenomeReviews	Gene locus Dred_1751 in contig CP000612_GR.
KEGG	drm:Dred_1751.
PATRIC	21729693. VBIDesRed82656_1920.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0620.
HOGENOM	HBG287495.
OMA	RNIWRAN.
PhylomeDB	A4J5C3.
ProtClustDB	PRK05222.
Enzyme and pathway databases
BioCyc	DRED349161:DRED_1751-MONOMER.
Family and domain databases
HAMAP	MF_00172. Meth_synth. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR006276. Cobalamin-indep_Met_synthase. IPR002629. Methionine_synth. [Graphical view]
KO	K00549.
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMs	TIGR01371. Met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_DESRM

Accession

Primary (citable) accession number: A4J5C3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	May 1, 2007
Last modified:	January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program