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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Desulfotomaculum reducens (strain MI-1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei559 – 5591Aminoacyl-adenylateUniRule annotation
Binding sitei603 – 6031ATPUniRule annotation
Metal bindingi898 – 8981ZincUniRule annotation
Metal bindingi901 – 9011ZincUniRule annotation
Metal bindingi918 – 9181ZincUniRule annotation
Metal bindingi921 – 9211ZincUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciDRED349161:GHP6-761-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Dred_0718
OrganismiDesulfotomaculum reducens (strain MI-1)
Taxonomic identifieri349161 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesPeptococcaceaeDesulfotomaculum
ProteomesiUP000001556 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 931931Isoleucine--tRNA ligasePRO_1000073708Add
BLAST

Proteomic databases

PRIDEiA4J2F4.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi349161.Dred_0718.

Structurei

3D structure databases

ProteinModelPortaliA4J2F4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionAdd
BLAST
Motifi600 – 6045"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4J2F4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYSKTLNLP QTQFPMRGNL PQREPETQKY WQEIDLYKKV QEKNKGKTKF
60 70 80 90 100
ILHDGPPYAN GHIHLGHTLN KVLKDIIVKY RSMSGYDAPY VPGWDTHGLP
110 120 130 140 150
IEQQAIKQLG INRHQVNPVE FRAKCKDYAL KWANTQSEEF QRLGVRGDWE
160 170 180 190 200
NPYYTLLPQY EATQIRVFGE MAKKGYIYKG LKPVYWCASC ETALAEAEVE
210 220 230 240 250
YADKTSPSIY VKFPVKDGKG VLPQDAAVVI WTTTPWTLPA NVAISVHPEF
260 270 280 290 300
EYVLAAVQNQ KIVVAKELLE SFKQAVGAEE AEILATYQGE QLERVVCQHP
310 320 330 340 350
FIDERESLVI LGEHVTLDAG TGAVHTAPGH GEEDFMVGKK YELPVLAPID
360 370 380 390 400
NRGRFTSEGG KFQGQFIMDA NKTIIEELKE RDALMGHVSI KHQYPHCWRC
410 420 430 440 450
KQPIFFRATE QWFASVDGFR QEALQAIRNV KWVPSWGEDR IYNMVEGRGD
460 470 480 490 500
WCVSRQRTWG VPIPIFYCND CGKEIITEET ISHIEKLFRE HGSDIWFAKE
510 520 530 540 550
ANELVPASLT CPHCGKGKDF RKETDTMDVW FDSGSSHLAV LNQPELWPEQ
560 570 580 590 600
QRPADLYLEG SDQHRGWFNS SLSTSVAVTG KPPYKTVLTH GFTVDEKGRK
610 620 630 640 650
MSKSLGNVVD PLKICSQMGA DILRLWVSSA DYRADLALSQ NILKQMTESY
660 670 680 690 700
RKIRNTARFL LGNLYDFDPV KDKVAYDKLP ELDRVALMEL HKLIKQVLAA
710 720 730 740 750
YENYEFHIVY HAVHNYCVVD LSAFYLDIIK DRLYTAVPGS LERRAAQTVL
760 770 780 790 800
YEALDALVRL LTPVLAFTTE EIYKYMPVVG DRLASVQMLD MPEVNVEYMD
810 820 830 840 850
VELEKKWDKI HEIRKEVLKA LEVARKNKVI GNALEAKVDL YVAGDVEEVL
860 870 880 890 900
KPMAAELTTL FIVSKVNLHG LAAAPADAVK AEELELALQV ATAEGGKCER
910 920 930
CWMYHEEVGN DAEHATLCPR CATVVKEHHT A
Length:931
Mass (Da):105,809
Last modified:May 1, 2007 - v1
Checksum:i1165C1F435AC996A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000612 Genomic DNA. Translation: ABO49257.1.
RefSeqiWP_011877093.1. NC_009253.1.

Genome annotation databases

EnsemblBacteriaiABO49257; ABO49257; Dred_0718.
KEGGidrm:Dred_0718.
PATRICi21727417. VBIDesRed82656_0796.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000612 Genomic DNA. Translation: ABO49257.1.
RefSeqiWP_011877093.1. NC_009253.1.

3D structure databases

ProteinModelPortaliA4J2F4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi349161.Dred_0718.

Proteomic databases

PRIDEiA4J2F4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABO49257; ABO49257; Dred_0718.
KEGGidrm:Dred_0718.
PATRICi21727417. VBIDesRed82656_0796.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciDRED349161:GHP6-761-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MI-1.

Entry informationi

Entry nameiSYI_DESRM
AccessioniPrimary (citable) accession number: A4J2F4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: May 1, 2007
Last modified: July 22, 2015
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.