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Protein

Shikimate dehydrogenase (NADP(+))

Gene

aroE

Organism
Desulfotomaculum reducens (strain MI-1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA).UniRule annotation

Catalytic activityi

Shikimate + NADP+ = 3-dehydroshikimate + NADPH.UniRule annotation

Pathwayi: chorismate biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase (aroQ)
  4. Shikimate dehydrogenase (NADP(+)) (aroE)
  5. Shikimate kinase (aroK), Shikimate kinase (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei70ShikimateUniRule annotation1
Active sitei74Proton acceptorUniRule annotation1
Binding sitei95ShikimateUniRule annotation1
Binding sitei110ShikimateUniRule annotation1
Binding sitei232NADP; via carbonyl oxygenUniRule annotation1
Binding sitei234ShikimateUniRule annotation1
Binding sitei255NADP; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi135 – 139NADPUniRule annotation5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processAmino-acid biosynthesis, Aromatic amino acid biosynthesis
LigandNADP

Enzyme and pathway databases

BioCyciDRED349161:G1G8H-1116-MONOMER
UniPathwayiUPA00053; UER00087

Names & Taxonomyi

Protein namesi
Recommended name:
Shikimate dehydrogenase (NADP(+))UniRule annotation (EC:1.1.1.25UniRule annotation)
Short name:
SDHUniRule annotation
Gene namesi
Name:aroEUniRule annotation
Ordered Locus Names:Dred_1018
OrganismiDesulfotomaculum reducens (strain MI-1)
Taxonomic identifieri349161 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesPeptococcaceaeDesulfotomaculum
Proteomesi
  • UP000001556 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003251171 – 291Shikimate dehydrogenase (NADP(+))Add BLAST291

Proteomic databases

PRIDEiA4J3A0

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi349161.Dred_1018

Structurei

3D structure databases

ProteinModelPortaliA4J3A0
SMRiA4J3A0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni23 – 25Shikimate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the shikimate dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105E2X Bacteria
COG0169 LUCA
HOGENOMiHOG000237875
KOiK00014
OMAiIPHKERA
OrthoDBiPOG091H02DY

Family and domain databases

HAMAPiMF_00222 Shikimate_DH_AroE, 1 hit
InterProiView protein in InterPro
IPR036291 NAD(P)-bd_dom_sf
IPR011342 Shikimate_DH
IPR013708 Shikimate_DH-bd_N
IPR022893 Shikimate_DH_fam
IPR006151 Shikm_DH/Glu-tRNA_Rdtase
PfamiView protein in Pfam
PF01488 Shikimate_DH, 1 hit
PF08501 Shikimate_dh_N, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR00507 aroE, 1 hit

Sequencei

Sequence statusi: Complete.

A4J3A0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGHCRINGKT QVCGLFGFPV EHSFSPAMHN AAFQQLDLNW TYVPFRVHPD
60 70 80 90 100
NLKQAVTGIF SLNMAGVNVT VPHKQRVMPF LDELEPAARI IGAVNTIVNN
110 120 130 140 150
NGKLVGYNTD GKGFVRALTE EARFNPLGKS AILIGAGGAA RAVAVQLALS
160 170 180 190 200
GLRTIYITNR NQEKAEELAR DILESTDTSA SMIPWGNNLL GKRMVEVDLV
210 220 230 240 250
VQATPLGMSP EVDQVPEFPF QMLTPQHLVC DLIYNPEQTC FLRRAKSRGS
260 270 280 290
KTMNGLAMLL YQGVLAFELW TGFTAPVDVM RNVLHKQVAK G
Length:291
Mass (Da):31,947
Last modified:May 1, 2007 - v1
Checksum:i88DC6C834880F550
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000612 Genomic DNA Translation: ABO49553.1
RefSeqiWP_011877380.1, NC_009253.1

Genome annotation databases

EnsemblBacteriaiABO49553; ABO49553; Dred_1018
KEGGidrm:Dred_1018

Similar proteinsi

Entry informationi

Entry nameiAROE_DESRM
AccessioniPrimary (citable) accession number: A4J3A0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: May 1, 2007
Last modified: May 23, 2018
This is version 77 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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