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Protein

Phosphoenolpyruvate carboxykinase [ATP]

Gene

pckA

Organism
Geobacillus thermodenitrificans (strain NG80-2)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the gluconeogenesis. Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP) through direct phosphoryl transfer between the nucleoside triphosphate and OAA.UniRule annotation

Catalytic activityi

ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei56 – 561SubstrateUniRule annotation
Binding sitei192 – 1921SubstrateUniRule annotation
Metal bindingi198 – 1981ManganeseUniRule annotation
Binding sitei198 – 1981ATPUniRule annotation
Binding sitei198 – 1981SubstrateUniRule annotation
Metal bindingi217 – 2171Manganese; via tele nitrogenUniRule annotation
Binding sitei217 – 2171ATPUniRule annotation
Metal bindingi254 – 2541ManganeseUniRule annotation
Binding sitei282 – 2821ATPUniRule annotation
Binding sitei319 – 3191ATPUniRule annotation
Binding sitei319 – 3191SubstrateUniRule annotation
Binding sitei444 – 4441ATPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi233 – 2419ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

ATP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciGTHE420246:GIXT-2828-MONOMER.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [ATP]UniRule annotation (EC:4.1.1.49UniRule annotation)
Short name:
PCKUniRule annotation
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckAUniRule annotation
Ordered Locus Names:GTNG_2751
OrganismiGeobacillus thermodenitrificans (strain NG80-2)
Taxonomic identifieri420246 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus
Proteomesi
  • UP000001578 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 528528Phosphoenolpyruvate carboxykinase [ATP]PRO_1000026326Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi420246.GTNG_2751.

Structurei

3D structure databases

ProteinModelPortaliA4IRZ2.
SMRiA4IRZ2. Positions 16-526.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [ATP] family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DJ1. Bacteria.
COG1866. LUCA.
HOGENOMiHOG000271471.
KOiK01610.
OMAiRFIVKEP.
OrthoDBiPOG091H03CD.

Family and domain databases

CDDicd00484. PEPCK_ATP. 1 hit.
Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00453. PEPCK_ATP. 1 hit.
InterProiIPR001272. PEP_carboxykinase_ATP.
IPR013035. PEP_carboxykinase_C.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PfamiPF01293. PEPCK_ATP. 1 hit.
[Graphical view]
PIRSFiPIRSF006294. PEP_crbxkin. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
TIGRFAMsiTIGR00224. pckA. 1 hit.

Sequencei

Sequence statusi: Complete.

A4IRZ2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGIAKMTNKL SLLLQKPYVH HQLSVAELVE KVLHRNEGRL THTGAVAVTT
60 70 80 90 100
GKYTGRSPKD KYIVEEPSTK QNIDWGTVNQ PMAPETFQKL YDKVLDYLMK
110 120 130 140 150
KDELFVFKGF AGADPKARLP IQVVNEFAWH NLFVHQLFIR PSAEELADHE
160 170 180 190 200
PQFTVICAPN FKADPAVDGT RSEAFIIISF EQRTVLIGGT EYAGEMKKSI
210 220 230 240 250
FSVMNYLLPE QGILPMHCSA NVGQEGDVAL FFGLSGTGKT TLSTDPKRRL
260 270 280 290 300
IGDDEHGWSS RGIFNIEGGC YAKCINLSRE KEPQIFDAIG FGAVLENVIL
310 320 330 340 350
HDATRVPDYD DGTLTENTRA AYPLQAIKNI VDPSVAGHPS TIVFLTADAF
360 370 380 390 400
GVLPPISKLT REQAMYHFLS GYTSKLAGTE RGVTEPEATF STCFGAPFLP
410 420 430 440 450
RPAVEYAEML GQKIAEHNVR VFLVNTGWTG GPYGVGSRMK LAYTRAMVQA
460 470 480 490 500
AVEGELDNVE TVQDPIFGLA IPAHVPGVPD DVLRPQNTWA DKQAYEQKAK
510 520
ELAEKFRANF QKFAHIDPSI EKLGGPLV
Length:528
Mass (Da):58,259
Last modified:May 1, 2007 - v1
Checksum:iE314FF36301B178D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000557 Genomic DNA. Translation: ABO68096.1.
RefSeqiWP_008880953.1. NC_009328.1.

Genome annotation databases

EnsemblBacteriaiABO68096; ABO68096; GTNG_2751.
KEGGigtn:GTNG_2751.
PATRICi21982075. VBIGeoThe136879_2899.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000557 Genomic DNA. Translation: ABO68096.1.
RefSeqiWP_008880953.1. NC_009328.1.

3D structure databases

ProteinModelPortaliA4IRZ2.
SMRiA4IRZ2. Positions 16-526.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi420246.GTNG_2751.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABO68096; ABO68096; GTNG_2751.
KEGGigtn:GTNG_2751.
PATRICi21982075. VBIGeoThe136879_2899.

Phylogenomic databases

eggNOGiENOG4105DJ1. Bacteria.
COG1866. LUCA.
HOGENOMiHOG000271471.
KOiK01610.
OMAiRFIVKEP.
OrthoDBiPOG091H03CD.

Enzyme and pathway databases

UniPathwayiUPA00138.
BioCyciGTHE420246:GIXT-2828-MONOMER.

Family and domain databases

CDDicd00484. PEPCK_ATP. 1 hit.
Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00453. PEPCK_ATP. 1 hit.
InterProiIPR001272. PEP_carboxykinase_ATP.
IPR013035. PEP_carboxykinase_C.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PfamiPF01293. PEPCK_ATP. 1 hit.
[Graphical view]
PIRSFiPIRSF006294. PEP_crbxkin. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
TIGRFAMsiTIGR00224. pckA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPCKA_GEOTN
AccessioniPrimary (citable) accession number: A4IRZ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 1, 2007
Last modified: September 7, 2016
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.