A4J3A0 (AROE_DESRM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Shikimate dehydrogenase
EC=1.1.1.25

Gene names

Name:	aroE
Ordered Locus Names:	Dred_1018

Organism

Desulfotomaculum reducens (strain MI-1) [Complete proteome] [HAMAP]

Taxonomic identifier

349161 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Peptococcaceae › Desulfotomaculum

Protein attributes

Sequence length	291 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Shikimate + NADP+ = 3-dehydroshikimate + NADPH. HAMAP MF_00222
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7. HAMAP MF_00222
Sequence similarities	Belongs to the shikimate dehydrogenase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro shikimate 3-dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 291	291	Shikimate dehydrogenase HAMAP MF_00222		PRO_0000325117
Regions
	Nucleotide binding	135 – 139	5	NADP By similarity
Sites
	Active site	74	1	Proton acceptor Potential

Sequences

	Sequence		Length	Mass (Da)
	A4J3A0 [UniParc]. Last modified May 1, 2007. Version 1. Checksum: 88DC6C834880F550	FASTA	291	31,947
	10 20 30 40 50 60 MGHCRINGKT QVCGLFGFPV EHSFSPAMHN AAFQQLDLNW TYVPFRVHPD NLKQAVTGIF 70 80 90 100 110 120 SLNMAGVNVT VPHKQRVMPF LDELEPAARI IGAVNTIVNN NGKLVGYNTD GKGFVRALTE 130 140 150 160 170 180 EARFNPLGKS AILIGAGGAA RAVAVQLALS GLRTIYITNR NQEKAEELAR DILESTDTSA 190 200 210 220 230 240 SMIPWGNNLL GKRMVEVDLV VQATPLGMSP EVDQVPEFPF QMLTPQHLVC DLIYNPEQTC 250 260 270 280 290 FLRRAKSRGS KTMNGLAMLL YQGVLAFELW TGFTAPVDVM RNVLHKQVAK G

References

[1]

"Complete sequence of Desulfotomaculum reducens MI-1." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Han C., Tapia R., Schmutz J., Larimer F. , Land M., Hauser L., Kyrpides N., Kim E., Tebo B.M., Richardson P. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MI-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000612 Genomic DNA. Translation: ABO49553.1.
RefSeq	YP_001112378.1. NC_009253.1.
3D structure databases
ProteinModelPortal	A4J3A0.
ModBase	Search...
Protein-protein interaction databases
STRING	A4J3A0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4956774.
GenomeReviews	Gene locus Dred_1018 in contig CP000612_GR.
KEGG	drm:Dred_1018.
PATRIC	21728085. VBIDesRed82656_1130.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0169.
HOGENOM	HBG553408.
OMA	SIYIDAT.
PhylomeDB	A4J3A0.
ProtClustDB	CLSK853475.
Enzyme and pathway databases
BioCyc	DRED349161:DRED_1018-MONOMER.
Family and domain databases
HAMAP	MF_00222. Shikimate_DH_AroE. [Tree]
InterPro	IPR016040. NAD(P)-bd_dom. IPR011342. Shikimate_DH. IPR013708. Shikimate_DH-bd_N. IPR022893. Shikimate_quinate_DH. IPR006151. Shikm_DH/Glu-tRNA_Rdtase. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00014.
Pfam	PF01488. Shikimate_DH. 1 hit. PF08501. Shikimate_dh_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00507. AroE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AROE_DESRM

Accession

Primary (citable) accession number: A4J3A0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	May 1, 2007
Last modified:	January 25, 2012
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program