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A4IYP1

- SYI_FRATW

UniProt

A4IYP1 - SYI_FRATW

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Francisella tularensis subsp. tularensis (strain WY96-3418)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 58 (01 Oct 2014)
      Sequence version 1 (01 May 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei558 – 5581Aminoacyl-adenylateUniRule annotation
    Binding sitei602 – 6021ATPUniRule annotation
    Metal bindingi897 – 8971ZincUniRule annotation
    Metal bindingi900 – 9001ZincUniRule annotation
    Metal bindingi917 – 9171ZincUniRule annotation
    Metal bindingi920 – 9201ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciFTUL418136:GHXJ-1181-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:FTW_1264
    OrganismiFrancisella tularensis subsp. tularensis (strain WY96-3418)
    Taxonomic identifieri418136 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaThiotrichalesFrancisellaceaeFrancisella
    ProteomesiUP000002285: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 935935Isoleucine--tRNA ligasePRO_1000022070Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi418136.FTW_1264.

    Structurei

    3D structure databases

    ProteinModelPortaliA4IYP1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi58 – 6811"HIGH" regionAdd
    BLAST
    Motifi599 – 6035"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A4IYP1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDYKDTLNL PKTSFSMKGN LANKEPMILN KWEKQGIYKK IREHFAGREK    50
    FVLHDGPPYA NGSIHVGHAV NKILKDIIIK SKTLSGYDAP FTPTWDCHGL 100
    PIELQVEKKH GKAGQSISED DFRKECRKYA KKQVEIQKKD FKRLGVLGDW 150
    EQPYLTINFD YEANMIRTLA KIIENGHLSK GFKPVHWCTD CGSALAEAEV 200
    EYADKVSPAI DVKFKIKDKD KLAQAFGLDS LNHDAFAIIW TTTPWTLPAN 250
    QAIAVNNQLN YSLIKIEDFY IILAENLVEQ TLKRYAIENA QIIATTTGNK 300
    LTGIIAEHPF YSRHVPILHG DHVTDDSGTG LVHTAPTHGV DDFTLGKEHN 350
    LSMEIFVKGN GCYSENTKLF AGEFIFKAND RIIELLGEKK RLMNSDKIKH 400
    SYPHCWRHKT PLMFRATPQW FISMEKQGLR DKALQAIKET SWAPSWGQAR 450
    IEGMVKDRPD WCISRQRTWG VPLPLFIHKE TEELHPNTIE ILHKVAEKIE 500
    KDGIEAWFNA DDCEFITETA QYKSVKDTLD VWFDSGSSSM CILDLDKRLS 550
    YPADLYLEGS DQHRGWFQTS LLVAMSAKGS QPYKEVFTHG FVVDEHGRKM 600
    SKSLGNVTSP QDIYNTLGAD ILRLWTASTD YKSEMAVSDQ ILKRTADTYR 650
    RLRNTARFLL SNLDGFNPVT DIIEFDKLVK LDQWAIAKTK EFQDKIIEAY 700
    DKYQTHTVAQ LIHHFCSIEM GSFYLDIIKD RQYTAKTDGH PRKSAQTAIY 750
    HIVHALVRWM APILSFTANE IWDATPKTTD LPIQLCEWYT GLKSFDQDAE 800
    LDLEYWAKIQ EIRSEVNRVL EIKRNEDVIK ASLEAEITIY ADKYNYKLLE 850
    KLGNELRFLL ISSKADLKVI EESTSSSIAA NIPGLLIEIT KIEEPKCERC 900
    WHRSSTVGDN PQYKDICSRC VENITTEAGE SREFA 935
    Length:935
    Mass (Da):106,934
    Last modified:May 1, 2007 - v1
    Checksum:i3129B312D304A0BB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000608 Genomic DNA. Translation: ABO47042.1.
    RefSeqiYP_001122163.1. NC_009257.1.

    Genome annotation databases

    EnsemblBacteriaiABO47042; ABO47042; FTW_1264.
    GeneIDi4960029.
    KEGGiftw:FTW_1264.
    PATRICi17969982. VBIFraTul58433_1302.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000608 Genomic DNA. Translation: ABO47042.1 .
    RefSeqi YP_001122163.1. NC_009257.1.

    3D structure databases

    ProteinModelPortali A4IYP1.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 418136.FTW_1264.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABO47042 ; ABO47042 ; FTW_1264 .
    GeneIDi 4960029.
    KEGGi ftw:FTW_1264.
    PATRICi 17969982. VBIFraTul58433_1302.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci FTUL418136:GHXJ-1181-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: WY96-3418.

    Entry informationi

    Entry nameiSYI_FRATW
    AccessioniPrimary (citable) accession number: A4IYP1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: May 1, 2007
    Last modified: October 1, 2014
    This is version 58 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3