A4IT50 (RIBF_GEOTN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative riboflavin biosynthesis protein RibF

Including the following 2 domains:

1. Riboflavin kinase
   EC=2.7.1.26
   Alternative name(s):
   Flavokinase
2. FMN adenylyltransferase
   EC=2.7.7.2
   Alternative name(s):
   FAD pyrophosphorylase
   FAD synthase

Gene names

Name:	ribF
Ordered Locus Names:	GTNG_3159

Organism

Geobacillus thermodenitrificans (strain NG80-2) [Complete proteome] [HAMAP]

Taxonomic identifier

420246 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Geobacillus › Bacillus thermodenitrificans

Protein attributes

Sequence length	179 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + riboflavin = ADP + FMN. Ref.2 ATP + FMN = diphosphate + FAD. Ref.2
Pathway	Cofactor biosynthesis; FAD biosynthesis; FAD from FMN: step 1/1. UniProtKB Q59263 Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (ATP route): step 1/1. UniProtKB Q59263
Sequence similarities	Belongs to the RibF family.
Biophysicochemical properties	Kinetic parameters: Vmax=270.3 µmol/min/mg enzyme toward FMN Ref.2 Vmax=170.09 µmol/min/mg enzyme toward riboflavin Ref.2

Ontologies

Keywords
Ligand	ATP-binding FAD FMN Nucleotide-binding
Molecular function	Kinase Nucleotidyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological_process	FAD biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway FMN biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway riboflavin biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW FMN adenylyltransferase activity Inferred from electronic annotation. Source: EC riboflavin kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 179	179	Putative riboflavin biosynthesis protein RibF		PRO_0000421234

Sequences

	Sequence		Length	Mass (Da)
	A4IT50 [UniParc]. Last modified May 1, 2007. Version 1. Checksum: 78CC00E8A22FCE33	FASTA	179	20,167
	10 20 30 40 50 60 MKVHEANQGL TLPGSVVAIG AFDGVHQGHQ AVLRQAVERS RQLGVESVAY TIDPPPRCRF 70 80 90 100 110 120 QGSRMLTTLQ EKLDRFAVLG LNHAVVAHFD ERYAARRVDA FIRELTALNP REVIVGQDFR 130 140 150 160 170 FGRNREGDVA LLRRHFPVRI VQTVCCADGQ RISSTRIREL IERGEWEQST VLLGWPLSS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome and proteome of long-chain alkane degrading Geobacillus thermodenitrificans NG80-2 isolated from a deep-subsurface oil reservoir." Feng L., Wang W., Cheng J., Ren Y., Zhao G., Gao C., Tang Y., Liu X., Han W., Peng X., Liu R., Wang L. Proc. Natl. Acad. Sci. U.S.A. 104:5602-5607(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NG80-2.
[2]	"Characterization of the anthranilate degradation pathway in Geobacillus thermodenitrificans NG80-2." Liu X., Dong Y., Li X., Ren Y., Li Y., Wang W., Wang L., Feng L. Microbiology 156:589-595(2010) [PubMed] [Europe PMC] [Abstract] Cited for: CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES. Strain: NG80-2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000557 Genomic DNA. Translation: ABO68504.1.
RefSeq	YP_001127249.1. NC_009328.1.
3D structure databases
ProteinModelPortal	A4IT50.
ModBase	Search...
Protein-protein interaction databases
STRING	420246.GTNG_3159.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABO68504; ABO68504; GTNG_3159.
GeneID	4968085.
KEGG	gtn:GTNG_3159.
PATRIC	21982961. VBIGeoThe136879_3323.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0196.
HOGENOM	HOG000088656.
OMA	KVHEANQ.
ProtClustDB	CLSK704129.
Enzyme and pathway databases
BioCyc	GTHE420246:GIXT-3255-MONOMER.
UniPathway	UPA00276; UER00406. UPA00277; UER00407.
Family and domain databases
Gene3D	3.40.50.620. 1 hit.
InterPro	IPR015864. FAD_synthase. IPR023468. Riboflavin_kinase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
PANTHER	PTHR22749. PTHR22749. 1 hit.
Pfam	PF06574. FAD_syn. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIBF_GEOTN

Accession

Primary (citable) accession number: A4IT50

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 6, 2013
Last sequence update:	May 1, 2007
Last modified:	May 1, 2013
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program