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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Desulfotomaculum reducens (strain MI-1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei253ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciDRED349161:G1G8H-209-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:Dred_0191
OrganismiDesulfotomaculum reducens (strain MI-1)
Taxonomic identifieri349161 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesPeptococcaceaeDesulfotomaculum
Proteomesi
  • UP000001556 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003676651 – 482Glutamate--tRNA ligaseAdd BLAST482

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi349161.Dred_0191

Structurei

3D structure databases

ProteinModelPortaliA4J0Y7
SMRiA4J0Y7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi9 – 19"HIGH" regionUniRule annotationAdd BLAST11
Motifi250 – 254"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C20 Bacteria
COG0008 LUCA
HOGENOMiHOG000252720
KOiK09698
OMAiAYYAFDT
OrthoDBiPOG091H021W

Family and domain databases

CDDicd00808 GluRS_core, 1 hit
Gene3Di1.10.10.350, 1 hit
3.40.50.620, 1 hit
HAMAPiMF_00022 Glu_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR008925 aa-tRNA-synth_I_codon-bd
IPR020751 aa-tRNA-synth_I_codon-bd_sub2
IPR001412 aa-tRNA-synth_I_CS
IPR004527 Glu-tRNA-ligase_bac/mito
IPR000924 Glu/Gln-tRNA-synth
IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom
IPR033910 GluRS_core
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00749 tRNA-synt_1c, 1 hit
PRINTSiPR00987 TRNASYNTHGLU
SUPFAMiSSF48163 SSF48163, 1 hit
TIGRFAMsiTIGR00464 gltX_bact, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

A4J0Y7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIRVRFAPS PTGPLHIGGA RSALFNWLYA RHHGGQFLVR IEDTDMERSS
60 70 80 90 100
RESEENILNA LRWLGIDWDE GIEVGGPNGP YRQTERLDIY RQLAKELVDA
110 120 130 140 150
GHAYYCYCSE EELAVEREAL MEKGELPRYL GRCRNLCPED KAKFEAEGKK
160 170 180 190 200
PVLRFRVPEN QTITINDHVR GQVEFESNGI GDFIIMKSDN IPTYNFAVVV
210 220 230 240 250
DDHDMGITHV VRAEEHLSNT PRQILIYEAL GWQKPEFAHI SLILGKDRSK
260 270 280 290 300
MSKRHGATSI EQYHNLGYLP EALVNFLALL GWSPGGEEEI FTLKEIKEQF
310 320 330 340 350
TLDRVAKNPA VFDIDKLNWL NGHYIRQASV ERLTKLAVPY LQGAGYLKEE
360 370 380 390 400
ISTEKMAWLE QVVAIARNYI SYMQEITQHV DVFFRDNVEI TEEDAKQVQG
410 420 430 440 450
WEQMPVVMKA AHELFSAANE LTEESVKAII KAIGKQTGLK GKFIFQPLRV
460 470 480
GITGQTHGPE LHQIIPVIGK ERTLARLEAA LK
Length:482
Mass (Da):54,866
Last modified:May 1, 2007 - v1
Checksum:i94B493153CE04B92
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000612 Genomic DNA Translation: ABO48740.1
RefSeqiWP_011876581.1, NC_009253.1

Genome annotation databases

EnsemblBacteriaiABO48740; ABO48740; Dred_0191
KEGGidrm:Dred_0191

Similar proteinsi

Entry informationi

Entry nameiSYE_DESRM
AccessioniPrimary (citable) accession number: A4J0Y7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: May 1, 2007
Last modified: May 23, 2018
This is version 70 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

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