Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Queuine tRNA-ribosyltransferase

Gene

tgt

Organism
Desulfotomaculum reducens (strain MI-1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).UniRule annotation

Catalytic activityi

Guanine(34) in tRNA + queuine = queuosine(34) in tRNA + guanine.UniRule annotation
Guanine(34) in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7-carbaguanine(34) in tRNA + guanine.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Pathwayi: tRNA-queuosine biosynthesis

This protein is involved in the pathway tRNA-queuosine biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway tRNA-queuosine biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei93 – 931NucleophileUniRule annotation
Binding sitei94 – 941SubstrateUniRule annotation
Metal bindingi304 – 3041ZincUniRule annotation
Metal bindingi306 – 3061ZincUniRule annotation
Metal bindingi309 – 3091ZincUniRule annotation
Metal bindingi335 – 3351ZincUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Queuosine biosynthesis, tRNA processing

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciDRED349161:GHP6-1722-MONOMER.
UniPathwayiUPA00392.

Names & Taxonomyi

Protein namesi
Recommended name:
Queuine tRNA-ribosyltransferaseUniRule annotation (EC:2.4.2.29UniRule annotation)
Alternative name(s):
Guanine insertion enzymeUniRule annotation
tRNA-guanine transglycosylaseUniRule annotation
Gene namesi
Name:tgtUniRule annotation
Ordered Locus Names:Dred_1666
OrganismiDesulfotomaculum reducens (strain MI-1)
Taxonomic identifieri349161 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesPeptococcaceaeDesulfotomaculum
Proteomesi
  • UP000001556 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 370370Queuine tRNA-ribosyltransferasePRO_1000071574Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi349161.Dred_1666.

Structurei

3D structure databases

ProteinModelPortaliA4J541.
SMRiA4J541. Positions 6-368.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the queuine tRNA-ribosyltransferase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C6U. Bacteria.
COG0343. LUCA.
HOGENOMiHOG000223473.
KOiK00773.
OMAiMGVGKPD.
OrthoDBiEOG6SNDVG.

Family and domain databases

Gene3Di3.20.20.105. 1 hit.
HAMAPiMF_00168. Q_tRNA_Tgt.
InterProiIPR004803. Queuine_tRNA-ribosylTrfase.
IPR002616. tRNA_ribo_trans-like.
[Graphical view]
PfamiPF01702. TGT. 1 hit.
[Graphical view]
SUPFAMiSSF51713. SSF51713. 1 hit.
TIGRFAMsiTIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.

Sequencei

Sequence statusi: Complete.

A4J541-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPVKIKIEKK EKHTRARLGK LTTPHGEVET PIFMPVGTQA TVKTMTPEEV
60 70 80 90 100
KETGGRLVLS NTYHLYLRPG HDLVKEAGGL HKFMNWDGPI LTDSGGFQVF
110 120 130 140 150
SLGPLRTITE EGVEFRSHID GSKHFFTPEK VMEIEQALGA DIAMAFDECA
160 170 180 190 200
PYPCEKEYAV AALERTTRWA ERCKRVHKRE DQALFGIIQG GVFPDLRERS
210 220 230 240 250
AKELLAMDFP GYGIGGLSVG EPKELMYEVL DQLMPIMPED KPRYLMGVGS
260 270 280 290 300
PDCLIEGVVR GVDMFDCVLP TRIARNGTVF THNGKLTVRN AEYARDFRPM
310 320 330 340 350
DQQCDCYACR NYSRAYIRHL IKTDEILGIR LTTIHNLHFI QHLMQNIRQA
360 370
IREDRLLEYR EDFLKVFNAG
Length:370
Mass (Da):42,118
Last modified:May 1, 2007 - v1
Checksum:iD05F5DF8E0AFD696
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000612 Genomic DNA. Translation: ABO50194.1.
RefSeqiWP_011878009.1. NC_009253.1.

Genome annotation databases

EnsemblBacteriaiABO50194; ABO50194; Dred_1666.
KEGGidrm:Dred_1666.
PATRICi21729517. VBIDesRed82656_1832.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000612 Genomic DNA. Translation: ABO50194.1.
RefSeqiWP_011878009.1. NC_009253.1.

3D structure databases

ProteinModelPortaliA4J541.
SMRiA4J541. Positions 6-368.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi349161.Dred_1666.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABO50194; ABO50194; Dred_1666.
KEGGidrm:Dred_1666.
PATRICi21729517. VBIDesRed82656_1832.

Phylogenomic databases

eggNOGiENOG4105C6U. Bacteria.
COG0343. LUCA.
HOGENOMiHOG000223473.
KOiK00773.
OMAiMGVGKPD.
OrthoDBiEOG6SNDVG.

Enzyme and pathway databases

UniPathwayiUPA00392.
BioCyciDRED349161:GHP6-1722-MONOMER.

Family and domain databases

Gene3Di3.20.20.105. 1 hit.
HAMAPiMF_00168. Q_tRNA_Tgt.
InterProiIPR004803. Queuine_tRNA-ribosylTrfase.
IPR002616. tRNA_ribo_trans-like.
[Graphical view]
PfamiPF01702. TGT. 1 hit.
[Graphical view]
SUPFAMiSSF51713. SSF51713. 1 hit.
TIGRFAMsiTIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MI-1.

Entry informationi

Entry nameiTGT_DESRM
AccessioniPrimary (citable) accession number: A4J541
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: May 1, 2007
Last modified: December 9, 2015
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.