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Protein

Lipoyl synthase

Gene

lipA

Organism
Desulfotomaculum reducens (strain MI-1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Catalytic activityi

Protein N6-(octanoyl)lysine + an [Fe-S] cluster scaffold protein carrying a [4Fe-4S]2+ cluster + 2 S-adenosyl-L-methionine + 2 oxidized [2Fe-2S] ferredoxin + 6 H+ = protein N6-(dihydrolipoyl)lysine + an [Fe-S] cluster scaffold protein + 2 sulfide + 4 Fe3+ + 2 L-methionine + 2 5'-deoxyadenosine + 2 reduced [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi: protein lipoylation via endogenous pathway

This protein is involved in step 2 of the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein].UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Octanoyltransferase (lipB)
  2. Lipoyl synthase (lipA)
This subpathway is part of the pathway protein lipoylation via endogenous pathway, which is itself part of Protein modification.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein], the pathway protein lipoylation via endogenous pathway and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi35Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi40Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi46Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi61Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi65Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi68Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionTransferase
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciDRED349161:G1G8H-670-MONOMER
UniPathwayiUPA00538; UER00593

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthaseUniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lip-synUniRule annotation
Short name:
LSUniRule annotation
Lipoate synthaseUniRule annotation
Lipoic acid synthaseUniRule annotation
Sulfur insertion protein LipAUniRule annotation
Gene namesi
Name:lipAUniRule annotation
Ordered Locus Names:Dred_0610
OrganismiDesulfotomaculum reducens (strain MI-1)
Taxonomic identifieri349161 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesPeptococcaceaeDesulfotomaculum
Proteomesi

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003252451 – 283Lipoyl synthaseAdd BLAST283

Proteomic databases

PRIDEiA4J247

Interactioni

Protein-protein interaction databases

STRINGi349161.Dred_0610

Structurei

3D structure databases

ProteinModelPortaliA4J247
SMRiA4J247
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C0G Bacteria
COG0320 LUCA
HOGENOMiHOG000235998
KOiK03644
OMAiPYCDIDF
OrthoDBiPOG091H069D

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00206 Lipoyl_synth, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR006638 Elp3/MiaB/NifB
IPR031691 LIAS_N
IPR003698 Lipoyl_synth
IPR007197 rSAM
PfamiView protein in Pfam
PF16881 LIAS_N, 1 hit
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF005963 Lipoyl_synth, 1 hit
SFLDiSFLDG01058 lipoyl_synthase_like, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00510 lipA, 1 hit

Sequencei

Sequence statusi: Complete.

A4J247-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKRKPDWLK IKLQGAEKSH EVKDMLKRLS LHTVCEEANC PNLIECFGRK
60 70 80 90 100
TATFMILGSV CTRNCTFCNV TKGLTQAVDA EEPSNVAQAV KELGLKHVVI
110 120 130 140 150
TSVTRDDLPD GGAGHFAKVI EKLRPTEVIV EVLIPDFQGD REALDTVIRA
160 170 180 190 200
KPHILNHNIE TVPRLYATVR PKASYARSLE LLKNSKELDP GIFTKSGIMV
210 220 230 240 250
GLGEQEEEVI AVLQDLRAVD CDLLTIGQYL APSAKHHPVI EYIHPELFKK
260 270 280
YKDVAYEMGF KYVASDPLVR SSYHAADVSH IIG
Length:283
Mass (Da):31,512
Last modified:May 1, 2007 - v1
Checksum:iD44AC17BFCB2E614
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000612 Genomic DNA Translation: ABO49150.1
RefSeqiWP_011876987.1, NC_009253.1

Genome annotation databases

EnsemblBacteriaiABO49150; ABO49150; Dred_0610
KEGGidrm:Dred_0610

Entry informationi

Entry nameiLIPA_DESRM
AccessioniPrimary (citable) accession number: A4J247
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: May 1, 2007
Last modified: May 23, 2018
This is version 72 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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