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A4IT50

- RIBF_GEOTN

UniProt

A4IT50 - RIBF_GEOTN

Protein

Putative riboflavin biosynthesis protein RibF

Gene

ribF

Organism
Geobacillus thermodenitrificans (strain NG80-2)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 40 (01 Oct 2014)
      Sequence version 1 (01 May 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + riboflavin = ADP + FMN.1 Publication
    ATP + FMN = diphosphate + FAD.1 Publication

    Kineticsi

      Vmax=270.3 µmol/min/mg enzyme toward FMN1 Publication

      Vmax=170.09 µmol/min/mg enzyme toward riboflavin1 Publication

      Pathwayi

      GO - Molecular functioni

      1. ATP binding Source: UniProtKB-KW
      2. FMN adenylyltransferase activity Source: UniProtKB-EC
      3. riboflavin kinase activity Source: UniProtKB-EC

      GO - Biological processi

      1. FAD biosynthetic process Source: UniProtKB-UniPathway
      2. FMN biosynthetic process Source: UniProtKB-UniPathway
      3. riboflavin biosynthetic process Source: InterPro

      Keywords - Molecular functioni

      Kinase, Nucleotidyltransferase, Transferase

      Keywords - Ligandi

      ATP-binding, FAD, Flavoprotein, FMN, Nucleotide-binding

      Enzyme and pathway databases

      BioCyciGTHE420246:GIXT-3255-MONOMER.
      UniPathwayiUPA00276; UER00406.
      UPA00277; UER00407.

      Names & Taxonomyi

      Protein namesi
      Recommended name:
      Putative riboflavin biosynthesis protein RibF
      Including the following 2 domains:
      Riboflavin kinase1 Publication (EC:2.7.1.261 Publication)
      Alternative name(s):
      FlavokinaseBy similarity
      FMN adenylyltransferase1 Publication (EC:2.7.7.21 Publication)
      Alternative name(s):
      FAD pyrophosphorylaseBy similarity
      FAD synthaseBy similarity
      Gene namesi
      Name:ribF
      Ordered Locus Names:GTNG_3159
      OrganismiGeobacillus thermodenitrificans (strain NG80-2)
      Taxonomic identifieri420246 [NCBI]
      Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus
      ProteomesiUP000001578: Chromosome

      PTM / Processingi

      Molecule processing

      Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
      Chaini1 – 179179Putative riboflavin biosynthesis protein RibFPRO_0000421234Add
      BLAST

      Interactioni

      Protein-protein interaction databases

      STRINGi420246.GTNG_3159.

      Structurei

      3D structure databases

      ProteinModelPortaliA4IT50.
      ModBaseiSearch...
      MobiDBiSearch...

      Family & Domainsi

      Sequence similaritiesi

      Belongs to the RibF family.Sequence Analysis

      Phylogenomic databases

      eggNOGiCOG0196.
      HOGENOMiHOG000088656.
      OMAiITKSASC.
      OrthoDBiEOG6QP0ZV.

      Family and domain databases

      Gene3Di3.40.50.620. 1 hit.
      InterProiIPR015864. FAD_synthase.
      IPR023468. Riboflavin_kinase.
      IPR014729. Rossmann-like_a/b/a_fold.
      [Graphical view]
      PANTHERiPTHR22749. PTHR22749. 1 hit.
      PfamiPF06574. FAD_syn. 1 hit.
      [Graphical view]

      Sequencei

      Sequence statusi: Complete.

      A4IT50-1 [UniParc]FASTAAdd to Basket

      « Hide

      MKVHEANQGL TLPGSVVAIG AFDGVHQGHQ AVLRQAVERS RQLGVESVAY    50
      TIDPPPRCRF QGSRMLTTLQ EKLDRFAVLG LNHAVVAHFD ERYAARRVDA 100
      FIRELTALNP REVIVGQDFR FGRNREGDVA LLRRHFPVRI VQTVCCADGQ 150
      RISSTRIREL IERGEWEQST VLLGWPLSS 179
      Length:179
      Mass (Da):20,167
      Last modified:May 1, 2007 - v1
      Checksum:i78CC00E8A22FCE33
      GO

      Sequence databases

      Select the link destinations:
      EMBL
      GenBank
      DDBJ
      Links Updated
      CP000557 Genomic DNA. Translation: ABO68504.1.
      RefSeqiWP_011888288.1. NC_009328.1.
      YP_001127249.1. NC_009328.1.

      Genome annotation databases

      EnsemblBacteriaiABO68504; ABO68504; GTNG_3159.
      GeneIDi4968085.
      KEGGigtn:GTNG_3159.
      PATRICi21982961. VBIGeoThe136879_3323.

      Cross-referencesi

      Sequence databases

      Select the link destinations:
      EMBL
      GenBank
      DDBJ
      Links Updated
      CP000557 Genomic DNA. Translation: ABO68504.1 .
      RefSeqi WP_011888288.1. NC_009328.1.
      YP_001127249.1. NC_009328.1.

      3D structure databases

      ProteinModelPortali A4IT50.
      ModBasei Search...
      MobiDBi Search...

      Protein-protein interaction databases

      STRINGi 420246.GTNG_3159.

      Protocols and materials databases

      Structural Biology Knowledgebase Search...

      Genome annotation databases

      EnsemblBacteriai ABO68504 ; ABO68504 ; GTNG_3159 .
      GeneIDi 4968085.
      KEGGi gtn:GTNG_3159.
      PATRICi 21982961. VBIGeoThe136879_3323.

      Phylogenomic databases

      eggNOGi COG0196.
      HOGENOMi HOG000088656.
      OMAi ITKSASC.
      OrthoDBi EOG6QP0ZV.

      Enzyme and pathway databases

      UniPathwayi UPA00276 ; UER00406 .
      UPA00277 ; UER00407 .
      BioCyci GTHE420246:GIXT-3255-MONOMER.

      Family and domain databases

      Gene3Di 3.40.50.620. 1 hit.
      InterProi IPR015864. FAD_synthase.
      IPR023468. Riboflavin_kinase.
      IPR014729. Rossmann-like_a/b/a_fold.
      [Graphical view ]
      PANTHERi PTHR22749. PTHR22749. 1 hit.
      Pfami PF06574. FAD_syn. 1 hit.
      [Graphical view ]
      ProtoNeti Search...

      Publicationsi

      1. "Genome and proteome of long-chain alkane degrading Geobacillus thermodenitrificans NG80-2 isolated from a deep-subsurface oil reservoir."
        Feng L., Wang W., Cheng J., Ren Y., Zhao G., Gao C., Tang Y., Liu X., Han W., Peng X., Liu R., Wang L.
        Proc. Natl. Acad. Sci. U.S.A. 104:5602-5607(2007) [PubMed] [Europe PMC] [Abstract]
        Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
        Strain: NG80-2.
      2. "Characterization of the anthranilate degradation pathway in Geobacillus thermodenitrificans NG80-2."
        Liu X., Dong Y., Li X., Ren Y., Li Y., Wang W., Wang L., Feng L.
        Microbiology 156:589-595(2010) [PubMed] [Europe PMC] [Abstract]
        Cited for: CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
        Strain: NG80-21 Publication.

      Entry informationi

      Entry nameiRIBF_GEOTN
      AccessioniPrimary (citable) accession number: A4IT50
      Entry historyi
      Integrated into UniProtKB/Swiss-Prot: February 6, 2013
      Last sequence update: May 1, 2007
      Last modified: October 1, 2014
      This is version 40 of the entry and version 1 of the sequence. [Complete history]
      Entry statusiReviewed (UniProtKB/Swiss-Prot)
      Annotation programProkaryotic Protein Annotation Program

      Miscellaneousi

      Keywords - Technical termi

      Complete proteome, Multifunctional enzyme

      Documents

      1. PATHWAY comments
        Index of metabolic and biosynthesis pathways
      2. SIMILARITY comments
        Index of protein domains and families

      External Data

      Dasty 3