A4IQK7 (NAMA_GEOTN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADPH dehydrogenase
EC=1.6.99.1

Gene names

Name:	namA
Ordered Locus Names:	GTNG_2262

Organism

Geobacillus thermodenitrificans (strain NG80-2) [Complete proteome] [HAMAP]

Taxonomic identifier

420246 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Geobacillus › Bacillus thermodenitrificans

Protein attributes

Sequence length	340 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reduction of the double bond of an array of alpha,beta-unsaturated aldehydes and ketones. It also reduces the nitro group of nitroester and nitroaromatic compounds. It could have a role in detoxification processes By similarity. HAMAP-Rule MF_01614
Catalytic activity	NADPH + acceptor = NADP+ + reduced acceptor. HAMAP-Rule MF_01614
Cofactor	FMN By similarity.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the NADH:flavin oxidoreductase/NADH oxidase family. NamA subfamily.

Ontologies

Keywords
Biological process	Detoxification
Ligand	FMN Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	response to toxin Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	FMN binding Inferred from electronic annotation. Source: HAMAP NADP binding Inferred from electronic annotation. Source: HAMAP NADPH dehydrogenase activity Inferred from electronic annotation. Source: HAMAP pentaerythritol trinitrate reductase activity Inferred from electronic annotation. Source: EC trichloro-p-hydroquinone reductive dehalogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 340	340	NADPH dehydrogenase HAMAP-Rule MF_01614		PRO_1000069457
Regions
	Nucleotide binding	23 – 27	5	FMN By similarity
Sites
	Binding site	28	1	Substrate By similarity
	Binding site	164	1	Substrate By similarity
	Binding site	167	1	Substrate By similarity
	Binding site	215	1	FMN By similarity
	Binding site	308	1	FMN By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4IQK7 [UniParc]. Last modified May 1, 2007. Version 1. Checksum: 99279A48DB537944	FASTA	340	37,782
	10 20 30 40 50 60 MNTVLFSPYT TRGLTLKNRI VMSPMCMYSC DTKDGTVRTW HKIHYPARAI GQVGLIIVEA 70 80 90 100 110 120 TGVTPQGRIS EHDLGIWDDD HIHGLHELVG LVKEHGAAIG IQLAHAGRKS EVPGEIIAPS 130 140 150 160 170 180 AIPFNESSPT PKEMTKADIE KTVQAFQDGA RRAKKAGFDV IEIHAAHGYL INEFLSPLSN 190 200 210 220 230 240 RRQDEYGGSP ENRYHFLGEV IDAVREVWDG PLFVRISASD YHPDGLTVKD YVPYVKRMKE 250 260 270 280 290 300 QGVDLVDVSS GAVVPARIDV YPGYQVPFAE FIRREAGIPT GAVGLITSGW QAEEVLHNGR 310 320 330 340 ADLVFLGREL LRNPYWPYAA AKELGVKIEA PVQYERGWRF

References

[1]

"Genome and proteome of long-chain alkane degrading Geobacillus thermodenitrificans NG80-2 isolated from a deep-subsurface oil reservoir." Feng L., Wang W., Cheng J., Ren Y., Zhao G., Gao C., Tang Y., Liu X., Han W., Peng X., Liu R., Wang L. Proc. Natl. Acad. Sci. U.S.A. 104:5602-5607(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NG80-2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000557 Genomic DNA. Translation: ABO67611.1.
RefSeq	YP_001126356.1. NC_009328.1.
3D structure databases
ProteinModelPortal	A4IQK7.
SMR	A4IQK7. Positions 5-338.
ModBase	Search...
Protein-protein interaction databases
STRING	420246.GTNG_2262.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABO67611; ABO67611; GTNG_2262.
GeneID	4967189.
KEGG	gtn:GTNG_2262.
PATRIC	21981049. VBIGeoThe136879_2386.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1902.
HOGENOM	HOG000116232.
OMA	EPYTLRQ.
ProtClustDB	PRK13523.
Enzyme and pathway databases
BioCyc	GTHE420246:GIXT-2340-MONOMER.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_01614. NamA.
InterPro	IPR013785. Aldolase_TIM. IPR023663. NADPH_DH. IPR001155. OxRdtase_FMN_N. [Graphical view]
Pfam	PF00724. Oxidored_FMN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAMA_GEOTN

Accession

Primary (citable) accession number: A4IQK7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	May 1, 2007
Last modified:	May 1, 2013
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program