A4IWV0 (GLO2_FRATW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hydroxyacylglutathione hydrolase
EC=3.1.2.6

Alternative name(s):
Glyoxalase II
Short name=Glx II

Gene names

Name:	gloB
Ordered Locus Names:	FTW_0483

Organism

Francisella tularensis subsp. tularensis (strain WY96-3418) [Complete proteome] [HAMAP]

Taxonomic identifier

418136 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Thiotrichales › Francisellaceae › Francisella

Protein attributes

Sequence length	252 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid By similarity. HAMAP MF_01374
Catalytic activity	S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate. HAMAP MF_01374
Cofactor	Binds 2 zinc ions per subunit By similarity. HAMAP MF_01374
Pathway	Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2. HAMAP MF_01374
Subunit structure	Monomer By similarity. HAMAP MF_01374
Sequence similarities	Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	hydroxyacylglutathione hydrolase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 252	252	Hydroxyacylglutathione hydrolase HAMAP MF_01374		PRO_0000309646
Sites
	Metal binding	54	1	Zinc 1 By similarity
	Metal binding	56	1	Zinc 1 By similarity
	Metal binding	58	1	Zinc 2 By similarity
	Metal binding	59	1	Zinc 2 By similarity
	Metal binding	111	1	Zinc 1 By similarity
	Metal binding	130	1	Zinc 1 By similarity
	Metal binding	130	1	Zinc 2 By similarity
	Metal binding	170	1	Zinc 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4IWV0-1 [UniParc]. Last modified May 1, 2007. Version 1. Checksum: 98FF547F7B4E0ED7	FASTA	252	28,849
	10 20 30 40 50 60 MQIKRWFLNN SLRNYQYLLY DKSHAIVIDP LKSDIFAEFI AKNKLQLEAI LITHKHGDHI 70 80 90 100 110 120 AGVKKLLAIY LNAKVYAYTG NDLFKPDIYV KDGSFINLGF TSFRVMYIPG HIDDHVCFLF 130 140 150 160 170 180 EQERALFCGD TLFNAGVGGV QAESADINQL YDSLVKITKL DGDIKPYPAH DYWLGNLDFA 190 200 210 220 230 240 LSILADDSYF NYYRNQVAEL AAEDKPIVNL AEEAKLNIFI RAMSDKALLK ALPDYSLGRE 250 MFVKLRQLKN NF

References

[1]

"Complete genomic characterization of a pathogenic A.II strain of Francisella tularensis subspecies tularensis." Beckstrom-Sternberg S.M., Auerbach R.K., Godbole S., Pearson J.V., Beckstrom-Sternberg J.S., Deng Z., Munk C., Kubota K., Zhou Y., Bruce D., Noronha J., Scheuermann R.H., Wang A., Wei X., Wang J., Hao J., Wagner D.M., Brettin T.S. , Brown N., Gilna P., Keim P.S. PLoS ONE 2:E947-E947(2007) [PubMed: 17895988] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000608 Genomic DNA. Translation: ABO46402.1.
RefSeq	YP_001121522.1.
3D structure databases
ProteinModelPortal	A4IWV0.
SMR	A4IWV0. Positions 13-252.
ModBase	Search...
Protein-protein interaction databases
STRING	A4IWV0.
Genome annotation databases
GeneID	4959885.
GenomeReviews	Gene locus FTW_0483 in contig CP000608_GR.
KEGG	ftw:FTW_0483.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0491.
HOGENOM	HBG753931.
OMA	NTHEAVW.
ProtClustDB	CLSK935013.
Enzyme and pathway databases
BioCyc	FTUL418136:FTW_0483-MONOMER.
Family and domain databases
HAMAP	MF_01374. Glyoxalase_2. [Tree]
InterPro	IPR001279. Blactmase-like. IPR017782. Hydroxyacylglutathione_Hdrlase. [Graphical view]
Pfam	PF00753. Lactamase_B. 1 hit. [Graphical view]
SMART	SM00849. Lactamase_B. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLO2_FRATW

Accession

Primary (citable) accession number: A4IWV0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	May 1, 2007
Last modified:	August 10, 2010
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)