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A4J2F4

- SYI_DESRM

UniProt

A4J2F4 - SYI_DESRM

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Desulfotomaculum reducens (strain MI-1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 57 (01 Oct 2014)
      Sequence version 1 (01 May 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei559 – 5591Aminoacyl-adenylateUniRule annotation
    Binding sitei603 – 6031ATPUniRule annotation
    Metal bindingi898 – 8981ZincUniRule annotation
    Metal bindingi901 – 9011ZincUniRule annotation
    Metal bindingi918 – 9181ZincUniRule annotation
    Metal bindingi921 – 9211ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciDRED349161:GHP6-761-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:Dred_0718
    OrganismiDesulfotomaculum reducens (strain MI-1)
    Taxonomic identifieri349161 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesPeptococcaceaeDesulfotomaculum
    ProteomesiUP000001556: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 931931Isoleucine--tRNA ligasePRO_1000073708Add
    BLAST

    Proteomic databases

    PRIDEiA4J2F4.

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi349161.Dred_0718.

    Structurei

    3D structure databases

    ProteinModelPortaliA4J2F4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi57 – 6711"HIGH" regionAdd
    BLAST
    Motifi600 – 6045"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiLGRRSCQ.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A4J2F4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDYSKTLNLP QTQFPMRGNL PQREPETQKY WQEIDLYKKV QEKNKGKTKF    50
    ILHDGPPYAN GHIHLGHTLN KVLKDIIVKY RSMSGYDAPY VPGWDTHGLP 100
    IEQQAIKQLG INRHQVNPVE FRAKCKDYAL KWANTQSEEF QRLGVRGDWE 150
    NPYYTLLPQY EATQIRVFGE MAKKGYIYKG LKPVYWCASC ETALAEAEVE 200
    YADKTSPSIY VKFPVKDGKG VLPQDAAVVI WTTTPWTLPA NVAISVHPEF 250
    EYVLAAVQNQ KIVVAKELLE SFKQAVGAEE AEILATYQGE QLERVVCQHP 300
    FIDERESLVI LGEHVTLDAG TGAVHTAPGH GEEDFMVGKK YELPVLAPID 350
    NRGRFTSEGG KFQGQFIMDA NKTIIEELKE RDALMGHVSI KHQYPHCWRC 400
    KQPIFFRATE QWFASVDGFR QEALQAIRNV KWVPSWGEDR IYNMVEGRGD 450
    WCVSRQRTWG VPIPIFYCND CGKEIITEET ISHIEKLFRE HGSDIWFAKE 500
    ANELVPASLT CPHCGKGKDF RKETDTMDVW FDSGSSHLAV LNQPELWPEQ 550
    QRPADLYLEG SDQHRGWFNS SLSTSVAVTG KPPYKTVLTH GFTVDEKGRK 600
    MSKSLGNVVD PLKICSQMGA DILRLWVSSA DYRADLALSQ NILKQMTESY 650
    RKIRNTARFL LGNLYDFDPV KDKVAYDKLP ELDRVALMEL HKLIKQVLAA 700
    YENYEFHIVY HAVHNYCVVD LSAFYLDIIK DRLYTAVPGS LERRAAQTVL 750
    YEALDALVRL LTPVLAFTTE EIYKYMPVVG DRLASVQMLD MPEVNVEYMD 800
    VELEKKWDKI HEIRKEVLKA LEVARKNKVI GNALEAKVDL YVAGDVEEVL 850
    KPMAAELTTL FIVSKVNLHG LAAAPADAVK AEELELALQV ATAEGGKCER 900
    CWMYHEEVGN DAEHATLCPR CATVVKEHHT A 931
    Length:931
    Mass (Da):105,809
    Last modified:May 1, 2007 - v1
    Checksum:i1165C1F435AC996A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000612 Genomic DNA. Translation: ABO49257.1.
    RefSeqiYP_001112082.1. NC_009253.1.

    Genome annotation databases

    EnsemblBacteriaiABO49257; ABO49257; Dred_0718.
    GeneIDi4957249.
    KEGGidrm:Dred_0718.
    PATRICi21727417. VBIDesRed82656_0796.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000612 Genomic DNA. Translation: ABO49257.1 .
    RefSeqi YP_001112082.1. NC_009253.1.

    3D structure databases

    ProteinModelPortali A4J2F4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 349161.Dred_0718.

    Proteomic databases

    PRIDEi A4J2F4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABO49257 ; ABO49257 ; Dred_0718 .
    GeneIDi 4957249.
    KEGGi drm:Dred_0718.
    PATRICi 21727417. VBIDesRed82656_0796.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi LGRRSCQ.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci DRED349161:GHP6-761-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: MI-1.

    Entry informationi

    Entry nameiSYI_DESRM
    AccessioniPrimary (citable) accession number: A4J2F4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 26, 2008
    Last sequence update: May 1, 2007
    Last modified: October 1, 2014
    This is version 57 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3