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A4IR30

- DNAJ_GEOTN

UniProt

A4IR30 - DNAJ_GEOTN

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Protein

Chaperone protein DnaJ

Gene

dnaJ

Organism
Geobacillus thermodenitrificans (strain NG80-2)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins.UniRule annotation

Cofactori

Binds 2 zinc ions per monomer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi149 – 1491Zinc 1UniRule annotation
Metal bindingi152 – 1521Zinc 1UniRule annotation
Metal bindingi166 – 1661Zinc 2UniRule annotation
Metal bindingi169 – 1691Zinc 2UniRule annotation
Metal bindingi192 – 1921Zinc 2UniRule annotation
Metal bindingi195 – 1951Zinc 2UniRule annotation
Metal bindingi206 – 2061Zinc 1UniRule annotation
Metal bindingi209 – 2091Zinc 1UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri136 – 21883CR-typeUniRule annotationAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: InterPro
  2. nucleic acid binding Source: InterPro
  3. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA replication Source: UniProtKB-KW
  2. protein folding Source: InterPro
  3. response to heat Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

DNA replication, Stress response

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciGTHE420246:GIXT-2516-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Chaperone protein DnaJUniRule annotation
Gene namesi
Name:dnaJUniRule annotation
Ordered Locus Names:GTNG_2439
OrganismiGeobacillus thermodenitrificans (strain NG80-2)
Taxonomic identifieri420246 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus
ProteomesiUP000001578: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 381381Chaperone protein DnaJPRO_1000085199Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi420246.GTNG_2439.

Structurei

3D structure databases

ProteinModelPortaliA4IR30.
SMRiA4IR30. Positions 2-71.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 6965JUniRule annotationAdd
BLAST
Repeati149 – 1568CXXCXGXG motif
Repeati166 – 1738CXXCXGXG motif
Repeati192 – 1998CXXCXGXG motif
Repeati206 – 2138CXXCXGXG motif

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi76 – 11944Gly-richAdd
BLAST

Domaini

The J domain is necessary and sufficient to stimulate DnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, DnaK-independent chaperone activity of DnaJ. Zinc center 2 is essential for interaction with DnaK and for DnaJ activity.UniRule annotation

Sequence similaritiesi

Belongs to the DnaJ family.UniRule annotation
Contains 1 CR-type zinc finger.UniRule annotation
Contains 1 J domain.UniRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri136 – 21883CR-typeUniRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG0484.
HOGENOMiHOG000226717.
KOiK03686.
OMAiGHTDPNQ.
OrthoDBiEOG6BPDKP.

Family and domain databases

Gene3Di1.10.287.110. 1 hit.
2.10.230.10. 1 hit.
HAMAPiMF_01152. DnaJ.
InterProiIPR012724. DnaJ.
IPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
IPR001305. HSP_DnaJ_Cys-rich_dom.
IPR001878. Znf_CCHC.
[Graphical view]
PfamiPF01556. CTDII. 2 hits.
PF00226. DnaJ. 1 hit.
PF00684. DnaJ_CXXCXGXG. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
SM00343. ZnF_C2HC. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 3 hits.
SSF57938. SSF57938. 1 hit.
TIGRFAMsiTIGR02349. DnaJ_bact. 1 hit.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51188. ZF_CR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4IR30-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAKRDYYEVL GVSKNATKDE IKKAYRKLSK QYHPDINKAP DAAEKFKEIK
60 70 80 90 100
EAYEVLSDDE KRARYDRFGH ADPNEAFGGG FQGGGFDFGG FSGFGGFEDI
110 120 130 140 150
FETFFGGGSR RRASGPRKGA DLEYMMTLTF EEAAFGKETE IEVPHEETCD
160 170 180 190 200
TCHGSGAKPG TSPQSCPHCH GSGQVTSEQA TPFGRIVNRR TCPVCGGTGR
210 220 230 240 250
HIPEKCPTCG GTGHVKKRKK IHVKIPAGVD DGQQLRVAGK GEPGVNGGPP
260 270 280 290 300
GDLYIIFRVQ PHEFFKRDGD DIYCEVPLSF AQAALGDEIE VPTLHGDVKL
310 320 330 340 350
KIPAGTQTGT RFRLKGKGVP NVRGYGQGDQ HVIVRVVTPT KLTEKQKQLL
360 370 380
REFDRLGGET MHDGSHGRFF EKVKKAFKGE T
Length:381
Mass (Da):41,758
Last modified:May 1, 2007 - v1
Checksum:i679FEAD0E5BDB6F4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000557 Genomic DNA. Translation: ABO67784.1.
RefSeqiYP_001126529.1. NC_009328.1.

Genome annotation databases

EnsemblBacteriaiABO67784; ABO67784; GTNG_2439.
GeneIDi4967365.
KEGGigtn:GTNG_2439.
PATRICi21981417. VBIGeoThe136879_2570.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000557 Genomic DNA. Translation: ABO67784.1 .
RefSeqi YP_001126529.1. NC_009328.1.

3D structure databases

ProteinModelPortali A4IR30.
SMRi A4IR30. Positions 2-71.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 420246.GTNG_2439.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABO67784 ; ABO67784 ; GTNG_2439 .
GeneIDi 4967365.
KEGGi gtn:GTNG_2439.
PATRICi 21981417. VBIGeoThe136879_2570.

Phylogenomic databases

eggNOGi COG0484.
HOGENOMi HOG000226717.
KOi K03686.
OMAi GHTDPNQ.
OrthoDBi EOG6BPDKP.

Enzyme and pathway databases

BioCyci GTHE420246:GIXT-2516-MONOMER.

Family and domain databases

Gene3Di 1.10.287.110. 1 hit.
2.10.230.10. 1 hit.
HAMAPi MF_01152. DnaJ.
InterProi IPR012724. DnaJ.
IPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
IPR001305. HSP_DnaJ_Cys-rich_dom.
IPR001878. Znf_CCHC.
[Graphical view ]
Pfami PF01556. CTDII. 2 hits.
PF00226. DnaJ. 1 hit.
PF00684. DnaJ_CXXCXGXG. 1 hit.
[Graphical view ]
PRINTSi PR00625. JDOMAIN.
SMARTi SM00271. DnaJ. 1 hit.
SM00343. ZnF_C2HC. 1 hit.
[Graphical view ]
SUPFAMi SSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 3 hits.
SSF57938. SSF57938. 1 hit.
TIGRFAMsi TIGR02349. DnaJ_bact. 1 hit.
PROSITEi PS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51188. ZF_CR. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome and proteome of long-chain alkane degrading Geobacillus thermodenitrificans NG80-2 isolated from a deep-subsurface oil reservoir."
    Feng L., Wang W., Cheng J., Ren Y., Zhao G., Gao C., Tang Y., Liu X., Han W., Peng X., Liu R., Wang L.
    Proc. Natl. Acad. Sci. U.S.A. 104:5602-5607(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NG80-2.

Entry informationi

Entry nameiDNAJ_GEOTN
AccessioniPrimary (citable) accession number: A4IR30
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 1, 2007
Last modified: October 29, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3