Reviewed, UniProtKB/Swiss-Prot A4J412 (SYN_DESRM)

Names and origin

Protein names

Recommended name:
    Asparaginyl-tRNA synthetase
    EC=6.1.1.22
Alternative name(s):
    Asparagine--tRNA ligase
      Short name=AsnRS

Gene names

Name:	asnS
Ordered Locus Names:	Dred_1281

Organism

Desulfotomaculum reducens (strain MI-1) [Complete proteome] [HAMAP]

Taxonomic identifier

349161 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Peptococcaceae › Desulfotomaculum

Protein attributes

Sequence length	429 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn). HAMAP MF_00534
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00534
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparaginyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP aspartyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP asparagine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP aspartate-tRNA ligase activity Inferred from electronic annotation. Source: InterPro nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 429	429	Asparaginyl-tRNA synthetase HAMAP MF_00534		PRO_1000072529

Sequences

	Sequence		Length	Mass (Da)
	A4J412-1 [UniParc]. Last modified May 1, 2007. Version 1. Checksum: 31A6A68D70DEA5F2	FASTA	429	49,675
	10 20 30 40 50 60 MQGTLIRHLG KHVDEEVSIQ GWLYNKRSSG KIQFLIVRDG TGLVQGVLVK KEAPEIFELA 70 80 90 100 110 120 KELTQESAIR LRGIVREEPR SMGGYELTVT GLEIINLAQE YPISHKEHGV DFLMDRRHLW 130 140 150 160 170 180 MRTPRQNAIL RIRAEIEQAA RDFFNQNDFT LVDSPIITPA ACEGTTTLFE LDYHGEKAYL 190 200 210 220 230 240 SQSGQLYNEA SAMAVGRMYC FGPTFRAEKS KTRRHLMEFW MIEAEAAFFD FEDNMKLQEE 250 260 270 280 290 300 MVYFIVQRVL ERRRQDLELL GRDISKLEAI KLPFPRLSYT EAVELLKSKG EAFEWGEDFG 310 320 330 340 350 360 APHETIISEN FESPVFIHRY PTEIKAFYMK PDPEDGRVVL GADLIAPEGY GEMIGGGQRI 370 380 390 400 410 420 DDLQLLEQRL EEHKLPKEAF EWYLDLRRYG SVPHSGFGLG LERTVAWICK LDHVRETIPY PRMLYRVYP

References

[1]

"Complete sequence of Desulfotomaculum reducens MI-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Han C., Tapia R., Schmutz J., Larimer F. , Land M., Hauser L., Kyrpides N., Kim E., Tebo B.M., Richardson P. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000612 Genomic DNA. Translation: ABO49815.1.
RefSeq	YP_001112640.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A4J412.
Genome annotation databases
GeneID	4958208.
GenomeReviews	Gene locus Dred_1281 in contig CP000612_GR.
KEGG	drm:Dred_1281.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	LQKKRHS.
Family and domain databases
HAMAP	MF_00534. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004522. Asn-tRNA-synth_IIb. IPR002312. Asp-tRNA-synth_IIb. IPR004365. NA_bd_OB_tRNA-helicase. [Graphical view]
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
TIGRFAMs	TIGR00457. asnS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYN_DESRM

Accession

Primary (citable) accession number: A4J412

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	May 1, 2007
Last modified:	November 3, 2009
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)