A4IT59 (T23O_GEOTN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tryptophan 2,3-dioxygenase
Short name=TDO
EC=1.13.11.11

Alternative name(s):
Tryptamin 2,3-dioxygenase
Tryptophan oxygenase
Short name=TO
Short name=TRPO
Tryptophan pyrrolase
Tryptophanase

Gene names

Name:	kynA
Ordered Locus Names:	GTNG_3168

Organism

Geobacillus thermodenitrificans (strain NG80-2) [Complete proteome] [HAMAP]

Taxonomic identifier

420246 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Geobacillus

Protein attributes

Sequence length	280 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the oxidative cleavage of the L-tryptophan (L-Trp) pyrrole ring By similarity.
Catalytic activity	L-tryptophan + O2 = N-formyl-L-kynurenine.
Cofactor	Binds 2 heme groups per tetramer By similarity.
Pathway	Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the tryptophan 2,3-dioxygenase family.

Ontologies

Keywords
Biological process	Tryptophan catabolism
Ligand	Heme Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein homotetramerization Inferred from sequence or structural similarity. Source: UniProtKB tryptophan catabolic process to kynurenine Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	heme binding Inferred from sequence or structural similarity. Source: UniProtKB tryptophan 2,3-dioxygenase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 280	280	Tryptophan 2,3-dioxygenase		PRO_0000360116
Regions
	Region	24 – 28	5	Substrate binding By similarity
	Region	49 – 53	5	Substrate binding By similarity
Sites
	Metal binding	238	1	Iron (heme axial ligand) By similarity
	Binding site	111	1	Substrate By similarity
	Binding site	115	1	Substrate By similarity
	Binding site	122	1	Heme By similarity
	Binding site	252	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4IT59 [UniParc]. Last modified May 1, 2007. Version 1. Checksum: AC1D4A3B0DBF06E5	FASTA	280	32,823
	10 20 30 40 50 60 MQNKQNQFEL EKGIHTDFQE EMSYAGYLQL DKILSSQQRL SNHHDEMLFI IIHQTSELWM 70 80 90 100 110 120 KLILHEMEAA IQCILDDDLE PSFKMFARIS RIQQQLIQSW SVLSTLTPSE YMEFRECLGH 130 140 150 160 170 180 ASGFQSYQNR LIEFALGQKN AHVLPVFQHD PEIYEKMTRA LHSPSVYDAA IKALAVRGLP 190 200 210 220 230 240 IDEDVLHRDY SQTYQPNASV EEAWLTVYRN VDRYWDLYEL AEKLVDIGSQ QQTWRYMHMN 250 260 270 280 TVERIIGYKK GTGGSSGVSY LKKSLDRSFF PELWTLRTKL

References

[1]

"Genome and proteome of long-chain alkane degrading Geobacillus thermodenitrificans NG80-2 isolated from a deep-subsurface oil reservoir." Feng L., Wang W., Cheng J., Ren Y., Zhao G., Gao C., Tang Y., Liu X., Han W., Peng X., Liu R., Wang L. Proc. Natl. Acad. Sci. U.S.A. 104:5602-5607(2007) [PubMed: 17372208] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NG80-2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000557 Genomic DNA. Translation: ABO68513.1.
RefSeq	YP_001127258.1. NC_009328.1.
3D structure databases
ProteinModelPortal	A4IT59.
SMR	A4IT59. Positions 21-280.
ModBase	Search...
Protein-protein interaction databases
STRING	A4IT59.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4968094.
GenomeReviews	Gene locus GTNG_3168 in contig CP000557_GR.
KEGG	gtn:GTNG_3168.
NMPDR	fig|420246.5.peg.3031.
PATRIC	21982975. VBIGeoThe136879_3330.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3483.
HOGENOM	HBG647485.
OMA	KLLVDQV.
ProtClustDB	CLSK2787298.
Enzyme and pathway databases
BioCyc	GTHE420246:GTNG_3168-MONOMER.
Family and domain databases
InterPro	IPR017485. Trp_2-3-dOase_bac. IPR004981. Trp_2_3_dOase. [Graphical view]
KO	K00453.
PANTHER	PTHR10138. Trp_2_3_dOase. 1 hit.
Pfam	PF03301. Trp_dioxygenase. 1 hit. [Graphical view]
TIGRFAMs	TIGR03036. Trp_2_3_diox. 1 hit.
ProtoNet	Search...

Entry information

Entry name

T23O_GEOTN

Accession

Primary (citable) accession number: A4IT59

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	May 1, 2007
Last modified:	December 14, 2011
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program