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Protein

NADPH dehydrogenase

Gene

namA

Organism
Geobacillus thermodenitrificans (strain NG80-2)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reduction of the double bond of an array of alpha,beta-unsaturated aldehydes and ketones. It also reduces the nitro group of nitroester and nitroaromatic compounds. It could have a role in detoxification processes.UniRule annotation

Catalytic activityi

NADPH + acceptor = NADP+ + reduced acceptor.UniRule annotation

Cofactori

FMNUniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei28 – 281SubstrateUniRule annotation
Binding sitei164 – 1641SubstrateUniRule annotation
Binding sitei167 – 1671SubstrateUniRule annotation
Binding sitei215 – 2151FMNUniRule annotation
Binding sitei308 – 3081FMNUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi23 – 275FMNUniRule annotation

GO - Molecular functioni

  1. FMN binding Source: UniProtKB-HAMAP
  2. NADP binding Source: UniProtKB-HAMAP
  3. NADPH dehydrogenase activity Source: UniProtKB-HAMAP
  4. pentaerythritol trinitrate reductase activity Source: UniProtKB-EC
  5. trichloro-p-hydroquinone reductive dehalogenase activity Source: UniProtKB-EC

GO - Biological processi

  1. response to toxic substance Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Detoxification

Keywords - Ligandi

Flavoprotein, FMN, NADP

Enzyme and pathway databases

BioCyciGTHE420246:GIXT-2340-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH dehydrogenaseUniRule annotation (EC:1.6.99.1UniRule annotation)
Gene namesi
Name:namAUniRule annotation
Ordered Locus Names:GTNG_2262
OrganismiGeobacillus thermodenitrificans (strain NG80-2)
Taxonomic identifieri420246 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus
ProteomesiUP000001578 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 340340NADPH dehydrogenasePRO_1000069457Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi420246.GTNG_2262.

Structurei

3D structure databases

ProteinModelPortaliA4IQK7.
SMRiA4IQK7. Positions 5-338.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NADH:flavin oxidoreductase/NADH oxidase family. NamA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG1902.
HOGENOMiHOG000116232.
OMAiHILRTGQ.
OrthoDBiEOG60PHD0.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01614. NamA.
InterProiIPR013785. Aldolase_TIM.
IPR023663. NADPH_DH.
IPR001155. OxRdtase_FMN_N.
[Graphical view]
PfamiPF00724. Oxidored_FMN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4IQK7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTVLFSPYT TRGLTLKNRI VMSPMCMYSC DTKDGTVRTW HKIHYPARAI
60 70 80 90 100
GQVGLIIVEA TGVTPQGRIS EHDLGIWDDD HIHGLHELVG LVKEHGAAIG
110 120 130 140 150
IQLAHAGRKS EVPGEIIAPS AIPFNESSPT PKEMTKADIE KTVQAFQDGA
160 170 180 190 200
RRAKKAGFDV IEIHAAHGYL INEFLSPLSN RRQDEYGGSP ENRYHFLGEV
210 220 230 240 250
IDAVREVWDG PLFVRISASD YHPDGLTVKD YVPYVKRMKE QGVDLVDVSS
260 270 280 290 300
GAVVPARIDV YPGYQVPFAE FIRREAGIPT GAVGLITSGW QAEEVLHNGR
310 320 330 340
ADLVFLGREL LRNPYWPYAA AKELGVKIEA PVQYERGWRF
Length:340
Mass (Da):37,782
Last modified:May 1, 2007 - v1
Checksum:i99279A48DB537944
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000557 Genomic DNA. Translation: ABO67611.1.
RefSeqiYP_001126356.1. NC_009328.1.

Genome annotation databases

EnsemblBacteriaiABO67611; ABO67611; GTNG_2262.
KEGGigtn:GTNG_2262.
PATRICi21981049. VBIGeoThe136879_2386.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000557 Genomic DNA. Translation: ABO67611.1.
RefSeqiYP_001126356.1. NC_009328.1.

3D structure databases

ProteinModelPortaliA4IQK7.
SMRiA4IQK7. Positions 5-338.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi420246.GTNG_2262.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABO67611; ABO67611; GTNG_2262.
KEGGigtn:GTNG_2262.
PATRICi21981049. VBIGeoThe136879_2386.

Phylogenomic databases

eggNOGiCOG1902.
HOGENOMiHOG000116232.
OMAiHILRTGQ.
OrthoDBiEOG60PHD0.

Enzyme and pathway databases

BioCyciGTHE420246:GIXT-2340-MONOMER.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01614. NamA.
InterProiIPR013785. Aldolase_TIM.
IPR023663. NADPH_DH.
IPR001155. OxRdtase_FMN_N.
[Graphical view]
PfamiPF00724. Oxidored_FMN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome and proteome of long-chain alkane degrading Geobacillus thermodenitrificans NG80-2 isolated from a deep-subsurface oil reservoir."
    Feng L., Wang W., Cheng J., Ren Y., Zhao G., Gao C., Tang Y., Liu X., Han W., Peng X., Liu R., Wang L.
    Proc. Natl. Acad. Sci. U.S.A. 104:5602-5607(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NG80-2.

Entry informationi

Entry nameiNAMA_GEOTN
AccessioniPrimary (citable) accession number: A4IQK7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: May 1, 2007
Last modified: April 1, 2015
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.