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Protein

Diaminopimelate epimerase

Gene

dapF

Organism
Desulfotomaculum reducens (strain MI-1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan.UniRule annotation

Catalytic activityi

LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate.UniRule annotation

Pathwayi: L-lysine biosynthesis via DAP pathway

This protein is involved in step 1 of the subpathway that synthesizes DL-2,6-diaminopimelate from LL-2,6-diaminopimelate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Diaminopimelate epimerase (dapF)
This subpathway is part of the pathway L-lysine biosynthesis via DAP pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes DL-2,6-diaminopimelate from LL-2,6-diaminopimelate, the pathway L-lysine biosynthesis via DAP pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei11SubstrateUniRule annotation1
Binding sitei64SubstrateUniRule annotation1
Active sitei73Proton donorUniRule annotation1
Binding sitei162SubstrateUniRule annotation1
Sitei164Could be important to modulate the pK values of the two catalytic cysteine residuesUniRule annotation1
Binding sitei195SubstrateUniRule annotation1
Sitei213Could be important to modulate the pK values of the two catalytic cysteine residuesUniRule annotation1
Active sitei222Proton acceptorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase
Biological processAmino-acid biosynthesis, Lysine biosynthesis

Enzyme and pathway databases

BioCyciDRED349161:G1G8H-1838-MONOMER
UniPathwayiUPA00034; UER00025

Names & Taxonomyi

Protein namesi
Recommended name:
Diaminopimelate epimeraseUniRule annotation (EC:5.1.1.7UniRule annotation)
Short name:
DAP epimeraseUniRule annotation
Alternative name(s):
PLP-independent amino acid racemaseUniRule annotation
Gene namesi
Name:dapFUniRule annotation
Ordered Locus Names:Dred_1694
OrganismiDesulfotomaculum reducens (strain MI-1)
Taxonomic identifieri349161 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesPeptococcaceaeDesulfotomaculum
Proteomesi
  • UP000001556 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000992321 – 284Diaminopimelate epimeraseAdd BLAST284

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi349161.Dred_1694

Structurei

3D structure databases

ProteinModelPortaliA4J568
SMRiA4J568
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni74 – 75Substrate bindingUniRule annotation2
Regioni213 – 214Substrate bindingUniRule annotation2
Regioni223 – 224Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the diaminopimelate epimerase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105E4Z Bacteria
COG0253 LUCA
HOGENOMiHOG000220466
KOiK01778
OMAiSMCGNGG
OrthoDBiPOG091H01QC

Family and domain databases

HAMAPiMF_00197 DAP_epimerase, 1 hit
InterProiView protein in InterPro
IPR018510 DAP_epimerase_AS
IPR001653 DAP_epimerase_DapF
PANTHERiPTHR31689 PTHR31689, 1 hit
PfamiView protein in Pfam
PF01678 DAP_epimerase, 2 hits
TIGRFAMsiTIGR00652 DapF, 1 hit
PROSITEiView protein in PROSITE
PS01326 DAP_EPIMERASE, 1 hit

Sequencei

Sequence statusi: Complete.

A4J568-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYFTKVHGLG NDFILVNAGT GQGFTDDCST LAKEVCDRKF GIGADGLVLL
60 70 80 90 100
YDSGVADVRM KIVNSDGSEA EMCGNAIRCV AKYLYDHGII KKDEIKVETL
110 120 130 140 150
AGIIVPQIIQ QEGSVKAVRV DMGEPRVERA EIPMIGPPGQ VIGEELAVNG
160 170 180 190 200
KVYLVTAVSM GNPHCIIFVP DLEDISLTQV GPQIEVHPAF PKKTNVEFVQ
210 220 230 240 250
VLGPNEVRMV VWERGAGPTM ACGTGACAVA VAGVLNGFTE RMVTVHLPGG
260 270 280
SLMIEWAENG RIYMTGPATE VFSGEYTVYN PSRR
Length:284
Mass (Da):30,534
Last modified:May 1, 2007 - v1
Checksum:i51EF0695062005F2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000612 Genomic DNA Translation: ABO50221.1
RefSeqiWP_011878036.1, NC_009253.1

Genome annotation databases

EnsemblBacteriaiABO50221; ABO50221; Dred_1694
KEGGidrm:Dred_1694

Similar proteinsi

Entry informationi

Entry nameiDAPF_DESRM
AccessioniPrimary (citable) accession number: A4J568
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: May 1, 2007
Last modified: March 28, 2018
This is version 71 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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