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A4ISG5

- LIPA_GEOTN

UniProt

A4ISG5 - LIPA_GEOTN

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Protein
Lipoyl synthase
Gene
lipA, GTNG_2924
Organism
Geobacillus thermodenitrificans (strain NG80-2)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein].

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi40 – 401Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi45 – 451Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi51 – 511Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi67 – 671Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi71 – 711Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi74 – 741Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciGTHE420246:GIXT-3019-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase (EC:2.8.1.8)
Alternative name(s):
Lip-syn
Short name:
LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA
Gene namesi
Name:lipA
Ordered Locus Names:GTNG_2924
OrganismiGeobacillus thermodenitrificans (strain NG80-2)
Taxonomic identifieri420246 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus
ProteomesiUP000001578: Chromosome

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 298298Lipoyl synthaseUniRule annotation
PRO_1000012226Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi420246.GTNG_2924.

Structurei

3D structure databases

ProteinModelPortaliA4ISG5.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235998.
KOiK03644.
OMAiHPHIPTK.
OrthoDBiEOG6038ZS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

A4ISG5-1 [UniParc]FASTAAdd to Basket

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MATKEEHVRK PDWLKIKLNT NEHYTGLKKL MRENRLHTVC EEAKCPNIHE    50
CWAVRRTATF MILGSVCTRA CRFCAVKTGL PTELDWQEPE RVAESVRIMN 100
LKHVVVTAVA RDDLKDGGAA VFAETVRAIR RKNPFTTIEV LPSDMGGVYE 150
NLKTLMDARP DILNHNIETV RRLTPRVRAR ATYERSLEFL RRAKELQPDI 200
PTKSSIMIGL GETKEEIIEA MDDLRANHVD ILTIGQYLQP TKKHLKVVKY 250
YHPDEFQELK EIALSKGFSH CEAGPLVRSS YHADEQVNEA AKARQLKA 298
Length:298
Mass (Da):34,096
Last modified:May 1, 2007 - v1
Checksum:i03BFAAB0BF5C6877
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000557 Genomic DNA. Translation: ABO68269.1.
RefSeqiYP_001127014.1. NC_009328.1.

Genome annotation databases

EnsemblBacteriaiABO68269; ABO68269; GTNG_2924.
GeneIDi4967850.
KEGGigtn:GTNG_2924.
PATRICi21982471. VBIGeoThe136879_3079.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000557 Genomic DNA. Translation: ABO68269.1 .
RefSeqi YP_001127014.1. NC_009328.1.

3D structure databases

ProteinModelPortali A4ISG5.
ModBasei Search...

Protein-protein interaction databases

STRINGi 420246.GTNG_2924.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABO68269 ; ABO68269 ; GTNG_2924 .
GeneIDi 4967850.
KEGGi gtn:GTNG_2924.
PATRICi 21982471. VBIGeoThe136879_3079.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235998.
KOi K03644.
OMAi HPHIPTK.
OrthoDBi EOG6038ZS.

Enzyme and pathway databases

BioCyci GTHE420246:GIXT-3019-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Genome and proteome of long-chain alkane degrading Geobacillus thermodenitrificans NG80-2 isolated from a deep-subsurface oil reservoir."
    Feng L., Wang W., Cheng J., Ren Y., Zhao G., Gao C., Tang Y., Liu X., Han W., Peng X., Liu R., Wang L.
    Proc. Natl. Acad. Sci. U.S.A. 104:5602-5607(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NG80-2.

Entry informationi

Entry nameiLIPA_GEOTN
AccessioniPrimary (citable) accession number: A4ISG5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 1, 2007
Last modified: May 14, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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