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Protein

Queuine tRNA-ribosyltransferase

Gene

tgt

Organism
Geobacillus thermodenitrificans (strain NG80-2)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).UniRule annotation

Catalytic activityi

Guanine(34) in tRNA + queuine = queuosine(34) in tRNA + guanine.UniRule annotation
Guanine(34) in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7-carbaguanine(34) in tRNA + guanine.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Pathwayi: tRNA-queuosine biosynthesis

This protein is involved in the pathway tRNA-queuosine biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway tRNA-queuosine biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei95 – 951NucleophileUniRule annotation
Binding sitei96 – 961SubstrateUniRule annotation
Metal bindingi307 – 3071ZincUniRule annotation
Metal bindingi309 – 3091ZincUniRule annotation
Metal bindingi312 – 3121ZincUniRule annotation
Metal bindingi338 – 3381ZincUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Queuosine biosynthesis, tRNA processing

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciGTHE420246:GIXT-2595-MONOMER.
UniPathwayiUPA00392.

Names & Taxonomyi

Protein namesi
Recommended name:
Queuine tRNA-ribosyltransferaseUniRule annotation (EC:2.4.2.29UniRule annotation)
Alternative name(s):
Guanine insertion enzymeUniRule annotation
tRNA-guanine transglycosylaseUniRule annotation
Gene namesi
Name:tgtUniRule annotation
Ordered Locus Names:GTNG_2518
OrganismiGeobacillus thermodenitrificans (strain NG80-2)
Taxonomic identifieri420246 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus
Proteomesi
  • UP000001578 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 380380Queuine tRNA-ribosyltransferasePRO_1000016796Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi420246.GTNG_2518.

Structurei

3D structure databases

ProteinModelPortaliA4IRA9.
SMRiA4IRA9. Positions 4-370.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the queuine tRNA-ribosyltransferase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C6U. Bacteria.
COG0343. LUCA.
HOGENOMiHOG000223473.
KOiK00773.
OMAiMGVGKPD.
OrthoDBiEOG6SNDVG.

Family and domain databases

Gene3Di3.20.20.105. 1 hit.
HAMAPiMF_00168. Q_tRNA_Tgt.
InterProiIPR004803. Queuine_tRNA-ribosylTrfase.
IPR002616. tRNA_ribo_trans-like.
[Graphical view]
PfamiPF01702. TGT. 1 hit.
[Graphical view]
SUPFAMiSSF51713. SSF51713. 1 hit.
TIGRFAMsiTIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.

Sequencei

Sequence statusi: Complete.

A4IRA9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTPIRFELI KTCRQTGARL GILHTPHGSF ETPMFMPVGT LATVKTLSPE
60 70 80 90 100
ELKEMGAGVI LSNTYHLWLR PGHDIVAEAG GLHAFMNWDR GILTDSGGFQ
110 120 130 140 150
VFSLSEFRRI EEEGVYFRNH LNGDKLFLSP EKATEIQNAL GADIIMAFDE
160 170 180 190 200
CPPYPATYDY MKRSVERTSR WAERCLKAHR RSNEQGLFGI VQGGEYEDLR
210 220 230 240 250
RQSARDLVSL DFPGYAVGGL SVGEPKDVMN RVLEFTTPLL PTDKPRYLMG
260 270 280 290 300
VGSPDSLIDG AIRGIDMFDC VLPTRIGRNG TVMTSEGRVV IKNAQYARDF
310 320 330 340 350
TPLDPNCDCY TCRNYTRAYI RHLIKCDETF GIRLTSYHNV YFLIKLMEQV
360 370 380
RQAIREDRLA DFREEFFERY GFNKPNAKNF
Length:380
Mass (Da):43,273
Last modified:May 1, 2007 - v1
Checksum:i6B240930ECF3707E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000557 Genomic DNA. Translation: ABO67863.1.
RefSeqiWP_008881045.1. NC_009328.1.

Genome annotation databases

EnsemblBacteriaiABO67863; ABO67863; GTNG_2518.
KEGGigtn:GTNG_2518.
PATRICi21981581. VBIGeoThe136879_2652.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000557 Genomic DNA. Translation: ABO67863.1.
RefSeqiWP_008881045.1. NC_009328.1.

3D structure databases

ProteinModelPortaliA4IRA9.
SMRiA4IRA9. Positions 4-370.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi420246.GTNG_2518.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABO67863; ABO67863; GTNG_2518.
KEGGigtn:GTNG_2518.
PATRICi21981581. VBIGeoThe136879_2652.

Phylogenomic databases

eggNOGiENOG4105C6U. Bacteria.
COG0343. LUCA.
HOGENOMiHOG000223473.
KOiK00773.
OMAiMGVGKPD.
OrthoDBiEOG6SNDVG.

Enzyme and pathway databases

UniPathwayiUPA00392.
BioCyciGTHE420246:GIXT-2595-MONOMER.

Family and domain databases

Gene3Di3.20.20.105. 1 hit.
HAMAPiMF_00168. Q_tRNA_Tgt.
InterProiIPR004803. Queuine_tRNA-ribosylTrfase.
IPR002616. tRNA_ribo_trans-like.
[Graphical view]
PfamiPF01702. TGT. 1 hit.
[Graphical view]
SUPFAMiSSF51713. SSF51713. 1 hit.
TIGRFAMsiTIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Genome and proteome of long-chain alkane degrading Geobacillus thermodenitrificans NG80-2 isolated from a deep-subsurface oil reservoir."
    Feng L., Wang W., Cheng J., Ren Y., Zhao G., Gao C., Tang Y., Liu X., Han W., Peng X., Liu R., Wang L.
    Proc. Natl. Acad. Sci. U.S.A. 104:5602-5607(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NG80-2.

Entry informationi

Entry nameiTGT_GEOTN
AccessioniPrimary (citable) accession number: A4IRA9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 1, 2007
Last modified: December 9, 2015
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.