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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Francisella tularensis subsp. tularensis (strain WY96-3418)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei578 – 5781ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciFTUL418136:GHXJ-849-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:FTW_0897
OrganismiFrancisella tularensis subsp. tularensis (strain WY96-3418)
Taxonomic identifieri418136 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaThiotrichalesFrancisellaceaeFrancisella
ProteomesiUP000002285 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 813813Leucine--tRNA ligasePRO_1000009345Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi418136.FTW_0897.

Structurei

3D structure databases

ProteinModelPortaliA4IXU1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi41 – 5111"HIGH" regionAdd
BLAST
Motifi575 – 5795"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4IXU1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNEYNFSDIE KSTQEYWRKN DTFKTIEDNT KEKFYCLSML PYPSGTLHMG
60 70 80 90 100
HVRNYTIGDV IARYQKMQGK NVLHPMGWDA FGLPAENAAI KHKKSPYEWT
110 120 130 140 150
KSNIAYMRSQ FDSLGFSFDW SREIATCDED YYKWEQWFFI QLYKKGLAYR
160 170 180 190 200
KNSVVNWDPV DQTVLANEQV VDGRGWRSGA LVEKKEIPQW FLKITDYADE
210 220 230 240 250
LLQDINKLDN WPEAVKTMQI NWIGKSKGLT VKFKVKDSNQ EIEVFTTRPD
260 270 280 290 300
TLMGVNYLGI APEHPLALKE AKSNSQLAAF IEECKKTSTM EADLATQEKK
310 320 330 340 350
GFKTSIKVIH PISAETIDVW VANFVLMGYG SGAVMSVPAH DQRDWEFAQK
360 370 380 390 400
YNIPLKQVIE SNDNKLKIDL EKQAFTEKGI LINSGEFDGL NFKNAYQAIK
410 420 430 440 450
KYLTEQNKGY ETTNFRIHDW GISRQRYWGC PIPMIHCDDC GAVPEKEENL
460 470 480 490 500
PVRLPTDVAL TEAGSPLKDI PEFINVACPE CGKPAKRETD TFDTFFESSW
510 520 530 540 550
YYARYTCPTA NQMLDQEANY WLPVDKYIGG IEHAIMHLLY ARFFHKLMRD
560 570 580 590 600
QGLVKSDEPF KNLLTQGMVL KDGAKMSKSK GNIVDPQELI DKYGADTVRL
610 620 630 640 650
FSMFAAPPEQ SLEWSETGVE GANKFLRKVF NYAELNKVIF AKNITLESQK
660 670 680 690 700
LTKEDKKARF EIHSNLKQAI FDFDKSQFNT VVSACMKILN TLNNYDNLSE
710 720 730 740 750
SVKVEGFSIL LRILAPFTPH LCHYLWQQLN LGEDILHTSF PIVDNNALEK
760 770 780 790 800
DEFLLVVQIN GKLKAKLELD ASLSSNQVEE VVLADEHVKS FIDNKQVVKV
810
IYVPQKLINI VIK
Length:813
Mass (Da):93,413
Last modified:May 1, 2007 - v1
Checksum:iA8B64B2A86EFDA47
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000608 Genomic DNA. Translation: ABO46742.1.
RefSeqiYP_001121863.1. NC_009257.1.

Genome annotation databases

EnsemblBacteriaiABO46742; ABO46742; FTW_0897.
KEGGiftw:FTW_0897.
PATRICi17969219. VBIFraTul58433_0923.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000608 Genomic DNA. Translation: ABO46742.1.
RefSeqiYP_001121863.1. NC_009257.1.

3D structure databases

ProteinModelPortaliA4IXU1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi418136.FTW_0897.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABO46742; ABO46742; FTW_0897.
KEGGiftw:FTW_0897.
PATRICi17969219. VBIFraTul58433_0923.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciFTUL418136:GHXJ-849-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: WY96-3418.

Entry informationi

Entry nameiSYL_FRATW
AccessioniPrimary (citable) accession number: A4IXU1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 1, 2007
Last modified: April 1, 2015
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.