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A4J233 (THIM_DESRM) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hydroxyethylthiazole kinase

EC=2.7.1.50
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinase
Short name=TH kinase
Short name=Thz kinase
Gene names
Name:thiM
Ordered Locus Names:Dred_0594
OrganismDesulfotomaculum reducens (strain MI-1) [Complete proteome] [HAMAP]
Taxonomic identifier349161 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesPeptococcaceaeDesulfotomaculum

Protein attributes

Sequence length266 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole. HAMAP-Rule MF_00228

Cofactor

Magnesium By similarity. HAMAP-Rule MF_00228

Pathway

Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole: step 1/1. HAMAP-Rule MF_00228

Sequence similarities

Belongs to the Thz kinase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 266266Hydroxyethylthiazole kinase HAMAP-Rule MF_00228
PRO_0000336554

Sites

Binding site461Substrate; via amide nitrogen By similarity
Binding site1221ATP By similarity
Binding site1671ATP By similarity
Binding site1941Substrate; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
A4J233 [UniParc].

Last modified May 1, 2007. Version 1.
Checksum: DF42AF8049094371

FASTA26628,568
        10         20         30         40         50         60 
MQEVQRLWDI RQEVRIKKPL VCNITNTVVT NFTANVLLAV GASPLMSEGW QEADDLAKIV 

        70         80         90        100        110        120 
DVLVLNMGTL HPRQVEYFIK AGKSANQEQK PVVFDPVGIG ATSYRNAVAA EILDKIKLDL 

       130        140        150        160        170        180 
IRGNHGEINF LAGITGQTKG VDSLSNQISV KHLAEFANTT RTLVVSTGEV DYLSDGAKTF 

       190        200        210        220        230        240 
TNKTGHAYLQ LVTGTGCALS SLAGAFMSVT EDKCLGVLSA LAFYGAAAQK AALISQGPGT 

       250        260 
FAGNFLDALY GLEFDEFKKI LEAPKI 

« Hide

References

[1]"Complete sequence of Desulfotomaculum reducens MI-1."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Han C., Tapia R., Schmutz J., Larimer F. expand/collapse author list , Land M., Hauser L., Kyrpides N., Kim E., Tebo B.M., Richardson P.
Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: MI-1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000612 Genomic DNA. Translation: ABO49136.1.
RefSeqYP_001111961.1. NC_009253.1.

3D structure databases

ProteinModelPortalA4J233.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING349161.Dred_0594.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABO49136; ABO49136; Dred_0594.
GeneID4958211.
KEGGdrm:Dred_0594.
PATRIC21727135. VBIDesRed82656_0655.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2145.
HOGENOMHOG000114352.
KOK00878.
OMASPVMAHA.
OrthoDBEOG628F8M.
ProtClustDBCLSK717776.

Enzyme and pathway databases

BioCycDRED349161:GHP6-638-MONOMER.
UniPathwayUPA00060; UER00139.

Family and domain databases

HAMAPMF_00228. Thz_kinase.
InterProIPR000417. Hyethyz_kinase.
[Graphical view]
PfamPF02110. HK. 1 hit.
[Graphical view]
PIRSFPIRSF000513. Thz_kinase. 1 hit.
PRINTSPR01099. HYETHTZKNASE.
TIGRFAMsTIGR00694. thiM. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTHIM_DESRM
AccessionPrimary (citable) accession number: A4J233
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 1, 2007
Last modified: February 19, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways