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A4J2F4

- SYI_DESRM

UniProt

A4J2F4 - SYI_DESRM

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Protein

Isoleucine--tRNA ligase

Gene
ileS, Dred_0718
Organism
Desulfotomaculum reducens (strain MI-1)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei559 – 5591Aminoacyl-adenylate By similarity
Binding sitei603 – 6031ATP By similarity
Metal bindingi898 – 8981Zinc By similarity
Metal bindingi901 – 9011Zinc By similarity
Metal bindingi918 – 9181Zinc By similarity
Metal bindingi921 – 9211Zinc By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciDRED349161:GHP6-761-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:Dred_0718
OrganismiDesulfotomaculum reducens (strain MI-1)
Taxonomic identifieri349161 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesPeptococcaceaeDesulfotomaculum
ProteomesiUP000001556: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 931931Isoleucine--tRNA ligaseUniRule annotationPRO_1000073708Add
BLAST

Proteomic databases

PRIDEiA4J2F4.

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi349161.Dred_0718.

Structurei

3D structure databases

ProteinModelPortaliA4J2F4.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionUniRule annotationAdd
BLAST
Motifi600 – 6045"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiLGRRSCQ.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4J2F4-1 [UniParc]FASTAAdd to Basket

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MDYSKTLNLP QTQFPMRGNL PQREPETQKY WQEIDLYKKV QEKNKGKTKF    50
ILHDGPPYAN GHIHLGHTLN KVLKDIIVKY RSMSGYDAPY VPGWDTHGLP 100
IEQQAIKQLG INRHQVNPVE FRAKCKDYAL KWANTQSEEF QRLGVRGDWE 150
NPYYTLLPQY EATQIRVFGE MAKKGYIYKG LKPVYWCASC ETALAEAEVE 200
YADKTSPSIY VKFPVKDGKG VLPQDAAVVI WTTTPWTLPA NVAISVHPEF 250
EYVLAAVQNQ KIVVAKELLE SFKQAVGAEE AEILATYQGE QLERVVCQHP 300
FIDERESLVI LGEHVTLDAG TGAVHTAPGH GEEDFMVGKK YELPVLAPID 350
NRGRFTSEGG KFQGQFIMDA NKTIIEELKE RDALMGHVSI KHQYPHCWRC 400
KQPIFFRATE QWFASVDGFR QEALQAIRNV KWVPSWGEDR IYNMVEGRGD 450
WCVSRQRTWG VPIPIFYCND CGKEIITEET ISHIEKLFRE HGSDIWFAKE 500
ANELVPASLT CPHCGKGKDF RKETDTMDVW FDSGSSHLAV LNQPELWPEQ 550
QRPADLYLEG SDQHRGWFNS SLSTSVAVTG KPPYKTVLTH GFTVDEKGRK 600
MSKSLGNVVD PLKICSQMGA DILRLWVSSA DYRADLALSQ NILKQMTESY 650
RKIRNTARFL LGNLYDFDPV KDKVAYDKLP ELDRVALMEL HKLIKQVLAA 700
YENYEFHIVY HAVHNYCVVD LSAFYLDIIK DRLYTAVPGS LERRAAQTVL 750
YEALDALVRL LTPVLAFTTE EIYKYMPVVG DRLASVQMLD MPEVNVEYMD 800
VELEKKWDKI HEIRKEVLKA LEVARKNKVI GNALEAKVDL YVAGDVEEVL 850
KPMAAELTTL FIVSKVNLHG LAAAPADAVK AEELELALQV ATAEGGKCER 900
CWMYHEEVGN DAEHATLCPR CATVVKEHHT A 931
Length:931
Mass (Da):105,809
Last modified:May 1, 2007 - v1
Checksum:i1165C1F435AC996A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000612 Genomic DNA. Translation: ABO49257.1.
RefSeqiYP_001112082.1. NC_009253.1.

Genome annotation databases

EnsemblBacteriaiABO49257; ABO49257; Dred_0718.
GeneIDi4957249.
KEGGidrm:Dred_0718.
PATRICi21727417. VBIDesRed82656_0796.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000612 Genomic DNA. Translation: ABO49257.1 .
RefSeqi YP_001112082.1. NC_009253.1.

3D structure databases

ProteinModelPortali A4J2F4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 349161.Dred_0718.

Proteomic databases

PRIDEi A4J2F4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABO49257 ; ABO49257 ; Dred_0718 .
GeneIDi 4957249.
KEGGi drm:Dred_0718.
PATRICi 21727417. VBIDesRed82656_0796.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi LGRRSCQ.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci DRED349161:GHP6-761-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MI-1.

Entry informationi

Entry nameiSYI_DESRM
AccessioniPrimary (citable) accession number: A4J2F4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: May 1, 2007
Last modified: May 14, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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