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A4IT50 (RIBF_GEOTN) Reviewed, UniProtKB/Swiss-Prot

Last modified January 22, 2014. Version 37. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Putative riboflavin biosynthesis protein RibF

Including the following 2 domains:

  1. Riboflavin kinase
    EC=2.7.1.26
    Alternative name(s):
    Flavokinase
  2. FMN adenylyltransferase
    EC=2.7.7.2
    Alternative name(s):
    FAD pyrophosphorylase
    FAD synthase
Gene names
Name:ribF
Ordered Locus Names:GTNG_3159
OrganismGeobacillus thermodenitrificans (strain NG80-2) [Complete proteome] [HAMAP]
Taxonomic identifier420246 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

Protein attributes

Sequence length179 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + riboflavin = ADP + FMN. Ref.2

ATP + FMN = diphosphate + FAD. Ref.2

Pathway

Cofactor biosynthesis; FAD biosynthesis; FAD from FMN: step 1/1. UniProtKB Q59263

Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (ATP route): step 1/1. UniProtKB Q59263

Sequence similarities

Belongs to the RibF family.

Biophysicochemical properties

Kinetic parameters:

Vmax=270.3 µmol/min/mg enzyme toward FMN Ref.2

Vmax=170.09 µmol/min/mg enzyme toward riboflavin Ref.2

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 179179Putative riboflavin biosynthesis protein RibF
PRO_0000421234

Sequences

Sequence LengthMass (Da)Tools
A4IT50 [UniParc].

Last modified May 1, 2007. Version 1.
Checksum: 78CC00E8A22FCE33

FASTA17920,167
        10         20         30         40         50         60 
MKVHEANQGL TLPGSVVAIG AFDGVHQGHQ AVLRQAVERS RQLGVESVAY TIDPPPRCRF 

        70         80         90        100        110        120 
QGSRMLTTLQ EKLDRFAVLG LNHAVVAHFD ERYAARRVDA FIRELTALNP REVIVGQDFR 

       130        140        150        160        170 
FGRNREGDVA LLRRHFPVRI VQTVCCADGQ RISSTRIREL IERGEWEQST VLLGWPLSS 

« Hide

References

« Hide 'large scale' references
[1]"Genome and proteome of long-chain alkane degrading Geobacillus thermodenitrificans NG80-2 isolated from a deep-subsurface oil reservoir."
Feng L., Wang W., Cheng J., Ren Y., Zhao G., Gao C., Tang Y., Liu X., Han W., Peng X., Liu R., Wang L.
Proc. Natl. Acad. Sci. U.S.A. 104:5602-5607(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NG80-2.
[2]"Characterization of the anthranilate degradation pathway in Geobacillus thermodenitrificans NG80-2."
Liu X., Dong Y., Li X., Ren Y., Li Y., Wang W., Wang L., Feng L.
Microbiology 156:589-595(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
Strain: NG80-2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000557 Genomic DNA. Translation: ABO68504.1.
RefSeqYP_001127249.1. NC_009328.1.

3D structure databases

ProteinModelPortalA4IT50.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING420246.GTNG_3159.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABO68504; ABO68504; GTNG_3159.
GeneID4968085.
KEGGgtn:GTNG_3159.
PATRIC21982961. VBIGeoThe136879_3323.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0196.
HOGENOMHOG000088656.
OMAITARFRA.
OrthoDBEOG6QP0ZV.
ProtClustDBCLSK704129.

Enzyme and pathway databases

BioCycGTHE420246:GIXT-3255-MONOMER.
UniPathwayUPA00276; UER00406.
UPA00277; UER00407.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
InterProIPR015864. FAD_synthase.
IPR023468. Riboflavin_kinase.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR22749. PTHR22749. 1 hit.
PfamPF06574. FAD_syn. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRIBF_GEOTN
AccessionPrimary (citable) accession number: A4IT50
Entry history
Integrated into UniProtKB/Swiss-Prot: February 6, 2013
Last sequence update: May 1, 2007
Last modified: January 22, 2014
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways