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A4IR30 (DNAJ_GEOTN) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Chaperone protein DnaJ
Gene names
Name:dnaJ
Ordered Locus Names:GTNG_2439
OrganismGeobacillus thermodenitrificans (strain NG80-2) [Complete proteome] [HAMAP]
Taxonomic identifier420246 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

Protein attributes

Sequence length381 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins By similarity. HAMAP-Rule MF_01152

Cofactor

Binds 2 zinc ions per monomer By similarity. HAMAP-Rule MF_01152

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01152

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01152.

Domain

The J domain is necessary and sufficient to stimulate DnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, DnaK-independent chaperone activity of DnaJ. Zinc center 2 is essential for interaction with DnaK and for DnaJ activity By similarity. HAMAP-Rule MF_01152

Sequence similarities

Belongs to the DnaJ family.

Contains 1 CR-type zinc finger.

Contains 1 J domain.

Ontologies

Keywords
   Biological processDNA replication
Stress response
   Cellular componentCytoplasm
   DomainRepeat
Zinc-finger
   LigandMetal-binding
Zinc
   Molecular functionChaperone
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processDNA replication

Inferred from electronic annotation. Source: UniProtKB-KW

protein folding

Inferred from electronic annotation. Source: InterPro

response to heat

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: InterPro

nucleic acid binding

Inferred from electronic annotation. Source: InterPro

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 381381Chaperone protein DnaJ HAMAP-Rule MF_01152
PRO_1000085199

Regions

Domain5 – 6965J
Repeat149 – 1568CXXCXGXG motif HAMAP-Rule MF_01152
Repeat166 – 1738CXXCXGXG motif HAMAP-Rule MF_01152
Repeat192 – 1998CXXCXGXG motif HAMAP-Rule MF_01152
Repeat206 – 2138CXXCXGXG motif HAMAP-Rule MF_01152
Zinc finger136 – 21883CR-type HAMAP-Rule MF_01152
Compositional bias76 – 11944Gly-rich HAMAP-Rule MF_01152

Sites

Metal binding1491Zinc 1 By similarity
Metal binding1521Zinc 1 By similarity
Metal binding1661Zinc 2 By similarity
Metal binding1691Zinc 2 By similarity
Metal binding1921Zinc 2 By similarity
Metal binding1951Zinc 2 By similarity
Metal binding2061Zinc 1 By similarity
Metal binding2091Zinc 1 By similarity

Sequences

Sequence LengthMass (Da)Tools
A4IR30 [UniParc].

Last modified May 1, 2007. Version 1.
Checksum: 679FEAD0E5BDB6F4

FASTA38141,758
        10         20         30         40         50         60 
MAKRDYYEVL GVSKNATKDE IKKAYRKLSK QYHPDINKAP DAAEKFKEIK EAYEVLSDDE 

        70         80         90        100        110        120 
KRARYDRFGH ADPNEAFGGG FQGGGFDFGG FSGFGGFEDI FETFFGGGSR RRASGPRKGA 

       130        140        150        160        170        180 
DLEYMMTLTF EEAAFGKETE IEVPHEETCD TCHGSGAKPG TSPQSCPHCH GSGQVTSEQA 

       190        200        210        220        230        240 
TPFGRIVNRR TCPVCGGTGR HIPEKCPTCG GTGHVKKRKK IHVKIPAGVD DGQQLRVAGK 

       250        260        270        280        290        300 
GEPGVNGGPP GDLYIIFRVQ PHEFFKRDGD DIYCEVPLSF AQAALGDEIE VPTLHGDVKL 

       310        320        330        340        350        360 
KIPAGTQTGT RFRLKGKGVP NVRGYGQGDQ HVIVRVVTPT KLTEKQKQLL REFDRLGGET 

       370        380 
MHDGSHGRFF EKVKKAFKGE T 

« Hide

References

[1]"Genome and proteome of long-chain alkane degrading Geobacillus thermodenitrificans NG80-2 isolated from a deep-subsurface oil reservoir."
Feng L., Wang W., Cheng J., Ren Y., Zhao G., Gao C., Tang Y., Liu X., Han W., Peng X., Liu R., Wang L.
Proc. Natl. Acad. Sci. U.S.A. 104:5602-5607(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NG80-2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000557 Genomic DNA. Translation: ABO67784.1.
RefSeqYP_001126529.1. NC_009328.1.

3D structure databases

ProteinModelPortalA4IR30.
SMRA4IR30. Positions 2-71.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING420246.GTNG_2439.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABO67784; ABO67784; GTNG_2439.
GeneID4967365.
KEGGgtn:GTNG_2439.
PATRIC21981417. VBIGeoThe136879_2570.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0484.
HOGENOMHOG000226717.
KOK03686.
OMAVDDGQQL.
OrthoDBEOG6BPDKP.
ProtClustDBPRK14280.

Enzyme and pathway databases

BioCycGTHE420246:GIXT-2516-MONOMER.

Family and domain databases

Gene3D1.10.287.110. 1 hit.
2.10.230.10. 1 hit.
HAMAPMF_01152. DnaJ.
InterProIPR012724. DnaJ.
IPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
IPR001305. HSP_DnaJ_Cys-rich_dom.
IPR001878. Znf_CCHC.
[Graphical view]
PfamPF01556. CTDII. 2 hits.
PF00226. DnaJ. 1 hit.
PF00684. DnaJ_CXXCXGXG. 1 hit.
[Graphical view]
PRINTSPR00625. JDOMAIN.
SMARTSM00271. DnaJ. 1 hit.
SM00343. ZnF_C2HC. 1 hit.
[Graphical view]
SUPFAMSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 3 hits.
SSF57938. SSF57938. 1 hit.
TIGRFAMsTIGR02349. DnaJ_bact. 1 hit.
PROSITEPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51188. ZF_CR. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDNAJ_GEOTN
AccessionPrimary (citable) accession number: A4IR30
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 1, 2007
Last modified: February 19, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families