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A4IR30

- DNAJ_GEOTN

UniProt

A4IR30 - DNAJ_GEOTN

Protein

Chaperone protein DnaJ

Gene

dnaJ

Organism
Geobacillus thermodenitrificans (strain NG80-2)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 59 (01 Oct 2014)
      Sequence version 1 (01 May 2007)
      Previous versions | rss
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    Functioni

    Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins.UniRule annotation

    Cofactori

    Binds 2 zinc ions per monomer.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi149 – 1491Zinc 1UniRule annotation
    Metal bindingi152 – 1521Zinc 1UniRule annotation
    Metal bindingi166 – 1661Zinc 2UniRule annotation
    Metal bindingi169 – 1691Zinc 2UniRule annotation
    Metal bindingi192 – 1921Zinc 2UniRule annotation
    Metal bindingi195 – 1951Zinc 2UniRule annotation
    Metal bindingi206 – 2061Zinc 1UniRule annotation
    Metal bindingi209 – 2091Zinc 1UniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri136 – 21883CR-typeUniRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: InterPro
    2. nucleic acid binding Source: InterPro
    3. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. DNA replication Source: UniProtKB-KW
    2. protein folding Source: InterPro
    3. response to heat Source: InterPro

    Keywords - Molecular functioni

    Chaperone

    Keywords - Biological processi

    DNA replication, Stress response

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciGTHE420246:GIXT-2516-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Chaperone protein DnaJUniRule annotation
    Gene namesi
    Name:dnaJUniRule annotation
    Ordered Locus Names:GTNG_2439
    OrganismiGeobacillus thermodenitrificans (strain NG80-2)
    Taxonomic identifieri420246 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus
    ProteomesiUP000001578: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 381381Chaperone protein DnaJPRO_1000085199Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi420246.GTNG_2439.

    Structurei

    3D structure databases

    ProteinModelPortaliA4IR30.
    SMRiA4IR30. Positions 2-71.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini5 – 6965JUniRule annotationAdd
    BLAST
    Repeati149 – 1568CXXCXGXG motif
    Repeati166 – 1738CXXCXGXG motif
    Repeati192 – 1998CXXCXGXG motif
    Repeati206 – 2138CXXCXGXG motif

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi76 – 11944Gly-richAdd
    BLAST

    Domaini

    The J domain is necessary and sufficient to stimulate DnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, DnaK-independent chaperone activity of DnaJ. Zinc center 2 is essential for interaction with DnaK and for DnaJ activity.UniRule annotation

    Sequence similaritiesi

    Belongs to the DnaJ family.UniRule annotation
    Contains 1 CR-type zinc finger.UniRule annotation
    Contains 1 J domain.UniRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri136 – 21883CR-typeUniRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG0484.
    HOGENOMiHOG000226717.
    KOiK03686.
    OMAiGHTDPNQ.
    OrthoDBiEOG6BPDKP.

    Family and domain databases

    Gene3Di1.10.287.110. 1 hit.
    2.10.230.10. 1 hit.
    HAMAPiMF_01152. DnaJ.
    InterProiIPR012724. DnaJ.
    IPR002939. DnaJ_C.
    IPR001623. DnaJ_domain.
    IPR018253. DnaJ_domain_CS.
    IPR008971. HSP40/DnaJ_pept-bd.
    IPR001305. HSP_DnaJ_Cys-rich_dom.
    IPR001878. Znf_CCHC.
    [Graphical view]
    PfamiPF01556. CTDII. 2 hits.
    PF00226. DnaJ. 1 hit.
    PF00684. DnaJ_CXXCXGXG. 1 hit.
    [Graphical view]
    PRINTSiPR00625. JDOMAIN.
    SMARTiSM00271. DnaJ. 1 hit.
    SM00343. ZnF_C2HC. 1 hit.
    [Graphical view]
    SUPFAMiSSF46565. SSF46565. 1 hit.
    SSF49493. SSF49493. 3 hits.
    SSF57938. SSF57938. 1 hit.
    TIGRFAMsiTIGR02349. DnaJ_bact. 1 hit.
    PROSITEiPS00636. DNAJ_1. 1 hit.
    PS50076. DNAJ_2. 1 hit.
    PS51188. ZF_CR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A4IR30-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAKRDYYEVL GVSKNATKDE IKKAYRKLSK QYHPDINKAP DAAEKFKEIK    50
    EAYEVLSDDE KRARYDRFGH ADPNEAFGGG FQGGGFDFGG FSGFGGFEDI 100
    FETFFGGGSR RRASGPRKGA DLEYMMTLTF EEAAFGKETE IEVPHEETCD 150
    TCHGSGAKPG TSPQSCPHCH GSGQVTSEQA TPFGRIVNRR TCPVCGGTGR 200
    HIPEKCPTCG GTGHVKKRKK IHVKIPAGVD DGQQLRVAGK GEPGVNGGPP 250
    GDLYIIFRVQ PHEFFKRDGD DIYCEVPLSF AQAALGDEIE VPTLHGDVKL 300
    KIPAGTQTGT RFRLKGKGVP NVRGYGQGDQ HVIVRVVTPT KLTEKQKQLL 350
    REFDRLGGET MHDGSHGRFF EKVKKAFKGE T 381
    Length:381
    Mass (Da):41,758
    Last modified:May 1, 2007 - v1
    Checksum:i679FEAD0E5BDB6F4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000557 Genomic DNA. Translation: ABO67784.1.
    RefSeqiYP_001126529.1. NC_009328.1.

    Genome annotation databases

    EnsemblBacteriaiABO67784; ABO67784; GTNG_2439.
    GeneIDi4967365.
    KEGGigtn:GTNG_2439.
    PATRICi21981417. VBIGeoThe136879_2570.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000557 Genomic DNA. Translation: ABO67784.1 .
    RefSeqi YP_001126529.1. NC_009328.1.

    3D structure databases

    ProteinModelPortali A4IR30.
    SMRi A4IR30. Positions 2-71.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 420246.GTNG_2439.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABO67784 ; ABO67784 ; GTNG_2439 .
    GeneIDi 4967365.
    KEGGi gtn:GTNG_2439.
    PATRICi 21981417. VBIGeoThe136879_2570.

    Phylogenomic databases

    eggNOGi COG0484.
    HOGENOMi HOG000226717.
    KOi K03686.
    OMAi GHTDPNQ.
    OrthoDBi EOG6BPDKP.

    Enzyme and pathway databases

    BioCyci GTHE420246:GIXT-2516-MONOMER.

    Family and domain databases

    Gene3Di 1.10.287.110. 1 hit.
    2.10.230.10. 1 hit.
    HAMAPi MF_01152. DnaJ.
    InterProi IPR012724. DnaJ.
    IPR002939. DnaJ_C.
    IPR001623. DnaJ_domain.
    IPR018253. DnaJ_domain_CS.
    IPR008971. HSP40/DnaJ_pept-bd.
    IPR001305. HSP_DnaJ_Cys-rich_dom.
    IPR001878. Znf_CCHC.
    [Graphical view ]
    Pfami PF01556. CTDII. 2 hits.
    PF00226. DnaJ. 1 hit.
    PF00684. DnaJ_CXXCXGXG. 1 hit.
    [Graphical view ]
    PRINTSi PR00625. JDOMAIN.
    SMARTi SM00271. DnaJ. 1 hit.
    SM00343. ZnF_C2HC. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46565. SSF46565. 1 hit.
    SSF49493. SSF49493. 3 hits.
    SSF57938. SSF57938. 1 hit.
    TIGRFAMsi TIGR02349. DnaJ_bact. 1 hit.
    PROSITEi PS00636. DNAJ_1. 1 hit.
    PS50076. DNAJ_2. 1 hit.
    PS51188. ZF_CR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome and proteome of long-chain alkane degrading Geobacillus thermodenitrificans NG80-2 isolated from a deep-subsurface oil reservoir."
      Feng L., Wang W., Cheng J., Ren Y., Zhao G., Gao C., Tang Y., Liu X., Han W., Peng X., Liu R., Wang L.
      Proc. Natl. Acad. Sci. U.S.A. 104:5602-5607(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: NG80-2.

    Entry informationi

    Entry nameiDNAJ_GEOTN
    AccessioniPrimary (citable) accession number: A4IR30
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 20, 2008
    Last sequence update: May 1, 2007
    Last modified: October 1, 2014
    This is version 59 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3